@article{article,

title = {Development of Portable Fluorescence Microplate Reader Equipped with Indium Tin Oxide Glass Heater for Loop-mediated Isothermal Amplification},

author = {Ryo Ishii and Kazuhiro Morioka and Takuya Mizumoto and Natsumi Yamasaki and Akihide Hemmi and Atsushi Shoji and Hiroya Murakami and Norio Teshima and Tomonari Umemura and Katsumi Uchiyama and Hizuru Nakajima},

doi = {10.18494/SAM.2022.3618},

year  = {2022},

date = {2022-01-01},

journal = {Sensors and Materials},

volume = {34},

pages = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{MIYAUCHI2021102394,

title = {Development of an algal cell-attached solid surface culture system for simultaneous wastewater treatment and biomass production},

author = {Hiroki Miyauchi and Kohei Harada and Yoshino Suzuki and Katsuhiko Okada and Motohide Aoki and Tomonari Umemura and Shoko Fujiwara and Mikio Tsuzuki},

url = {https://www.sciencedirect.com/science/article/pii/S2211926421002137},

doi = {https://doi.org/10.1016/j.algal.2021.102394},

issn = {2211-9264},

year  = {2021},

date = {2021-07-06},

journal = {Algal Research},

volume = {58},

pages = {102394},

abstract = {Wastewater treatment using microalgae is receiving growing attention. Here, we have developed a portable tubular system containing an algal cell-coated solid surface for phosphorous recovery. P-depleted Chlorella cells attached to a solid surface removed phosphate from the medium about 70 times faster than P-replete cells. When the cell density was 20 g dry cell weight m−2 or less, P-depleted cells on the solid surface absorbed phosphate from the medium at almost the same rate as in liquid, the maximum capacity per solid surface area being about 6 mg P m−2 min−1. P in inorganic wastewater from chemical factories (ethanol factories; about 4 mg L−1) and in pond water (0.06 mg L−1) was mostly removed within 3 h with a simple device composed of a single solid-surfaced sheet (0.002 m−2) and a portable compact tubular device including 12 such sheets (totally 0.5 m−2), respectively. Simultaneously, cellular growth was confirmed with both wastewater and pond water. These findings suggested that the attached cell culture system, in which medium exchange to prepare P-depleted cells is much easier than in liquid cultures, is promising for dual use for biomass production and wastewater treatment.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{articleb,

title = {Development of a surface plasmon resonance sensor using an optical fiber prepared by electroless displacement gold plating and its application to immunoassay},

author = {Atsushi Shoji and Miyu Nakajima and Kazuhiro Morioka and Eiji Fujimori and Tomonari Umemura and Akio Yanagida and Akihide Hemmi and Katsumi Uchiyama and Hizuru Nakajima},

doi = {10.1016/j.talanta.2021.123162},

year  = {2021},

date = {2021-01-01},

journal = {Talanta},

volume = {240},

pages = {123162},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{articlec,

title = {Development of a fluorescence microplate reader using an organic photodiode array with a large light receiving area},

author = {Kazuhiro Morioka and Moeko Osashima and Nao Azuma and Kuizhi Qu and Akihide Hemmi and Atsushi Shoji and Hiroya Murakami and Norio Teshima and Tomonari Umemura and Katsumi Uchiyama and Hizuru Nakajima},

doi = {10.1016/j.talanta.2021.122994},

year  = {2021},

date = {2021-01-01},

journal = {Talanta},

volume = {238},

pages = {122994},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{articled,

title = {Preparation and Evaluation of Molding-type Solid-phase Extraction Media Binding with Commercially Available Adhesives},

author = {Yuta MIKI and Hiroya MURAKAMI and Keisuke IIDA and Tomonari UMEMURA and Yukihiro ESAKA and Yoshinori INOUE and Norio TESHIMA},

doi = {10.2116/analsci.21P265},

year  = {2021},

date = {2021-01-01},

journal = {Analytical Sciences},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Matsuo12.02.2020,

title = {Chemical Constituents of the Terrestrial Stems of Ephedra sinica and their PPAR-γ Ligand-Binding Activity},

author = {Yukiko Matsuo and Mayu Sasaki and Haruhiko Fukaya and Katsunori Miyake and Riko Takeuchi and Hidetoshi Kumata and Yoshihiro Mimaki},

url = {https://doi.org/10.1055/a-1094-9229},

doi = {10.1055/a-1094-9229},

issn = {2509-9264},

year  = {2020},

date = {2020-02-20},

journal = {Planta Medica International Open},

volume = {7},

number = {01},

pages = {e12-e16},

note = {e12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{KISHI2020113253,

title = {HPLC with electrochemical detection for determining homogentisic acid and its application to urine from rats fed tyrosine-enriched food},

author = {Takuho Kishi and Akira Kotani and Tomonari Umemura and Hideki Hakamata},

url = {http://www.sciencedirect.com/science/article/pii/S073170851932967X},

doi = {https://doi.org/10.1016/j.jpba.2020.113253},

issn = {0731-7085},

year  = {2020},

date = {2020-01-01},

journal = {Journal of Pharmaceutical and Biomedical Analysis},

volume = {186},

pages = {113253},

abstract = {A highly sensitive method for determining urine homogentisic acid (HGA) is required to provide adequate diagnosis and therapy for alkaptonuria in early stages. In this study, we developed a highly sensitive high-performance liquid chromatography with electrochemical detection (HPLC-ECD) for determining HGA in urine. In order to obtain a chromatogram of HGA by HPLC-ECD, an oxidation current was monitored at +0.5 V vs. Ag/AgCl. The peak heights of HGA showed linearity (r = 0.999) ranging from 4.2 ng/mL to 168 ng/mL, and the detection limit was 1.2 ng/mL (signal-to-noise ratio, S/N = 3). In recovery tests using human control urine spiked with an HGA standard, the recoveries of HGA were more than 93.2 %, and the relative standard deviations (n = 6) were less than 1.9 %. As an in vivo application using male Wistar rats, the level of urine HGA, which was metabolized from tyrosine in tyrosine-enriched food, was determined by this HPLC-ECD method. The determination of HGA in urine by this HPLC-ECD method requires only 0.1 mL of a rat urine specimen and simple sample preparation consisting of dilution and filtration.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{ENDO2020113448,

title = {Reduced bioavailability of polycyclic aromatic hydrocarbons (PAHs) in sediments impacted by carbon manufacturing plant effluent: Evaluation by ex situ passive sampling method},

author = {Satoshi Endo and Mitsuki Yoshimura and Hidetoshi Kumata and Masao Uchida and Yoshinori Yabuki and Haruhiko Nakata},

url = {http://www.sciencedirect.com/science/article/pii/S0269749119349796},

doi = {https://doi.org/10.1016/j.envpol.2019.113448},

issn = {0269-7491},

year  = {2020},

date = {2020-01-01},

journal = {Environmental Pollution},

volume = {256},

pages = {113448},

abstract = {Potential risks of polycyclic aromatic hydrocarbons (PAHs) in sediments of a Japanese bay contaminated by carbon manufacturing plant effluent were evaluated by calculating toxicity units (TUs). TUs calculated from the measured whole-sediment concentrations (Cwhole) were often higher than or close to 1, signaling a possible toxicity concern to benthic organisms. In contrast, TUs based on the freely dissolved pore water concentrations (Cfree) measured by an ex-situ passive sampling method with polyethylene strips were 0.0007–0.005, much lower than 1, indicating no effect. We also found that the fractions of black carbon in sediments of the contaminated bay were significantly higher than those of reference sites. Overall, we conclude that carbon manufacturing plant effluent substantially increases Cwhole of PAHs in sediments but also increases the fraction of carbonaceous particles that strongly retain PAHs. As a combined result, bioavailable concentrations (as expressed by pore water Cfree) of PAHs do not increase as much as Cwhole. The results of this study indicate that ecotoxicological risks of PAH contamination by carbon manufacturing plants should be evaluated by directly measuring pore water Cfree instead of Cwhole.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{曲奎智2020,

title = {自律送液が可能なマイクロチップと有機フォトダイオード検出器を用いる化学発光分析システムの開発},

author = {奎智 曲 and 和大 森岡 and 奈穗 東 and 萌子 長嶋 and 彰秀 辺見 and 敦 東海林 and 博哉 村上 and 紀雄 手嶋 and 知也 梅村 and 俊吾 加藤 and 奈保子 河西 and 一美 内山 and 秀 中嶋},

doi = {10.2116/bunsekikagaku.69.31},

year  = {2020},

date = {2020-01-01},

journal = {分析化学},

volume = {69},

number = {1.2},

pages = {31-39},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{MURAKAMI2020121052,

title = {Evaluation of the adsorption properties of nucleobase-modified sorbents for a solid-phase extraction of water-soluble compounds},

author = {Hiroya Murakami and Miho Omiya and Yuta Miki and Tomonari Umemura and Yukihiro Esaka and Yoshinori Inoue and Norio Teshima},

url = {http://www.sciencedirect.com/science/article/pii/S003991402030343X},

doi = {https://doi.org/10.1016/j.talanta.2020.121052},

issn = {0039-9140},

year  = {2020},

date = {2020-01-01},

journal = {Talanta},

volume = {217},

pages = {121052},

abstract = {We developed hydrophilic interaction chromatography (HILIC)-type sorbents modified with nucleobases for solid phase extraction (SPE). The synthesized hydrophilic base resins were modified by each nucleobase (adenine, guanine, and cytosine). The measurement of the amount of water content indicated that each nucleobase-modified sorbent had a water layer. To evaluate the adsorption properties in the HILIC mode, we chose two nucleobases (uracil and adenine) and four nucleosides (uridine, adenosine, cytidine, guanosine) as water-soluble analytes, which were loaded into an SPE cartridge packed with the nucleobase-modified sorbent. Firstly, 95% acetonitrile (ACN) solutions were used in the process of conditioning and sample loading of the above polar analytes. High recoveries of the analytes were observed in each nucleobase-modified sorbent, and the Diol-type sorbent (no modification with any of the nucleobases) did not adsorb each water-soluble analyte. On the basis of this result, a 98% ACN solution was used during the process of conditioning and sample loading to decrease the concentration of water in the sample, which potentially inhibited the formation of hydrogen bonding between each analyte and the modified nucleobase. Considerable improvements of recoveries were observed in Adenine- and Cytosine-modified sorbents. These results were possibly attributed to the effective expression of hydrogen bonding by decreasing water concentration in the sample solution. Although a non-aqueous (100% ACN) sample solution can be expected to obtain higher recoveries compared with the 98% ACN solution, a decrease in recoveries was observed in Adenine-modified sorbent. From these results, the highest adsorption property was observed in Adenine-modified sorbent using 98% ACN as a sample condition, and the combination of this sample condition and sorbent is effective for high adsorption under HILIC condition. Moreover, we also revealed that a balance between the thickness of water layer and the modification amount of nucleobase is important for retention in the HILIC-type sorbent.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{KUMATA2020100009,

title = {Elimination of interfering molybdenum oxyanion with an anion-exchange monolithic spin tip (AXTip) for precise determination of cadmium in human urine by ICP-MS},

author = {Hidetoshi Kumata and Chika Morimoto and Akihiro Horie and Akane Tanabe and Eiji Fujimori and Tomonari Umemura},

url = {http://www.sciencedirect.com/science/article/pii/S2666831920300096},

doi = {https://doi.org/10.1016/j.talo.2020.100009},

issn = {2666-8319},

year  = {2020},

date = {2020-01-01},

journal = {Talanta Open},

pages = {100009},

abstract = {A simple purification technique using an anion-exchange monolithic spin tip was described for the elimination of molybdenum oxyanion interfering with precise determination of cadmium in human urine samples by ICP-MS. The tip device named “anion-exchange tip-in monolith (AXTip)” was laboratory-prepared within the confines of a commercially available syringe filter tip by a two-step process: (1) in situ copolymerization of glycidyl methacrylate (GMA) and ethylene dimethacrylate (EDMA) and (2) its subsequent modification with trimethylamine (TMA), as a strong anion-exchange functional group, via ring-opening reaction of epoxide. The adsorption capacity of the AXTip was >3.2 μmol/tip for molybdenum oxyanion. The performance of the AXTip was evaluated through an adsorption/non-adsorption experiment for molybdenum, cadmium and other metal and metalloid species, using a model solution and human urine samples to optimize the sample solution's pH and dilution factor for the AXTip treatment. In the case of urine sample analysis, 8 times dilution and pH adjustment to 4.0 prior to AXTip treatment were found to allow >98 % elimination of interfering molybdenum oxyanions and quantitative recovery (103±7 %) of cadmium. At the optimized conditions, the AXTip treatment reduces Mo/Cd ratio from ∼400 in human urines to <10, which is sufficient for conducting ICP-MS measurement of cadmium in no-gas mode (without collision/reaction cell technique). The proposed purification technique was validated through the analysis of an accuracy control material of human urine (Seronorm™ Trace Elements Urine L-1). Good agreement of the observed values with the reference values indicates that the proposed technique is practically applicable.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{今崎龍之介2020,

title = {超薄層クロマトグラフィー用の水平式ミニチュアTLC展開槽の試作と評価},

author = {龍之介 今崎 and 啓太 近藤 and 夏海 谷 and 元秀 青木 and 英峰 熊田 and 達也 内田 and 豪 長縄 and 泰佑 嶋田 and 嘉彦 田口 and 浩明 佐藤 and 隆雄 安井 and 知也 梅村},

doi = {10.2116/bunsekikagaku.69.553},

year  = {2020},

date = {2020-01-01},

journal = {分析化学},

volume = {69},

number = {10.11},

pages = {553-558},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{articlef,

title = {The ER cholesterol sensor SCAP promotes CARTS biogenesis at ER–Golgi membrane contact sites},

author = {Yuichi Wakana and Kaito Hayashi and Takumi Nemoto and Chiaki Watanabe and Masato Taoka and Jessica Angulo-Capel and Maria Parajo and Hidetoshi Kumata and Tomonari Umemura and Hiroki Inoue and Kohei Arasaki and Felix Campelo and Mitsuo Tagaya},

doi = {10.1083/jcb.202002150},

year  = {2020},

date = {2020-01-01},

journal = {Journal of Cell Biology},

volume = {220},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Tani2019,

title = {Short-lived long noncoding RNAs as surrogate indicators for chemical stress in HepG2 cells and their degradation by nuclear RNases},

author = {Hidenori Tani and Ayaka Numajiri and Motohide Aoki and Tomonari Umemura and Tetsuya Nakazato},

url = {www.nature.com/scientificreports},

doi = {https://doi.org/10.1038/s41598-019-56869-y},

issn = {2045-2322},

year  = {2019},

date = {2019-12-30},

journal = {Scientific Reports},

volume = {9},

pages = {20299},

abstract = {Long noncoding RNAs (lncRNAs) are non-protein-coding transcripts >200 nucleotides in length that have been shown to play important roles in various biological processes. The mechanisms underlying the induction of lncRNA expression by chemical exposure remain to be determined. We identified a novel class of short-lived lncRNAs with half-lives (t1/2) ≤4 hours in human HeLa Tet-off cells, which have been suggested to express many lncRNAs with regulatory functions. As they may affect various human biological processes, short-lived lncRNAs may be useful indicators of the degree of stress on chemical exposure. In the present study, we identified four short-lived lncRNAs, designated as OIP5-AS1, FLJ46906, LINC01137, and GABPB1-AS1, which showed significantly upregulated expression following exposure to hydrogen peroxide (oxidative stress), mercury II chloride (heavy metal stress), and etoposide (DNA damage stress) in human HepG2 cells. These lncRNAs may be useful indicators of chemical stress responses. The levels of these lncRNAs in the cells were increased because of chemical stress-induced prolongation of their decay. These lncRNAs were degraded by nuclear RNases, which are components of the exosome and XRN2, and chemical exposure inhibited the RNase activities within the cells.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Umemura2019100101,

title = {Elucidation of Ion-Pairing Elution Behavior of Anions and Cations in Electrostatic Ion Chromatography Using Water as a Mobile Phase},

author = {Tomonari Umemura},

issn = {1342-8284},

year  = {2019},

date = {2019-10-01},

journal = {Chromatography},

volume = {40},

pages = {143-148},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{竹内理子2019,

title = {化合物レベル炭素安定同位体比分析のための高等植物中のステロール及び脂肪酸の分離精製法の確立},

author = {理子 竹内 and 嘉人 力石 and 奈々子 小川 and 郷史 風呂田 and 直彦 大河内 and 元秀 青木 and 達也 内田 and 知也 梅村 and 英峰 熊田},

doi = {10.2116/bunsekikagaku.68.297},

year  = {2019},

date = {2019-04-01},

journal = {分析化学},

volume = {68},

number = {5},

pages = {297-306},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{MUROTA2019101410,

title = {Hyper-resistance to arsenate in the cyanobacterium Synechocystis sp. PCC 6803 is influenced by the differential kinetics of its pst-ABC transporters and external phosphate concentration exposure},

author = {Chisato Murota and Shoko Fujiwara and Maki Tsujishita and Kanae Urabe and Shuta Takayanagi and Motohide Aoki and Tomonari Umemura and Julian J Eaton-Rye and Frances D Pitt and Mikio Tsuzuki},

url = {http://www.sciencedirect.com/science/article/pii/S2211926418307380},

doi = {https://doi.org/10.1016/j.algal.2019.101410},

issn = {2211-9264},

year  = {2019},

date = {2019-01-01},

journal = {Algal Research},

volume = {38},

pages = {101410},

abstract = {Phosphate transporters, which take up not only phosphate but also arsenate, have attracted attention for phosphorus (P) resource recovery from eutrophic environments and arsenic (As) removal from polluted areas by bioremediation. The cyanobacterium Synechocystis sp. PCC 6803 can grow in a higher ratio of arsenate (150 mM) to phosphate (0.2 mM) in comparison with plants and microalgae. Analysis of the As resistance of the Pst1 and Pst2 phosphate transporter deletion mutants (Δpst1 and Δpst2) showed that the Δpst1 strain is more sensitive to arsenate under P-replete conditions but more resistant under P-deplete conditions compared with Δpst2 cells and wild type. This pattern in sensitivity is attributed to the levels of initial As accumulation and pst gene expression. Moreover, the ratio of phosphate uptake to arsenate uptake rates at 10 μM was 7–10:1 in the wild type and the Δpst2 mutant, while that in Δpst1 and Δ pstS1 (phosphate-binding protein from the pst1 gene cluster), were about 1.5:1, suggesting a high selectivity of Pst1 for phosphate.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Nagashimae201900308,

title = {MITOL deletion in the brain impairs mitochondrial structure and ER tethering leading to oxidative stress},

author = {Shun Nagashima and Keisuke Takeda and Nobuhiko Ohno and Satoshi Ishido and Motohide Aoki and Yurika Saitoh and Takumi Takada and Takeshi Tokuyama and Ayumu Sugiura and Toshifumi Fukuda and Nobuko Matsushita and Ryoko Inatome and Shigeru Yanagi},

url = {https://www.life-science-alliance.org/content/2/4/e201900308},

doi = {10.26508/lsa.201900308},

year  = {2019},

date = {2019-01-01},

journal = {Life Science Alliance},

volume = {2},

number = {4},

publisher = {Life Science Alliance},

abstract = {Mitochondrial abnormalities are associated with developmental disorders, although a causal relationship remains largely unknown. Here, we report that increased oxidative stress in neurons by deletion of mitochondrial ubiquitin ligase MITOL causes a potential neuroinflammation including aberrant astrogliosis and microglial activation, indicating that mitochondrial abnormalities might confer a risk for inflammatory diseases in brain such as psychiatric disorders. A role of MITOL in both mitochondrial dynamics and ER-mitochondria tethering prompted us to characterize three-dimensional structures of mitochondria in vivo. In MITOL-deficient neurons, we observed a significant reduction in the ER-mitochondria contact sites, which might lead to perturbation of phospholipids transfer, consequently reduce cardiolipin biogenesis. We also found that branched large mitochondria disappeared by deletion of MITOL. These morphological abnormalities of mitochondria resulted in enhanced oxidative stress in brain, which led to astrogliosis and microglial activation partly causing abnormal behavior. In conclusion, the reduced ER-mitochondria tethering and excessive mitochondrial fission may trigger neuroinflammation through oxidative stress.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}