@article{article,

title = {Development of Portable Fluorescence Microplate Reader Equipped with Indium Tin Oxide Glass Heater for Loop-mediated Isothermal Amplification},

author = {Ryo Ishii and Kazuhiro Morioka and Takuya Mizumoto and Natsumi Yamasaki and Akihide Hemmi and Atsushi Shoji and Hiroya Murakami and Norio Teshima and Tomonari Umemura and Katsumi Uchiyama and Hizuru Nakajima},

doi = {10.18494/SAM.2022.3618},

year  = {2022},

date = {2022-01-01},

journal = {Sensors and Materials},

volume = {34},

pages = {1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{MIYAUCHI2021102394,

title = {Development of an algal cell-attached solid surface culture system for simultaneous wastewater treatment and biomass production},

author = {Hiroki Miyauchi and Kohei Harada and Yoshino Suzuki and Katsuhiko Okada and Motohide Aoki and Tomonari Umemura and Shoko Fujiwara and Mikio Tsuzuki},

url = {https://www.sciencedirect.com/science/article/pii/S2211926421002137},

doi = {https://doi.org/10.1016/j.algal.2021.102394},

issn = {2211-9264},

year  = {2021},

date = {2021-07-06},

journal = {Algal Research},

volume = {58},

pages = {102394},

abstract = {Wastewater treatment using microalgae is receiving growing attention. Here, we have developed a portable tubular system containing an algal cell-coated solid surface for phosphorous recovery. P-depleted Chlorella cells attached to a solid surface removed phosphate from the medium about 70 times faster than P-replete cells. When the cell density was 20 g dry cell weight m−2 or less, P-depleted cells on the solid surface absorbed phosphate from the medium at almost the same rate as in liquid, the maximum capacity per solid surface area being about 6 mg P m−2 min−1. P in inorganic wastewater from chemical factories (ethanol factories; about 4 mg L−1) and in pond water (0.06 mg L−1) was mostly removed within 3 h with a simple device composed of a single solid-surfaced sheet (0.002 m−2) and a portable compact tubular device including 12 such sheets (totally 0.5 m−2), respectively. Simultaneously, cellular growth was confirmed with both wastewater and pond water. These findings suggested that the attached cell culture system, in which medium exchange to prepare P-depleted cells is much easier than in liquid cultures, is promising for dual use for biomass production and wastewater treatment.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{articled,

title = {Preparation and Evaluation of Molding-type Solid-phase Extraction Media Binding with Commercially Available Adhesives},

author = {Yuta MIKI and Hiroya MURAKAMI and Keisuke IIDA and Tomonari UMEMURA and Yukihiro ESAKA and Yoshinori INOUE and Norio TESHIMA},

doi = {10.2116/analsci.21P265},

year  = {2021},

date = {2021-01-01},

journal = {Analytical Sciences},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{articlec,

title = {Development of a fluorescence microplate reader using an organic photodiode array with a large light receiving area},

author = {Kazuhiro Morioka and Moeko Osashima and Nao Azuma and Kuizhi Qu and Akihide Hemmi and Atsushi Shoji and Hiroya Murakami and Norio Teshima and Tomonari Umemura and Katsumi Uchiyama and Hizuru Nakajima},

doi = {10.1016/j.talanta.2021.122994},

year  = {2021},

date = {2021-01-01},

journal = {Talanta},

volume = {238},

pages = {122994},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{articleb,

title = {Development of a surface plasmon resonance sensor using an optical fiber prepared by electroless displacement gold plating and its application to immunoassay},

author = {Atsushi Shoji and Miyu Nakajima and Kazuhiro Morioka and Eiji Fujimori and Tomonari Umemura and Akio Yanagida and Akihide Hemmi and Katsumi Uchiyama and Hizuru Nakajima},

doi = {10.1016/j.talanta.2021.123162},

year  = {2021},

date = {2021-01-01},

journal = {Talanta},

volume = {240},

pages = {123162},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Matsuo12.02.2020,

title = {Chemical Constituents of the Terrestrial Stems of Ephedra sinica and their PPAR-γ Ligand-Binding Activity},

author = {Yukiko Matsuo and Mayu Sasaki and Haruhiko Fukaya and Katsunori Miyake and Riko Takeuchi and Hidetoshi Kumata and Yoshihiro Mimaki},

url = {https://doi.org/10.1055/a-1094-9229},

doi = {10.1055/a-1094-9229},

issn = {2509-9264},

year  = {2020},

date = {2020-02-20},

journal = {Planta Medica International Open},

volume = {7},

number = {01},

pages = {e12-e16},

note = {e12},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{articlef,

title = {The ER cholesterol sensor SCAP promotes CARTS biogenesis at ER–Golgi membrane contact sites},

author = {Yuichi Wakana and Kaito Hayashi and Takumi Nemoto and Chiaki Watanabe and Masato Taoka and Jessica Angulo-Capel and Maria Parajo and Hidetoshi Kumata and Tomonari Umemura and Hiroki Inoue and Kohei Arasaki and Felix Campelo and Mitsuo Tagaya},

doi = {10.1083/jcb.202002150},

year  = {2020},

date = {2020-01-01},

journal = {Journal of Cell Biology},

volume = {220},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{今崎龍之介2020,

title = {超薄層クロマトグラフィー用の水平式ミニチュアTLC展開槽の試作と評価},

author = {龍之介 今崎 and 啓太 近藤 and 夏海 谷 and 元秀 青木 and 英峰 熊田 and 達也 内田 and 豪 長縄 and 泰佑 嶋田 and 嘉彦 田口 and 浩明 佐藤 and 隆雄 安井 and 知也 梅村},

doi = {10.2116/bunsekikagaku.69.553},

year  = {2020},

date = {2020-01-01},

journal = {分析化学},

volume = {69},

number = {10.11},

pages = {553-558},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{KUMATA2020100009,

title = {Elimination of interfering molybdenum oxyanion with an anion-exchange monolithic spin tip (AXTip) for precise determination of cadmium in human urine by ICP-MS},

author = {Hidetoshi Kumata and Chika Morimoto and Akihiro Horie and Akane Tanabe and Eiji Fujimori and Tomonari Umemura},

url = {http://www.sciencedirect.com/science/article/pii/S2666831920300096},

doi = {https://doi.org/10.1016/j.talo.2020.100009},

issn = {2666-8319},

year  = {2020},

date = {2020-01-01},

journal = {Talanta Open},

pages = {100009},

abstract = {A simple purification technique using an anion-exchange monolithic spin tip was described for the elimination of molybdenum oxyanion interfering with precise determination of cadmium in human urine samples by ICP-MS. The tip device named “anion-exchange tip-in monolith (AXTip)” was laboratory-prepared within the confines of a commercially available syringe filter tip by a two-step process: (1) in situ copolymerization of glycidyl methacrylate (GMA) and ethylene dimethacrylate (EDMA) and (2) its subsequent modification with trimethylamine (TMA), as a strong anion-exchange functional group, via ring-opening reaction of epoxide. The adsorption capacity of the AXTip was >3.2 μmol/tip for molybdenum oxyanion. The performance of the AXTip was evaluated through an adsorption/non-adsorption experiment for molybdenum, cadmium and other metal and metalloid species, using a model solution and human urine samples to optimize the sample solution's pH and dilution factor for the AXTip treatment. In the case of urine sample analysis, 8 times dilution and pH adjustment to 4.0 prior to AXTip treatment were found to allow >98 % elimination of interfering molybdenum oxyanions and quantitative recovery (103±7 %) of cadmium. At the optimized conditions, the AXTip treatment reduces Mo/Cd ratio from ∼400 in human urines to <10, which is sufficient for conducting ICP-MS measurement of cadmium in no-gas mode (without collision/reaction cell technique). The proposed purification technique was validated through the analysis of an accuracy control material of human urine (Seronorm™ Trace Elements Urine L-1). Good agreement of the observed values with the reference values indicates that the proposed technique is practically applicable.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{MURAKAMI2020121052,

title = {Evaluation of the adsorption properties of nucleobase-modified sorbents for a solid-phase extraction of water-soluble compounds},

author = {Hiroya Murakami and Miho Omiya and Yuta Miki and Tomonari Umemura and Yukihiro Esaka and Yoshinori Inoue and Norio Teshima},

url = {http://www.sciencedirect.com/science/article/pii/S003991402030343X},

doi = {https://doi.org/10.1016/j.talanta.2020.121052},

issn = {0039-9140},

year  = {2020},

date = {2020-01-01},

journal = {Talanta},

volume = {217},

pages = {121052},

abstract = {We developed hydrophilic interaction chromatography (HILIC)-type sorbents modified with nucleobases for solid phase extraction (SPE). The synthesized hydrophilic base resins were modified by each nucleobase (adenine, guanine, and cytosine). The measurement of the amount of water content indicated that each nucleobase-modified sorbent had a water layer. To evaluate the adsorption properties in the HILIC mode, we chose two nucleobases (uracil and adenine) and four nucleosides (uridine, adenosine, cytidine, guanosine) as water-soluble analytes, which were loaded into an SPE cartridge packed with the nucleobase-modified sorbent. Firstly, 95% acetonitrile (ACN) solutions were used in the process of conditioning and sample loading of the above polar analytes. High recoveries of the analytes were observed in each nucleobase-modified sorbent, and the Diol-type sorbent (no modification with any of the nucleobases) did not adsorb each water-soluble analyte. On the basis of this result, a 98% ACN solution was used during the process of conditioning and sample loading to decrease the concentration of water in the sample, which potentially inhibited the formation of hydrogen bonding between each analyte and the modified nucleobase. Considerable improvements of recoveries were observed in Adenine- and Cytosine-modified sorbents. These results were possibly attributed to the effective expression of hydrogen bonding by decreasing water concentration in the sample solution. Although a non-aqueous (100% ACN) sample solution can be expected to obtain higher recoveries compared with the 98% ACN solution, a decrease in recoveries was observed in Adenine-modified sorbent. From these results, the highest adsorption property was observed in Adenine-modified sorbent using 98% ACN as a sample condition, and the combination of this sample condition and sorbent is effective for high adsorption under HILIC condition. Moreover, we also revealed that a balance between the thickness of water layer and the modification amount of nucleobase is important for retention in the HILIC-type sorbent.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{曲奎智2020,

title = {自律送液が可能なマイクロチップと有機フォトダイオード検出器を用いる化学発光分析システムの開発},

author = {奎智 曲 and 和大 森岡 and 奈穗 東 and 萌子 長嶋 and 彰秀 辺見 and 敦 東海林 and 博哉 村上 and 紀雄 手嶋 and 知也 梅村 and 俊吾 加藤 and 奈保子 河西 and 一美 内山 and 秀 中嶋},

doi = {10.2116/bunsekikagaku.69.31},

year  = {2020},

date = {2020-01-01},

journal = {分析化学},

volume = {69},

number = {1.2},

pages = {31-39},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{ENDO2020113448,

title = {Reduced bioavailability of polycyclic aromatic hydrocarbons (PAHs) in sediments impacted by carbon manufacturing plant effluent: Evaluation by ex situ passive sampling method},

author = {Satoshi Endo and Mitsuki Yoshimura and Hidetoshi Kumata and Masao Uchida and Yoshinori Yabuki and Haruhiko Nakata},

url = {http://www.sciencedirect.com/science/article/pii/S0269749119349796},

doi = {https://doi.org/10.1016/j.envpol.2019.113448},

issn = {0269-7491},

year  = {2020},

date = {2020-01-01},

journal = {Environmental Pollution},

volume = {256},

pages = {113448},

abstract = {Potential risks of polycyclic aromatic hydrocarbons (PAHs) in sediments of a Japanese bay contaminated by carbon manufacturing plant effluent were evaluated by calculating toxicity units (TUs). TUs calculated from the measured whole-sediment concentrations (Cwhole) were often higher than or close to 1, signaling a possible toxicity concern to benthic organisms. In contrast, TUs based on the freely dissolved pore water concentrations (Cfree) measured by an ex-situ passive sampling method with polyethylene strips were 0.0007–0.005, much lower than 1, indicating no effect. We also found that the fractions of black carbon in sediments of the contaminated bay were significantly higher than those of reference sites. Overall, we conclude that carbon manufacturing plant effluent substantially increases Cwhole of PAHs in sediments but also increases the fraction of carbonaceous particles that strongly retain PAHs. As a combined result, bioavailable concentrations (as expressed by pore water Cfree) of PAHs do not increase as much as Cwhole. The results of this study indicate that ecotoxicological risks of PAH contamination by carbon manufacturing plants should be evaluated by directly measuring pore water Cfree instead of Cwhole.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{KISHI2020113253,

title = {HPLC with electrochemical detection for determining homogentisic acid and its application to urine from rats fed tyrosine-enriched food},

author = {Takuho Kishi and Akira Kotani and Tomonari Umemura and Hideki Hakamata},

url = {http://www.sciencedirect.com/science/article/pii/S073170851932967X},

doi = {https://doi.org/10.1016/j.jpba.2020.113253},

issn = {0731-7085},

year  = {2020},

date = {2020-01-01},

journal = {Journal of Pharmaceutical and Biomedical Analysis},

volume = {186},

pages = {113253},

abstract = {A highly sensitive method for determining urine homogentisic acid (HGA) is required to provide adequate diagnosis and therapy for alkaptonuria in early stages. In this study, we developed a highly sensitive high-performance liquid chromatography with electrochemical detection (HPLC-ECD) for determining HGA in urine. In order to obtain a chromatogram of HGA by HPLC-ECD, an oxidation current was monitored at +0.5 V vs. Ag/AgCl. The peak heights of HGA showed linearity (r = 0.999) ranging from 4.2 ng/mL to 168 ng/mL, and the detection limit was 1.2 ng/mL (signal-to-noise ratio, S/N = 3). In recovery tests using human control urine spiked with an HGA standard, the recoveries of HGA were more than 93.2 %, and the relative standard deviations (n = 6) were less than 1.9 %. As an in vivo application using male Wistar rats, the level of urine HGA, which was metabolized from tyrosine in tyrosine-enriched food, was determined by this HPLC-ECD method. The determination of HGA in urine by this HPLC-ECD method requires only 0.1 mL of a rat urine specimen and simple sample preparation consisting of dilution and filtration.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Tani2019,

title = {Short-lived long noncoding RNAs as surrogate indicators for chemical stress in HepG2 cells and their degradation by nuclear RNases},

author = {Hidenori Tani and Ayaka Numajiri and Motohide Aoki and Tomonari Umemura and Tetsuya Nakazato},

url = {www.nature.com/scientificreports},

doi = {https://doi.org/10.1038/s41598-019-56869-y},

issn = {2045-2322},

year  = {2019},

date = {2019-12-30},

journal = {Scientific Reports},

volume = {9},

pages = {20299},

abstract = {Long noncoding RNAs (lncRNAs) are non-protein-coding transcripts >200 nucleotides in length that have been shown to play important roles in various biological processes. The mechanisms underlying the induction of lncRNA expression by chemical exposure remain to be determined. We identified a novel class of short-lived lncRNAs with half-lives (t1/2) ≤4 hours in human HeLa Tet-off cells, which have been suggested to express many lncRNAs with regulatory functions. As they may affect various human biological processes, short-lived lncRNAs may be useful indicators of the degree of stress on chemical exposure. In the present study, we identified four short-lived lncRNAs, designated as OIP5-AS1, FLJ46906, LINC01137, and GABPB1-AS1, which showed significantly upregulated expression following exposure to hydrogen peroxide (oxidative stress), mercury II chloride (heavy metal stress), and etoposide (DNA damage stress) in human HepG2 cells. These lncRNAs may be useful indicators of chemical stress responses. The levels of these lncRNAs in the cells were increased because of chemical stress-induced prolongation of their decay. These lncRNAs were degraded by nuclear RNases, which are components of the exosome and XRN2, and chemical exposure inhibited the RNase activities within the cells.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Umemura2019100101,

title = {Elucidation of Ion-Pairing Elution Behavior of Anions and Cations in Electrostatic Ion Chromatography Using Water as a Mobile Phase},

author = {Tomonari Umemura},

issn = {1342-8284},

year  = {2019},

date = {2019-10-01},

journal = {Chromatography},

volume = {40},

pages = {143-148},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{竹内理子2019,

title = {化合物レベル炭素安定同位体比分析のための高等植物中のステロール及び脂肪酸の分離精製法の確立},

author = {理子 竹内 and 嘉人 力石 and 奈々子 小川 and 郷史 風呂田 and 直彦 大河内 and 元秀 青木 and 達也 内田 and 知也 梅村 and 英峰 熊田},

doi = {10.2116/bunsekikagaku.68.297},

year  = {2019},

date = {2019-04-01},

journal = {分析化学},

volume = {68},

number = {5},

pages = {297-306},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Nagashimae201900308,

title = {MITOL deletion in the brain impairs mitochondrial structure and ER tethering leading to oxidative stress},

author = {Shun Nagashima and Keisuke Takeda and Nobuhiko Ohno and Satoshi Ishido and Motohide Aoki and Yurika Saitoh and Takumi Takada and Takeshi Tokuyama and Ayumu Sugiura and Toshifumi Fukuda and Nobuko Matsushita and Ryoko Inatome and Shigeru Yanagi},

url = {https://www.life-science-alliance.org/content/2/4/e201900308},

doi = {10.26508/lsa.201900308},

year  = {2019},

date = {2019-01-01},

journal = {Life Science Alliance},

volume = {2},

number = {4},

publisher = {Life Science Alliance},

abstract = {Mitochondrial abnormalities are associated with developmental disorders, although a causal relationship remains largely unknown. Here, we report that increased oxidative stress in neurons by deletion of mitochondrial ubiquitin ligase MITOL causes a potential neuroinflammation including aberrant astrogliosis and microglial activation, indicating that mitochondrial abnormalities might confer a risk for inflammatory diseases in brain such as psychiatric disorders. A role of MITOL in both mitochondrial dynamics and ER-mitochondria tethering prompted us to characterize three-dimensional structures of mitochondria in vivo. In MITOL-deficient neurons, we observed a significant reduction in the ER-mitochondria contact sites, which might lead to perturbation of phospholipids transfer, consequently reduce cardiolipin biogenesis. We also found that branched large mitochondria disappeared by deletion of MITOL. These morphological abnormalities of mitochondria resulted in enhanced oxidative stress in brain, which led to astrogliosis and microglial activation partly causing abnormal behavior. In conclusion, the reduced ER-mitochondria tethering and excessive mitochondrial fission may trigger neuroinflammation through oxidative stress.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{MUROTA2019101410,

title = {Hyper-resistance to arsenate in the cyanobacterium Synechocystis sp. PCC 6803 is influenced by the differential kinetics of its pst-ABC transporters and external phosphate concentration exposure},

author = {Chisato Murota and Shoko Fujiwara and Maki Tsujishita and Kanae Urabe and Shuta Takayanagi and Motohide Aoki and Tomonari Umemura and Julian J Eaton-Rye and Frances D Pitt and Mikio Tsuzuki},

url = {http://www.sciencedirect.com/science/article/pii/S2211926418307380},

doi = {https://doi.org/10.1016/j.algal.2019.101410},

issn = {2211-9264},

year  = {2019},

date = {2019-01-01},

journal = {Algal Research},

volume = {38},

pages = {101410},

abstract = {Phosphate transporters, which take up not only phosphate but also arsenate, have attracted attention for phosphorus (P) resource recovery from eutrophic environments and arsenic (As) removal from polluted areas by bioremediation. The cyanobacterium Synechocystis sp. PCC 6803 can grow in a higher ratio of arsenate (150 mM) to phosphate (0.2 mM) in comparison with plants and microalgae. Analysis of the As resistance of the Pst1 and Pst2 phosphate transporter deletion mutants (Δpst1 and Δpst2) showed that the Δpst1 strain is more sensitive to arsenate under P-replete conditions but more resistant under P-deplete conditions compared with Δpst2 cells and wild type. This pattern in sensitivity is attributed to the levels of initial As accumulation and pst gene expression. Moreover, the ratio of phosphate uptake to arsenate uptake rates at 10 μM was 7–10:1 in the wild type and the Δpst2 mutant, while that in Δpst1 and Δ pstS1 (phosphate-binding protein from the pst1 gene cluster), were about 1.5:1, suggesting a high selectivity of Pst1 for phosphate.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Fujimori2018,

title = {Investigation of adverse effect of coexisting aminopolycarboxylates on the determination of rare earth elements by ICP-MS after solid phase extraction using an iminodiacetate-based chelating-resin},

author = {Eiji Fujimori and Suzuka Nagata and Hidetoshi Kumata and Tomonari Umemura},

doi = {10.1016/j.chemosphere.2018.09.073},

year  = {2018},

date = {2018-09-14},

journal = {Chemosphere},

volume = {214},

pages = {288-294},

abstract = {When iminodiacetic acid chelating-resin solid phase extraction (SPE) was used for the preconcentration of rare earth elements (REEs) in river water samples around sewage treatment plant (STP), low recovery values for heavy REEs were observed. In order to find out the reason for the low recovery, in the present paper, organic ligands in the STP effluent, which may compete with iminodiacetic acids, were analyzed by GC-NPD. It was found that EDTA was contained in the STP effluent at several-100 nM level and interfered with the adsorption of REEs, especially heavy REEs (present at pM level) on the chelating-resin due to the formation of stable complexes. Therefore, acid treatment was applied to decompose EDTA molecules. As a result of acid treatment with HNO3 and H2O2 at 170 °C for 4 h, all REEs were almost quantitatively recovered from the STP effluent with chelating-resin SPE with good reproducibility. After the acid treatment and subsequent 40-times preconcentration with SPE, all REEs in river water samples were precisely determined by ICP-MS at several-10 to sub pg mL−1 levels.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Sakurada2018,

title = {Involvement of Acidic Polysaccharide Ph-PS-2 and Protein in Initiation of Coccolith Mineralization, as Demonstrated by In Vitro Calcification on the Base Plate},

author = {Shunto Sakurada and Shoko Fujiwara and Michio Suzuki and Toshihiro Kogure and Tatsuya Uchida and Tomonari Umemura and Mikio Tsuzuki},

url = {https://doi.org/10.1007/s10126-018-9818-4},

doi = {10.1007/s10126-018-9818-4},

issn = {1436-2236},

year  = {2018},

date = {2018-06-01},

journal = {Marine Biotechnology},

volume = {20},

number = {3},

pages = {304--312},

abstract = {Coccolithophorids, unicellular marine microalgae, have calcified scales with elaborate structures, called coccoliths, on the cell surface. Coccoliths generally comprise a base plate, CaCO3, and a crystal coat consisting of acidic polysaccharides. In this study, the in vitro calcification conditions on the base plate of Pleurochrysis haptonemofera were examined to determine the functions of the base plate and acidic polysaccharides (Ph-PS-1, -2, and -3). When EDTA-treated coccoliths (acidic polysaccharide-free base plates) or low pH-treated coccoliths (whole acidic polysaccharide-containing base plates) were used, mineralization was not detected on the base plate. In contrast, in the case of coccoliths which were decalcified by lowering of the pH and then treated with urea (Ph-PS-2-containing base plates), distinct aggregates, probably containing CaCO3, were observed only on the rim of the base plates. Energy dispersive X-ray spectroscopy (EDS) confirmed that the aggregates contained Ca and O, although X-ray diffraction analysis did not reveal any evidence of crystalline materials. Also, in vitro mineralization experiments performed on EDTA-treated coccoliths using isolated acidic polysaccharides demonstrated that the Ca-containing aggregates were markedly formed only in the presence of Ph-PS-2. Furthermore, in vitro mineralization experiments conducted on protein-extracted base plates suggested that the coccolith-associated protein(s) are involved in the Ca deposition. These findings suggest that Ph-PS-2 associated with the protein(s) on the base plate rim initiates Ca2+ binding at the beginning of coccolith formation, and some other factors are required for subsequent calcite formation.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Yagi2018,

title = {Selection of a platinum-binding sequence in a loop of a four-helix bundle protein.},

author = {Sota Yagi and Satoshi Akanuma and Asumi Kaji and Hiroya Niiro and Hayato Akiyama and Tatsuya Uchida and Akihiko. Yamagishi},

doi = {10.1016/j.jbiosc.2017.09.006},

issn = {1347-4421},

year  = {2018},

date = {2018-01-01},

journal = {Journal of Bioscience and Bioengineering},

volume = {125},

number = {2},

pages = {192--198},

publisher = {Society for Biotechnology, Japan},

abstract = {Protein-metal hybrids are functional materials with various industrial applications. For example, a redox enzyme immobilized on a platinum electrode is a key component of some biofuel cells and biosensors. To create these hybrid materials, protein mols. are bound to metal surfaces. Here, we report the selection of a novel platinum-binding sequence in a loop of a four-helix bundle protein, the Lac repressor four-helix protein (LARFH), an artificial protein in which four identical $alpha$-helixes are connected via three identical loops. We created a genetic library in which the Ser-Gly-Gln-Gly-Gly-Ser sequence within the first inter-helical loop of LARFH was semi-randomly mutated. The library was then subjected to selection for platinum-binding affinity by using the T7 phage display method. The majority of the selected variants contained the Tyr-Lys-Arg-Gly-Tyr-Lys (YKRGYK) sequence in their randomized segment. We characterized the platinum-binding properties of mutant LARFH by using quartz crystal microbalance anal. Mutant LARFH seemed to interact with platinum through its loop contg. the YKRGYK sequence, as judged by the estd. exclusive area occupied by a single mol. Furthermore, a 10-residue peptide contg. the YKRGYK sequence bound to platinum with reasonably high affinity and basic side chains in the peptide were crucial in mediating this interaction. In conclusion, we have identified an amino acid sequence, YKRGYK, in the loop of a helix-loop-helix motif that shows high platinum-binding affinity. This sequence could be grafted into loops of other polypeptides as an approach to immobilize proteins on platinum electrodes for use as biosensors among other applications. [on SciFinder(R)]},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Aoki2018,

title = {Fatty Acid Content and Composition of Triacylglycerols of Chlorella kessleri},

author = {Motohide Aoki and Norihiro Sato},

url = {http://www.bio-protocol.org/e2676},

doi = {10.21769/BioProtoc.2676},

issn = {2331-8325},

year  = {2018},

date = {2018-01-01},

journal = {Bio-Protocol},

volume = {7},

number = {1},

pages = {1--9},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Hirose2018,

title = {Electrochemically active bacteria sense electrode potentials for regulating catabolic pathways},

author = {Atsumi Hirose and Takuya Kasai and Motohide Aoki and Tomonari Umemura and Kazuya Watanabe and Atsushi Kouzuma},

url = {https://www.nature.com/articles/s41467-018-03416-4},

doi = {10.1038/s41467-018-03416-4},

issn = {2041-1723},

year  = {2018},

date = {2018-01-01},

journal = {Nature communications},

volume = {9},

number = {1},

pages = {1083},

abstract = {Electrochemically active bacteria (EAB) receive considerable attention for their utility in bioelectrochemical processes. Although electrode potentials are known to affect the metabolic activity of EAB, it is unclear whether EAB are able to sense and respond to electrode potentials. Here, we show that, in the presence of a high-potential electrode, a model EAB Shewanella oneidensis MR-1 can utilize NADH-dependent catabolic pathways and a background formate-dependent pathway to achieve high growth yield. We also show that an Arc regulatory system is involved in sensing electrode potentials and regulating the expression of catabolic genes, including those for NADH dehydrogenase. We suggest that these findings may facilitate the use of EAB in biotechnological processes and offer the molecular bases for their ecological strategies in natural habitats.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Tani2017,

title = {Short-lived long non-coding RNAs as surrogate indicators for chemical exposure and LINC00152 and MALAT1 modulate their neighboring genes},

author = {H Tani and S Okuda and K Nakamura and M Aoki and T Umemura},

doi = {https://doi.org/10.1371/journal.pone.0181628},

year  = {2017},

date = {2017-07-18},

journal = {PLoS One},

volume = {12},

number = {7},

pages = {e0181628},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Sakai2016,

title = {Electrochemical Determination of Synephrine by Hydrophilic Interaction Liquid Chromatography Using a Zwitterionic Monolith Column},

author = {Y Sakai and A Kotani and T Umemura and Y Mori and F Kusu and K Yamamoto and H Hakamata},

doi = {10.1002/elan.201600150},

year  = {2016},

date = {2016-06-06},

journal = {Electroanalysis},

volume = {28},

pages = {1947-1951},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Sato2016,

title = {Identification of genes for sulfolipid synthesis in primitive red alga Cyanidioschyzon merolae},

author = {Norihiro Sato and Satomi Kobayashi and Motohide Aoki and Tomonari Umemura and Isao Kobayashi and Mikio Tsuzuki},

url = {http://www.sciencedirect.com/science/article/pii/S0006291X16300067},

doi = {10.1016/j.bbrc.2016.01.006},

issn = {0006291X},

year  = {2016},

date = {2016-01-01},

journal = {Biochemical and Biophysical Research Communications},

volume = {470},

number = {1},

pages = {123--129},

publisher = {Elsevier Ltd},

abstract = {Sulfoquinovosyl diacylglycerol is one of the lipids that construct thylakoid membranes, and is distributed from cyanobacteria to plastids in plants including a red lineage. One of the most primitive red algae, Cyanidioschyzon melorae, similar to cyanobacteria and green plants, possesses homologs of the SQD1 and SQD2 genes that code for UDP-sulfoquinovose and sulfoquinovosyl diacylglycerol synthases, respectively, for the synthesis of sulfoquinovosyl diacylglycerol. We here revealed the structural properties of SQD1 and SQD2 homologs in C. melorae intrinsic to those of the authentic proteins, and verified their enzymatic functions through heterologous expression in cyanobacterial disruptants as to the corresponding genes. The results demonstrated that the system of sulfoqunovosyl diacylglycerol synthesis could have been conserved through evolution of cyanobacteria to plastids in a red lineage, which is compatible with the monophyletic origin of plastids.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Sakurai2016,

title = {Profiling of lipid and glycogen accumulations under different growth conditions in the sulfothermophilic red alga Galdieria sulphuraria},

author = {Toshihiro Sakurai and Motohide Aoki and Xiaohui Ju and Tatsuya Ueda and Yasunori Nakamura and Shoko Fujiwara and Tomonari Umemura and Mikio Tsuzuki and Ayumi Minoda},

url = {http://dx.doi.org/10.1016/j.biortech.2015.11.014},

doi = {10.1016/j.biortech.2015.11.014},

issn = {18732976},

year  = {2016},

date = {2016-01-01},

journal = {Bioresource Technology},

volume = {200},

pages = {861--866},

publisher = {Elsevier Ltd},

abstract = {The unicellular red alga Galdieria sulphuraria grows efficiently and produces a large amount of biomass in acidic conditions at high temperatures. It has great potential to produce biofuels and other beneficial compounds without becoming contaminated with other organisms. In G. sulphuraria, biomass measurements and glycogen and lipid analyses demonstrated that the amounts and compositions of glycogen and lipids differed when cells were grown under autotrophic, mixotrophic, and heterotrophic conditions. Maximum biomass production was obtained in the mixotrophic culture. High amounts of glycogen were obtained in the mixotrophic cultures, while the amounts of neutral lipids were similar between mixotrophic and heterotrophic cultures. The amounts of neutral lipids were highest in red algae, including thermophiles. Glycogen structure and fatty acids compositions largely depended on the growth conditions.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Chikamatsu2016,

title = {Optimization of the Extraction Solvent for Polycyclic Aromatic Hydrocarbons (PAHs) from Road Dusts by Using Pressurized Solvent Extraction and Evaluation of Its Impact on Source Diagnostic Isomer Pair Ratios of PAHs},

author = {Kazuhiro Chikamatsu and Motohide Aoki and Tatsuya Uchida and Tomonari Umemura and Hidetoshi Kumata},

year  = {2016},

date = {2016-01-01},

journal = {Bunseki Kagaku},

volume = {65},

number = {4},

pages = {193--202},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Honda2015,

title = {Study on chromium accumulation mechanism of Chinese break fern (Pteris vittata L.) by synchrotron radiation X-ray fluorescence analysis.},

author = {Mao Honda and Nobuyuki Kitajima and Tomoko Abe and Tomonari Umemura and Akiko. Hokura},

doi = {10.2116/bunsekikagaku.64.801},

issn = {0525-1931},

year  = {2015},

date = {2015-01-01},

journal = {Bunseki Kagaku},

volume = {64},

number = {11},

pages = {801--810},

publisher = {Nippon Bunseki Kagakkai},

abstract = {Pteris vittata L. is known as an arsenic hyper-accumulator. To understand the tolerance mechanism, this study investigated the speciation and distribution of chromium accumulated in fern using synchrotron-radiation X-ray fluorescence anal. The plants were sep. treated with K2Cr2O7 and CrCl3·H2O in a hydroponic system. The chem. speciations of chromium and sulfur in the fern were carried out by X-ray absorption near edge structure (XANES) anal.; the elemental distributions in their tissues were visualized by micro-XRF imaging. Chromium was highly accumulated in the roots. $mu$-XANES and XANES indicated that highly toxic Cr(VI) was reduced Cr(III) as less-toxic Cr(III). The fern-cultivated Cr(III) was accumulated as Cr(III). The chem. speciation of sulfur showed that the ratio of S(-II) compds., such as glutathione (GSH) and cysteine in the roots, increased when was treated with Cr(VI) and Cr(III). This result demonstrated that sulfur had a certain function in the detoxification process of P. vittata L. [on SciFinder(R)]},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Suzuki2015,

title = {Accumulation of Arsenic and Copper by Bryophytes Growing in an Aquatic Environment near Copper Mine Tailings.},

author = {Yusuke Suzuki and Chisato Takenaka and Rie Tomioka and Hiromi Tsubota and Yuka Takasaki and Tomonari. Umemura},

doi = {10.1007/s10230-015-0335-7},

issn = {1025-9112},

year  = {2015},

date = {2015-01-01},

journal = {Mine Water and the Environment},

pages = {Ahead of Print},

publisher = {Springer GmbH},

abstract = {Bryophytes with high As accumulation affinity were identified in the aquatic environment. We surveyed a stream near copper mine tailings and then conducted lab. expts. to confirm As accumulation in the bryophytes with high As affinity. We found that a moss, Scopelophila cataractae, accumulates As in addn. to Cu in aquatic environments and confirmed it in lab. expts. The highest value for As in S. cataractae from the field survey was 1300 mg/kg dry wt. at relatively low As concns. in the stream water (0.005 mg/L). In addn., Brachythecium plumosum and Rhynchostegium riparioides may also be useful bryophytes for accumulation of Cu and As, though the mechanisms of As accumulation might differ between these two bryophytes and S. cataractae. [on SciFinder(R)]},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Yanai2014,

title = {1,1-Bis(triflyl)alkadienes: Easy-To-Handle Building Blocks for Strongly Acidic Carbon Acids},

author = {Hikaru Yanai and Saki Egawa and Kenta Yamada and Junpei Ono and Motohide Aoki and Takashi Matsumoto and Takeo Taguchi},

url = {http://doi.wiley.com/10.1002/ajoc.201402010},

doi = {10.1002/ajoc.201402010},

issn = {21935807},

year  = {2014},

date = {2014-04-01},

journal = {Asian Journal of Organic Chemistry},

volume = {3},

number = {4},

pages = {556--563},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{2014cb,

title = {Discovery of the cyanobacterial biomarker respond to toxic metal based on the lipidomics},

author = {Motohide Aoki and Yoshisuke Nakamura and Runa Akamine and Hidetoshi Kumata and Tomonari Umemura},

isbn = {0916-717X},

year  = {2014},

date = {2014-01-01},

journal = {Biomedical Research on Trace Elements},

volume = {25},

number = {2},

pages = {83},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Inoue2013,

title = {Protein cohesion induced by metal ions observed with fluorescence correlation spectroscopy},

author = {Kana Inoue and Motohide Aoki and Kitao Fujiwara},

url = {http://www.tandfonline.com/doi/abs/10.1080/10934529.2013.781861},

doi = {10.1080/10934529.2013.781861},

issn = {1093-4529},

year  = {2013},

date = {2013-05-01},

journal = {Journal of Environmental Science and Health, Part A},

volume = {48},

number = {11},

pages = {1311--1317},

publisher = {Taylor & Francis},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Ohta2013,

title = {Lithium-ion conducting La2/3-xLi3xTiO3 solid electrolyte thin films with stepped and terraced surfaces [Retraction of document cited in CA156:547984].},

author = {Hiromichi Ohta and Teruyasu Mizoguchi and Noriyuki Aoki and Takashi Yamamoto and Akhmad Sabarudin and Tomonari. Umemura},

doi = {10.1063/1.4794148},

issn = {0003-6951},

year  = {2013},

date = {2013-01-01},

journal = {Applied Physics Letters},

volume = {102},

number = {8},

pages = {089902/1},

publisher = {American Institute of Physics},

abstract = {This article has been retracted due to an inapplicable measurement system. (c) 2013 American Institute of Physics. [on SciFinder(R)]},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Aoshima2013,

title = {A simple combination of higher-oxidation-state FeX3 and phosphine or amine ligand for living radical polymerization of styrene, methacrylate, and acrylate.},

author = {Hiroshi Aoshima and Kotaro Satoh and Tomonari Umemura and Masami. Kamigaito},

doi = {10.1039/c3py00352c},

issn = {1759-9962},

year  = {2013},

date = {2013-01-01},

journal = {Polymer Chemistry},

volume = {4},

number = {12},

pages = {3554--3562},

publisher = {Royal Society of Chemistry},

abstract = {Higher-oxidn.-state Fe halides [FeX3 (X = Cl, Br)] were employed in conjunction with ligands, mainly monodentate phosphines and amines, to effect the living radical polymn. of various vinyl monomers such as styrene, Me methacrylate (MMA), and Me acrylate (MA). Almost all combinations examd. could enable polymns. in the absence of exogenous reducing agents. However, appropriate combinations of FeX3 and ligands gave rise to polymers in a living manner, with controlled mol. wts. and narrow mol. wt. distributions (Mw/Mn = 1.1-1.2). Ligand combinations included FeCl3 with PnBu3, P(CMe3)3, or NnBu3 (for styrene); FeCl3 with P(CMe3)3 or NnBu3 (for MMA); and FeBr3 with PPh3 (for MA). Model reactions and spectroscopic anal. suggest that FeCl3 most likely disproportionates into the Fe(III)Cl4- anion and Fe(III)Cl2+ cation in the presence of Lewis base ligands (PR3 and NR3). The latter cationic species, coordinated with the ligand [Fe(III)Cl2(PR3)+ or Fe(II)Cl2(PR3)+], acts as the active catalyst. Assistance from the electron-rich ligand allows the catalyst to induce metal-catalyzed living radical polymn. The Fe(III)-based catalyst could also be easily and almost quant. removed from the polymer product simply by washing with aq. acid to minimize the amt. of Fe contamination (<5 ppm). [on SciFinder(R)]},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Yamamoto2013,

title = {Numerical simulation on flow in column chromatography.},

author = {Kazuhiro Yamamoto and Ryo Komiyama and Tomonari. Umemura},

doi = {10.1142/S0129183113400032},

issn = {0129-1831},

year  = {2013},

date = {2013-01-01},

journal = {International Journal of Modern Physics C: Computational Physics, Physical Computation},

volume = {24},

number = {12},

pages = {1340003/1--1340003/7},

publisher = {World Scientific Publishing Co. Pte. Ltd.},

abstract = {Monolithic columns have attracted much attention as a novel platform for high throughput anal., but there is little information about the fluid profile in the flow channels. In this paper, we presented our approach for the fluid simulation in column chromatog. by the lattice Boltzmann method (LBM). To simulate the monolithic column system, the calcn. domain was modeled by the 3D channel flow through sphere obstacles. Several types of porous structure were used, with uniform and nonuniform pores. Based on the simulations results, we discussed fluid flow and pressure variation for the optimization of the suitable structure for HPLC system. [on SciFinder(R)]},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Groombridge2013,

title = {High sensitive elemental analysis of single yeast cells (Saccharomyces cerevisiae) by time-resolved inductively-coupled plasma mass spectrometry using a high efficiency cell introduction system.},

author = {Alexander S Groombridge and Shin-ichi Miyashita and Shin-ichiro Fujii and Keisuke Nagasawa and Tetsuya Okahashi and Masaki Ohata and Tomonari Umemura and Akiko Takatsu and Kazumi Inagaki and Koichi. Chiba},

doi = {10.2116/analsci.29.597},

issn = {0910-6340},

year  = {2013},

date = {2013-01-01},

journal = {Analytical Sciences},

volume = {29},

number = {6},

pages = {597--603},

publisher = {Japan Society for Analytical Chemistry},

abstract = {Trace elemental anal. of single yeast cells with time-resolved inductively coupled plasma mass spectrometry (ICP-MS) was successfully carried out, where a high efficiency cell introduction system (HECIS) consisting of the high performance concentric nebulizer (HPCN) and a low-vol. (15 mL) on-axis spray chamber utilizing a sheath gas flow were used. Cell adsorption to the flow injector and sample tubing was reduced with the addn. of a simple 4.3 mmol L-1 of NaCl soln. to the cell suspension and cell flowing liq., allowing consecutive measurements without fear of significant contamination from previous measurements. Initially using a quadrupole mass analyzer ICP-MS (ICP-QMS) at its lowest integration time (10 ms), current spikes corresponding to sep. cell events were detected for several elements (Mg, P, Ca, Mn, Fe, Cu, and Zn) on the introduction of the cell suspension. On comparing the no. of peaks in the spectrum for phosphorous with the cell count using a haemocytometer, a reproducible cell transport efficiency of 75.0 ± 4.7% was achieved. Preliminary expts. into using time of flight ICP-MS (ICP-TOFMS) for single-cell anal. were carried out, allowing quasi-simultaneous multielement detection. The spectra of Mg, P, Ca, Mn, Fe, Cu, and Zn, with a time resoln. of 1 ms were simultaneously obtained in one measurement. A relatively strong correlation was obsd. for the spectra between P and Zn (correlation factor 0.69), P and Mg (0.63), and Mg and Zn (0.63). These results indicate that the time resolved quasi-simultaneous multielement measurement may be useful for the correlation anal. of multielements in cells. [on SciFinder(R)]},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Fujiwara2012b,

title = {Observation of Cell-Size Variation under Environmental Stress by Fluorescence Correlation Spectroscopy without Objective Image Magnification},

author = {Kitao Fujiwara and Keishiro Horiuchi and Sayuri Goryoda and Yasuhiro Hashidume and Naoko Horiguchi and Takeshi Miyakawa and Masako Takasu and Motohide Aoki},

doi = {10.17265/2162-5298/2012.03.011},

isbn = {1934-8932},

year  = {2012},

date = {2012-01-01},

journal = {Journal of environmental science and engineering A},

volume = {1},

number = {3},

pages = {364-370},

abstract = {Fluorescence correlation spectroscopy (FCS) without objective image magnification (without using con-focal microscope) was applied to observe the variation in cell size of Escherichia coli (E. coli) induced by the anti-cancer agent MitomycinC (MMC). In the system without image magnification followed in this study, the suspension of E. coli cells was stirred, and the difference in movement due to the different cell sizes induced by the compulsive solution flow was detected. The addition of 0.1-0.4 $mu$g/L of MMC elongated the E. coli cell length from about 3.6 to 7.8 $mu$m. The flow cell (i.d. = about 1 mm) also produced a size-dependent correlation curve. The present system is not based on single molecular FCS but is inexpensive and effective at observing the variation in cell size induced by environmental changes.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Takasaki2012,

title = {Development of salt-tolerance interface for an high performance liquid chromatography/inductively coupled plasma mass spectrometry system and its application to accurate quantification of DNA samples.},

author = {Yuka Takasaki and Shinnosuke Sakagawa and Kazumi Inagaki and Shin-ichiro Fujii and Akhmad Sabarudin and Tomonari Umemura and Hiroki. Haraguchi},

doi = {10.1016/j.aca.2011.11.039},

issn = {0003-2670},

year  = {2012},

date = {2012-01-01},

journal = {Analytica Chimica Acta},

volume = {713},

pages = {23--29},

publisher = {Elsevier B.V.},

abstract = {Accurate quantification of DNA is highly important in various fields. Detn. of phosphorus by ICP-MS is one of the most effective methods for accurate quantification of DNA due to the fixed stoichiometry of phosphate to this mol. In this paper, a smart and reliable method for accurate quantification of DNA fragments and oligodeoxythymidilic acids by hyphenated HPLC/ICP-MS equipped with a highly efficient interface device is presented. The interface was constructed of a home-made capillary-attached micronebulizer and temp.-controllable cyclonic spray chamber (IsoMist). As a sepn. column for DNA samples, home-made methacrylate-based weak anion-exchange monolith was employed. Some parameters, which include compn. of mobile phase, gradient program, inner and outer diams. of capillary, temp. of spray chamber etc., were optimized to find the best performance for sepn. and accurate quantification of DNA samples. The proposed system could achieve many advantages, such as total consumption for small amt. sample anal., salt-tolerance for hyphenated anal., high accuracy and precision for quant. anal. Using this proposed system, the samples of 20 bp DNA ladder (20, 40, 60, 80, 100, 120, 140, 160, 180, 200, 300, 400, 500 base pairs) and oligodeoxythymidilic acids (dT12-18) were rapidly sepd. and accurately quantified. [on SciFinder(R)]},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Ohta2012,

title = {Lithium-ion conducting La2/3-xLi3xTiO3 solid electrolyte thin films with stepped and terraced surfaces.},

author = {Hiromichi Ohta and Teruyasu Mizoguchi and Noriyuki Aoki and Takashi Yamamoto and Akhmad Sabarudin and Tomonari. Umemura},

doi = {10.1063/1.4709402},

issn = {0003-6951},

year  = {2012},

date = {2012-01-01},

journal = {Applied Physics Letters},

volume = {100},

number = {17},

pages = {173107/1--173107/4},

publisher = {American Institute of Physics},

abstract = {La2/3-xLi3xTiO3 (LLT},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Shu2012,

title = {Chemical anchoring of lauryl methacrylate-based reversed phase monolith to 1/16" o.d. polyetheretherketone tubing.},

author = {Shin Shu and Hiroharu Kobayashi and Masaki Okubo and Akhmad Sabarudin and Michio Butsugan and Tomonari. Umemura},

doi = {10.1016/j.chroma.2012.04.030},

issn = {0021-9673},

year  = {2012},

date = {2012-01-01},

journal = {Journal of Chromatography A},

volume = {1242},

pages = {59--66},

publisher = {Elsevier B.V.},

abstract = {The authors describe a method for the prepn. of easy-to-use reversed-phase monolithic microbore columns. Polyetheretherketone (PEEK) tubing with an outer diam. of 1/16" and an inner diam. of 1.0 mm was used as a column housing (empty column), and in it lauryl methacrylate (LMA) was copolymd. with ethylene dimethacrylate (EDMA). In order to chem. anchor the polymer monolith to the tube wall, the inner wall surface was pretreated by the following two-step procedure. (1) 50% sulfuric acid was filled into the PEEK tubing and left to stand for 6 h to generate sulfonate groups on the surface. (2) After washing with Milli-Q water, the sulfonated PEEK surface was brought into contact with 1 M glycidyl methacrylate in dichloromethane (or acetone) at 40° for 4 h to introduce methacryloyl groups via the reaction of sulfonate groups and epoxy groups. Mech. strength and column efficiency of the resulting monoliths were evaluated through the sepn. of a series of alkylbenzenes in acetonitrile-water (50:50, vol./vol.) eluent over the flow rate range of 50-750 $mu$L/min (corresponding to 1.7-25.5 mm/s). The poly(LMA-co-EDMA) monolith provided acceptable column efficiency of 2000 theor. plates/10 cm (HETP value of 50 $mu$m) for amylbenzene (sepn. factor k = 40) and low flow resistance of 0.5 MPa/10 cm at a normal flow rate of 50 $mu$L/min. The methacryloylated PEEK tubing tightly held the monolith, and the monolithic column exhibited good pressure resistance up to 15 MPa, allowing rapid sepn. at a 15-20 fold higher flow rate than normal. [on SciFinder(R)]},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Aoki2012,

title = {Involvement of sulfoquinovosyl diacylglycerol in DNA synthesis in Synechocystis sp. PCC 6803.},

author = {Motohide Aoki and Mikio Tsuzuki and Norihiro. Sato},

doi = {10.1186/1756-0500-5-98},

issn = {1756-0500},

year  = {2012},

date = {2012-01-01},

journal = {BMC Research Notes},

volume = {5},

pages = {98},

publisher = {BioMed Central Ltd.},

abstract = {Background: Sulfoquinovosyl diacylglycerol (SQDG) is present in the membranes of cyanobacteria and their postulated progeny, plastids, in plants. A cyanobacterium, Synechocystis sp. PCC 6803, requires SQDG for growth: its mutant (SD1) with the sqdB gene for SQDG synthesis disrupted can grow with external supplementation of SQDG. However, upon removal of SQDG from the medium, its growth is retarded, with a decrease in the cellular content of SQDG throughout cell division, and finally ceases. Concomitantly with the decrease in SQDG, the maximal activity of photosynthesis at high-light intensity is repressed by 40%. Findings: We investigated effects of SQDG-defect on physiol. aspects in Synechocystis with the use of SD1. SD1 cells defective in SQDG exhibited normal photosynthesis at low-light intensity as on culturing. Meanwhile, SD1 cells defective in SQDG were impaired in light-activated heterotrophic growth as well as in photoautotrophic growth. Flow cytometric anal. of the photoautotrophically growing cells gave similar cell size histograms for the wild type and SD1 supplemented with SQDG. However, the profile of SD1 defective in SQDG changed such that large part of the cell population was increased in size. Of particular interest was the microscopic observation that the mitotic index, i.e., population of dumbbell-like cells with a septum, increased from 14 to 29 % in the SD1 culture without SQDG. Flow cytometric anal. also showed that the enlarged cells of SD1 defective in SQDG contained high levels of Chl, however, the DNA content was low. Conclusions: Our expts. strongly support the idea that photosynthesis is not the limiting factor for the growth of SD1 defective in SQDG, and that SQDG is responsible for some physiol. fundamental process common to both photoautotrophic and light-activated heterotrophic growth. Our findings suggest that the SQDG-defect allows construction of the photosynthetic machinery at an elevated level for an increase in cell mass, but represses DNA synthesis. SQDG may be essential for normal replication of chromosomal DNA for completion of the cell cycle. [on SciFinder(R)]},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Fujiwara2012,

title = {Observation of Cell-Size Variation under Environmental Stress by Fluorescence Correlation Spectroscopy without Objective Image Magnification},

author = {Kitao Fujiwara and Keishiro Horiuchi and Sayuri Goryoda and Yasuhiro Hashidume and Naoko Horiguchi and Takeshi Miyakawa and Masako Takasu and Motohide Aoki and Sayuri Goryoda Yasuhiro Hashidume Naoko Horiguchi Takeshi Miyakawa Masako Takasu Kitao Fujiwara Keishiro Horiuchi and Motohide Aoki},

year  = {2012},

date = {2012-01-01},

journal = {Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering},

volume = {1},

number = {3},

pages = {364--370},

abstract = {Fluorescence correlation spectroscopy (FCS) without objective image magnification (without using con-focal microscope) was applied to observe the variation in cell size of Escherichia coli (E. coli) induced by the anti-cancer agent MitomycinC (MMC). In the system without image magnification followed in this study, the suspension of E. coli cells was stirred, and the difference in movement due to the different cell sizes induced by the compulsive solution flow was detected. The addition of 0.1-0.4 $mu$g/L of MMC elongated the E. coli cell length from about 3.6 to 7.8 $mu$m. The flow cell (i.d. = about 1 mm) also produced a size-dependent correlation curve. The present system is not based on single molecular FCS but is inexpensive and effective at observing the variation in cell size induced by environmental changes.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Sabarudin2012,

title = {Preparation of methacrylate-based anion-exchange monolithic microbore column for chromatographic separation of DNA fragments and oligonucleotides.},

author = {Akhmad Sabarudin and Junchao Huang and Shin Shu and Shinnosuke Sakagawa and Tomonari. Umemura},

doi = {10.1016/j.aca.2012.05.039},

issn = {0003-2670},

year  = {2012},

date = {2012-01-01},

journal = {Analytica Chimica Acta},

volume = {736},

pages = {108--114},

publisher = {Elsevier B.V.},

abstract = {In this paper, we report on the prepn. of a microbore-scale (1 mm i.d.) anion-exchange monolithic column suitable not only for anal. purposes but also for potentially preparative applications. In order to meet the conflicting requirements of high permeability and good mech. strength, the following two-step procedure was applied. First, an epoxy-contg. monolith was synthesized by in situ copolymn. of glycidyl methacrylate (GMA) and ethylene dimethacrylate (EDMA) within the confines of a silicosteel tubing of 1.02 mm i.d. and 1/16'' o.d. in the presence of a ternary porogenic mixt. of 1-propanol, 1,4-butanediol, and water. The monolithic matrix was subsequently converted into weak anion-exchanger via the ring-opening reaction of epoxy group with di-Et amine. The dynamic binding capacity was 21.4 mg mL-1 for bovine serum albumin (BSA) at 10% breakthrough. The morphol. and porous structure of this monolith were assessed by scanning electron microscope (SEM) and inverse size exclusion chromatog. (ISEC). To optimize the sepn. efficiency, the effects of various chromatog. parameters upon the sepn. of DNA fragments were investigated. The resulting monolithic anion exchanger demonstrated good potential for the sepn. of both single- and double-stranded DNA mols. using a gradient elution with NaCl in Tris-HCl buffer (20 mM). Oligodeoxythymidylic acids (dT12-dT18) were successfully resolved at pH 8, while the fragments of 20 bp DNA ladder, 100 bp DNA ladder, and pBR322-HaeIII digest were efficiently sepd. at pH 9. [on SciFinder(R)]},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Zhu2012,

title = {Relative enrichment of Mo in the radicle of peanut seed (Arachis hypogaea), observed by multi-elemental imagining with LA-ICP-MS.},

author = {Yanbei Zhu and Akiharu Hioki and Akihide Itoh and Tomonari Umemura and Hiroki Haraguchi and Koichi. Chiba},

doi = {10.2116/analsci.28.1121},

issn = {0910-6340},

year  = {2012},

date = {2012-01-01},

journal = {Analytical Sciences},

volume = {28},

number = {12},

pages = {1121--1124},

publisher = {Japan Society for Analytical Chemistry},

abstract = {The distribution of eleven elements in a peanut seed were obtained by elemental imaging with laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The distribution of Mo was significantly different to those of other elements, such as K, P, and Mg. It was also confirmed that this typical enrichment of Mo was not dependent on the region where the peanut seed was planted. The enrichment of Mo was obsd. in the radicle of peanut seed, and was further confirmed by the isotopic ratio of Mo. [on SciFinder(R)]},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Noor2012,

title = {Atomic mineral characteristics of Indonesian osteoporosis by high-resolution inductively coupled plasma mass spectrometry.},

author = {Zairin Noor and Sutiman Bambang Sumitro and Mohammad Hidayat and Agus Hadian Rahim and Akhmad Sabarudin and Tomonari. Umemura},

doi = {10.1100/2012/372972},

issn = {1537-744X},

year  = {2012},

date = {2012-01-01},

journal = {Scientific World Journal},

pages = {372972, 6 pp.},

publisher = {Scientific World Journal},

abstract = {Clin. research indicates that neg. calcium balance is assocd. with low bone mass, rapid bone loss and high fracture rates. However, some studies revealed that not only calcium is involved in bone strengthening as risk factor of fracture osteoporosis. Thus, in this report, the difference of metallic and nonmetallic elements in osteoporosis and normal bones was studied by high-resoln. inductively coupled plasma mass spectrometry (HR-ICP-MS). The influence of these elements on bone metabolic processes is also discussed. Inclusion criteria of bone samples consist of postmenopausal woman, trabecular bone fracture, normal and osteoporosis BMD value and no history of previous disease. The results showed that the concn. of B, Al, S, V, Co, Mo, Te, Ba, La, Ni, As and Ca/P ratio is higher in osteoporosis than normal. These at. minerals have neg. role to imbalance between bone resorption and bone formation activity. Conversely, concns. of Na, Mg, P, K, Ca, Cr, Pd, Ag, Mn, Fe, Cu, Zn, Rb, Sr, Pb and Se are lower in osteoporosis than in normal bones. Among these atoms, known to have important roles in bone structure, we found involvement of at. mineral and calcium which are considerable to contribute to osteoporotic phenomena. [on SciFinder(R)]},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Bratescu2011,

title = {Analysis of organic pollutant degradation in pulsed plasma by coherent anti-Stokes Raman spectroscopy.},

author = {Maria Antoneta Bratescu and Junko Hieda and Tomonari Umemura and Nagahiro Saito and Osamu. Takai},

doi = {10.1116/1.3569035},

issn = {0734-2101},

year  = {2011},

date = {2011-01-01},

journal = {Journal of Vacuum Science & Technology, A: Vacuum, Surfaces, and Films},

volume = {29},

number = {3},

pages = {031302/1--031302/7},

publisher = {American Institute of Physics},

abstract = {The degrdn. of p-benzoquinone (p-BQ) in H2O was studied by the coherent anti-Stokes Raman spectroscopy (CARS) method, in which the change of the anti-Stokes signal intensity corresponding to the vibrational transitions of the mol. is monitored during and after soln. plasma processing (SPP). In the beginning of SPP treatment, the CARS signal intensity of the ring vibrational mol. transitions at 1233 and 1660 cm-1 increases under the influence of the elec. field of the plasma, depending on the delay time between the plasma pulse and the laser firing pulse. At the same time, the plasma contributes to the degrdn. of p-BQ mols. by generating H and hydroxyl radicals, which decomp. p-BQ into different carboxylic acids. After SPP, the CARS signal intensity of the vibrational bands of p-BQ ceased and the degrdn. of p-BQ was confirmed by UV-visible absorption spectroscopy and liq. chromatog. anal. (c) 2011 American Institute of Physics. [on SciFinder(R)]},

keywords = {},

pubstate = {published},

tppubtype = {article}

}