{"id":51,"date":"2015-08-24T12:00:55","date_gmt":"2015-08-24T03:00:55","guid":{"rendered":"http:\/\/logos.ls.toyaku.ac.jp\/~bioanalchem\/?page_id=51"},"modified":"2022-03-17T13:05:34","modified_gmt":"2022-03-17T04:05:34","slug":"ach1","status":"publish","type":"page","link":"https:\/\/www.ls.toyaku.ac.jp\/~bioanalchem\/ach1\/","title":{"rendered":"\u539f\u8457\u8ad6\u6587"},"content":{"rendered":"
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129 entries<\/span> «<\/a> ‹<\/a> 1 of 3 ›<\/a> »<\/a> <\/div><\/div>\r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n \r\n
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2025<\/h3>\r\n <\/td>\r\n <\/tr>

Kumata, Hidetoshi; Ochiai, Haruka; Tanaka, Anna; Oda, Shiori; Ishikawa-Takata, Kazuko; Umemura, Tomonari<\/p>

Simple analytical method to determine urinary isotopic enrichment of phenylalanine by GC\/EI-MS coupled with pentafluorobenzyl derivatization<\/a> Journal Article<\/span> Forthcoming<\/span><\/p>

In: <\/span>Talanta, <\/span>vol. 287, <\/span>Forthcoming.<\/p>

Abstract<\/a><\/span> | Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

@article{nokey,
\r\ntitle = {Simple analytical method to determine urinary isotopic enrichment of phenylalanine by GC\/EI-MS coupled with pentafluorobenzyl derivatization},
\r\nauthor = {Hidetoshi Kumata and Haruka Ochiai and Anna Tanaka and Shiori Oda and Kazuko Ishikawa-Takata and Tomonari Umemura},
\r\ndoi = {10.1016\/j.talanta.2025.127697},
\r\nyear  = {2025},
\r\ndate = {2025-05-15},
\r\nurldate = {2025-05-15},
\r\njournal = {Talanta},
\r\nvolume = {287},
\r\nabstract = {The indicator amino acid oxidation (IAAO) technique estimates the physiological requirements for amino acids and proteins in living organisms, including humans. It involves monitoring urinary amino acids and exhaled CO2 after ingesting 1-13C-labeled (carboxy-labeled) amino acids. The most common IAAO indicator amino acid is 1-13C-labeled phenylalanine ([1-13C]Phe). Its urinary concentration in test subjects ranges from below the detection limit to several \u03bcM. A simple analytical method for distinguishing trace amounts of [1-13C]Phe in urine from high levels of naturally occurring Phe is crucial for making IAAO tests easier. This study presents a simple and reliable approach for the simultaneous quantification of [1-13C]Phe and Phe in human urine using conventional GC-EI-MS. In this method, urinary phenylalanine is reacted with pentafluorobenzyl bromide in a single-phase solvent system of acetone-borate buffer without dehydration or desalting to form disubstituted pentafluorobenzyl (PFB) derivatives, which are then analyzed by GC-EI-MS (SIM). The Phe and [1-13C]Phe PFB derivative peaks eluted at the same retention time on the gas chromatogram but could be differentiated on the basis of fragment ions (m\/z 434, 435) derived from the loss of the phenyl group ([M \u2212 91]+). Correcting the interference of the m+1 isotope peak of Phe in the [M \u2212 91] fragment (m\/z 435) of [1-13C]Phe using the m\/z 434 peak intensity and natural isotope ratio, both Phe and [1-13C]Phe could be quantified in the concentration range found in urine. The method was successfully applied to examine the temporal enrichment of [1-13C]Phe in urine samples obtained from IAAO subjects following the ingestion of a test meal containing [1-13C]Phe.},
\r\nkeywords = {},
\r\npubstate = {forthcoming},
\r\ntppubtype = {article}
\r\n}
\r\n<\/pre><\/div>
Close<\/a><\/p><\/div>
The indicator amino acid oxidation (IAAO) technique estimates the physiological requirements for amino acids and proteins in living organisms, including humans. It involves monitoring urinary amino acids and exhaled CO2 after ingesting 1-13C-labeled (carboxy-labeled) amino acids. The most common IAAO indicator amino acid is 1-13C-labeled phenylalanine ([1-13C]Phe). Its urinary concentration in test subjects ranges from below the detection limit to several \u03bcM. A simple analytical method for distinguishing trace amounts of [1-13C]Phe in urine from high levels of naturally occurring Phe is crucial for making IAAO tests easier. This study presents a simple and reliable approach for the simultaneous quantification of [1-13C]Phe and Phe in human urine using conventional GC-EI-MS. In this method, urinary phenylalanine is reacted with pentafluorobenzyl bromide in a single-phase solvent system of acetone-borate buffer without dehydration or desalting to form disubstituted pentafluorobenzyl (PFB) derivatives, which are then analyzed by GC-EI-MS (SIM). The Phe and [1-13C]Phe PFB derivative peaks eluted at the same retention time on the gas chromatogram but could be differentiated on the basis of fragment ions (m\/z 434, 435) derived from the loss of the phenyl group ([M \u2212 91]+). Correcting the interference of the m+1 isotope peak of Phe in the [M \u2212 91] fragment (m\/z 435) of [1-13C]Phe using the m\/z 434 peak intensity and natural isotope ratio, both Phe and [1-13C]Phe could be quantified in the concentration range found in urine. The method was successfully applied to examine the temporal enrichment of [1-13C]Phe in urine samples obtained from IAAO subjects following the ingestion of a test meal containing [1-13C]Phe.<\/div>
Close<\/a><\/p><\/div>

\u9752\u6728, \u5143\u79c0<\/p>

\u30ea\u30d4\u30c9\u30df\u30af\u30b9\u3067\u7d10\u89e3\u304f\u7d30\u80de\u5185\u5171\u751f\u306e\u30c0\u30a4\u30ca\u30df\u30af\u30b9 \u2015\u751f\u4f53\u819c\u8102\u8cea\u306e\u52d5\u614b\u89e3\u6790\u304b\u3089\u5171\u751f\u6210\u7acb\u306e\u9375\u3092\u63a2\u308b Journal Article<\/span> <\/p>

In: <\/span>\u7d30\u80de, <\/span>vol. 57, <\/span>iss. 5, <\/span>pp. 80-81, <\/span>2025<\/span>, ISSN: 1346-7557<\/span>.<\/p>

BibTeX<\/a><\/span><\/p>

Ito, Ryo; Endo, Mizuki; Aoki, Motohide; Fujiwara, Shoko; Sato, Norihiro<\/p>

Evolutionary conservation of acylplastoquinone species from cyanobacteria to eukaryotic photosynthetic organisms of green and red lineages<\/a> Journal Article<\/span> <\/p>

In: <\/span>Frontiers in Plant Science, <\/span>vol. Volume 16 - 2025, <\/span>2025<\/span>, ISSN: 1664-462X<\/span>.<\/p>

Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

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2024<\/h3>\r\n <\/td>\r\n <\/tr>

KUMATA, Hidetoshi; UCHIDA, Masao; SAHA, Mahua; SAITOH, Shoichi; MANTOKU, Kanako; KOBAYASHI, Toshiyuki; OKUDA, Tomoaki; NAKAJIMA, Fumiyuki; HATAKEYAMA, Shiro; SHIBATA, Yasuyuki; TAKADA, Hideshige<\/p>

Source apportionment of atmospheric and sedimentary PAHs from Kolkata, India using compound-class-specific radiocarbon analysis (CCSRA)<\/a> Journal Article<\/span> <\/p>

In: <\/span>Radiocarbon, <\/span>vol. 66, <\/span>iss. 5, <\/span>pp. 892-903, <\/span>2024<\/span>.<\/p>

Abstract<\/a><\/span> | Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

@article{nokey,
\r\ntitle = {Source apportionment of atmospheric and sedimentary PAHs from Kolkata, India using compound-class-specific radiocarbon analysis (CCSRA)},
\r\nauthor = {Hidetoshi KUMATA and Masao UCHIDA and Mahua SAHA and Shoichi SAITOH and Kanako
\r\n MANTOKU and Toshiyuki KOBAYASHI and Tomoaki OKUDA and Fumiyuki NAKAJIMA and Shiro
\r\n HATAKEYAMA and Yasuyuki SHIBATA and Hideshige TAKADA},
\r\nurl = {https:\/\/doi.org\/10.1017\/RDC.2024.66},
\r\ndoi = {10.1017\/RDC.2024.66},
\r\nyear  = {2024},
\r\ndate = {2024-09-18},
\r\nurldate = {2024-09-24},
\r\njournal = {Radiocarbon},
\r\nvolume = {66},
\r\nissue = {5},
\r\npages = {892-903},
\r\nabstract = {Polycyclic aromatic hydrocarbons (PAHs) are major air pollutants that are ubiquitously produced by the combustion of organic materials, and it is extremely important to identify their pollution sources. In this study, molecular fingerprinting and compound class-specific radiocarbon dating (CCSRA) were performed on PAHs from canal sediments and air samples collected in Kolkata, India\u2019s third largest city (population approximately 16 million), where PAHs pollution has been a serious problem. Average PAH (\u03a312-parent PAHs) concentrations in air samples were 65.1 ng m\u20133 in summer and 70.9 ng m\u20133 in winter and in canal sediments were 32.7 \u00b5g g\u20131, which are classified as \u201cvery high-level\u201d pollution. Molecular fingerprinting using methyl-PAH\/PAH (MPAHs\/PAHs) ratios and isomer pair ratios with molecular weights of 178, 202, 228, and 276 suggested that wood and coal combustion were the dominant sources of PAHs in the sediment, and that atmospheric PAHs were influenced by oil combustion in addition to them. The fraction of contemporary carbon (\u0192C) of sedimentary PAHs (0.056\u20130.100), together with the extremely low MPAHs\/PAHs ratio results, lead to the conclusion that the major source of the high concentration of PAHs in the canals is from coal combustion. On the other hand, the \u0192C of atmospheric PAHs (0.272\u20130.369) was close to the share of biomass fuels in India\u2019s domestic fuel consumption in 2011 (about 35%). Furthermore, the observed \u0192C-discrepancy between atmospheric and sedimentary PAHs in the same urban environment was interpreted to give an insight into the loading pathway of PAHs to canal sediments in Kolkata.},
\r\nkeywords = {},
\r\npubstate = {published},
\r\ntppubtype = {article}
\r\n}
\r\n<\/pre><\/div>
Close<\/a><\/p><\/div>
Polycyclic aromatic hydrocarbons (PAHs) are major air pollutants that are ubiquitously produced by the combustion of organic materials, and it is extremely important to identify their pollution sources. In this study, molecular fingerprinting and compound class-specific radiocarbon dating (CCSRA) were performed on PAHs from canal sediments and air samples collected in Kolkata, India\u2019s third largest city (population approximately 16 million), where PAHs pollution has been a serious problem. Average PAH (\u03a312-parent PAHs) concentrations in air samples were 65.1 ng m\u20133 in summer and 70.9 ng m\u20133 in winter and in canal sediments were 32.7 \u00b5g g\u20131, which are classified as \u201cvery high-level\u201d pollution. Molecular fingerprinting using methyl-PAH\/PAH (MPAHs\/PAHs) ratios and isomer pair ratios with molecular weights of 178, 202, 228, and 276 suggested that wood and coal combustion were the dominant sources of PAHs in the sediment, and that atmospheric PAHs were influenced by oil combustion in addition to them. The fraction of contemporary carbon (\u0192C) of sedimentary PAHs (0.056\u20130.100), together with the extremely low MPAHs\/PAHs ratio results, lead to the conclusion that the major source of the high concentration of PAHs in the canals is from coal combustion. On the other hand, the \u0192C of atmospheric PAHs (0.272\u20130.369) was close to the share of biomass fuels in India\u2019s domestic fuel consumption in 2011 (about 35%). Furthermore, the observed \u0192C-discrepancy between atmospheric and sedimentary PAHs in the same urban environment was interpreted to give an insight into the loading pathway of PAHs to canal sediments in Kolkata.<\/div>
Close<\/a><\/p><\/div>

Ogawa, Tadayuki; Homma-Takeda, Shino; Umemura, Tomonari; Hirata, Takafumi; Saito, Yuko<\/p>

Molecular environment in Alzheimer\u2019s disease brain revealed by the hybrid imaging analyses<\/a> Journal Article<\/span> <\/p>

In: <\/span>Alzheimer's & Dementia, <\/span>vol. 20, <\/span>no. S1, <\/span>pp. e093362, <\/span>2024<\/span>.<\/p>

Abstract<\/a><\/span> | Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

@article{https:\/\/doi.org\/10.1002\/alz.093362,
\r\ntitle = {Molecular environment in Alzheimer\u2019s disease brain revealed by the hybrid imaging analyses},
\r\nauthor = {Tadayuki Ogawa and Shino Homma-Takeda and Tomonari Umemura and Takafumi Hirata and Yuko Saito},
\r\nurl = {https:\/\/alz-journals.onlinelibrary.wiley.com\/doi\/abs\/10.1002\/alz.093362},
\r\ndoi = {https:\/\/doi.org\/10.1002\/alz.093362},
\r\nyear  = {2024},
\r\ndate = {2024-01-01},
\r\njournal = {Alzheimer's & Dementia},
\r\nvolume = {20},
\r\nnumber = {S1},
\r\npages = {e093362},
\r\nabstract = {Abstract Background Although amyloid deposition in brain is one of the hallmark pathological features of Alzheimer\u2019s disease (AD), the upstream events and its molecular environment in AD brain remain largely unknown. Recent advances in analytical methods such as mass spectrometry can provide the cutting-edge tools to unveil the AD pathogenesis at molecular and atomic level. Method In order to gain the comprehensive information about AD pathology at molecular level, postmortem brain sections of AD patients were analyzed by the hybrid molecular imaging methods composed of the conventional histological analyses, matrix assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) for small molecules, laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) imaging for metals, and particle induced X-ray emission (PIXE) imaging for elements. Principal components analysis (PCA) was also applied to discover the differences between the spectra datasets of AD and healthy control brains. Specific distribution of molecules and elements were visualized on the brain slice. (Ethics Approval: Dokkyo Medical University No.2021-004, Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology No.R20-003). Result MALDI-MSI showed abnormal distribution of lipids, such as phospholipids, sphingolipids, and their derivatives in AD brain. LA-ICP-MS visualized the distribution of elements, including some metals in the wide-field brain section. PIXE imaging displayed the specific concentration of atoms nearby the pathological tangles detected by anti-ubiquitin antibody. PCA proposed the effective classification to distinguish AD from control based on mass spectra. Conclusion Our data suggest the molecular and atomic environment in brain that is closely linked to AD pathology. Further analyses should be conducted to elucidate the fundamental mechanism of AD pathogenesis and prevention.},
\r\nkeywords = {},
\r\npubstate = {published},
\r\ntppubtype = {article}
\r\n}
\r\n<\/pre><\/div>
Close<\/a><\/p><\/div>
Abstract Background Although amyloid deposition in brain is one of the hallmark pathological features of Alzheimer\u2019s disease (AD), the upstream events and its molecular environment in AD brain remain largely unknown. Recent advances in analytical methods such as mass spectrometry can provide the cutting-edge tools to unveil the AD pathogenesis at molecular and atomic level. Method In order to gain the comprehensive information about AD pathology at molecular level, postmortem brain sections of AD patients were analyzed by the hybrid molecular imaging methods composed of the conventional histological analyses, matrix assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) for small molecules, laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) imaging for metals, and particle induced X-ray emission (PIXE) imaging for elements. Principal components analysis (PCA) was also applied to discover the differences between the spectra datasets of AD and healthy control brains. Specific distribution of molecules and elements were visualized on the brain slice. (Ethics Approval: Dokkyo Medical University No.2021-004, Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology No.R20-003). Result MALDI-MSI showed abnormal distribution of lipids, such as phospholipids, sphingolipids, and their derivatives in AD brain. LA-ICP-MS visualized the distribution of elements, including some metals in the wide-field brain section. PIXE imaging displayed the specific concentration of atoms nearby the pathological tangles detected by anti-ubiquitin antibody. PCA proposed the effective classification to distinguish AD from control based on mass spectra. Conclusion Our data suggest the molecular and atomic environment in brain that is closely linked to AD pathology. Further analyses should be conducted to elucidate the fundamental mechanism of AD pathogenesis and prevention.<\/div>
Close<\/a><\/p><\/div>
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2023<\/h3>\r\n <\/td>\r\n <\/tr>

Miki, Yuta; Murakami, Hiroya; Gotoh, Marin; Umemura, Tomonari; Esaka, Yukihiro; Inoue, Yoshinori; Teshima, Norio<\/p>

Novel chemically cross-linked self-molding particulate sorbents as solid-phase extraction media<\/a> Journal Article<\/span> <\/p>

In: <\/span>Anal Sci, <\/span>vol. 39, <\/span>no. 5, <\/span>pp. 749\u2013754, <\/span>2023<\/span>, ISSN: 1348-2246<\/span>.<\/p>

Abstract<\/a><\/span> | Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

@article{pmid36001293,
\r\ntitle = {Novel chemically cross-linked self-molding particulate sorbents as solid-phase extraction media},
\r\nauthor = {Yuta Miki and Hiroya Murakami and Marin Gotoh and Tomonari Umemura and Yukihiro Esaka and Yoshinori Inoue and Norio Teshima},
\r\ndoi = {10.1007\/s44211-022-00179-3},
\r\nissn = {1348-2246},
\r\nyear  = {2023},
\r\ndate = {2023-05-01},
\r\njournal = {Anal Sci},
\r\nvolume = {39},
\r\nnumber = {5},
\r\npages = {749--754},
\r\nabstract = {Here, we describe novel, chemically cross-linked, self-molding particulate polymer sorbents that are utilized as a molding-type solid-phase extraction medium (M-SPEM), which exhibits high permeability and rigidness. To fabricate such M-SPEM, first, polyethyleneimine (PEI)-modified reversed-phase (RP)-type particulate sorbents were synthesized, thereafter, they were chemically cross-linked by a polymer having many epoxy groups together with additional PEI. By optimizing the binding conditions of the particulate sorbents, the resultant M-SPEM has almost the same adsorption properties as the corresponding unmolded particulate sorbent for some polar (e.g., uracil and adenine) compounds. The binding technique proposed here is expected to facilitate the fabrication of molding-type sorbents and improve the performance of the SPE procedure.},
\r\nkeywords = {},
\r\npubstate = {published},
\r\ntppubtype = {article}
\r\n}
\r\n<\/pre><\/div>
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Murakami, Hiroya; Iida, Keisuke; Oda, Yuki; Umemura, Tomonari; Nakajima, Hizuru; Esaka, Yukihiro; Inoue, Yoshinori; Teshima, Norio<\/p>

Hydrophilic interaction chromatography-type sorbent prepared by the modification of methacrylate-base resin with polyethyleneimine for solid-phase extraction of polar compounds<\/a> Journal Article<\/span> <\/p>

In: <\/span>Anal Sci, <\/span>vol. 39, <\/span>no. 3, <\/span>pp. 375\u2013381, <\/span>2023<\/span>, ISSN: 1348-2246<\/span>.<\/p>

Abstract<\/a><\/span> | Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

@article{pmid36577893,
\r\ntitle = {Hydrophilic interaction chromatography-type sorbent prepared by the modification of methacrylate-base resin with polyethyleneimine for solid-phase extraction of polar compounds},
\r\nauthor = {Hiroya Murakami and Keisuke Iida and Yuki Oda and Tomonari Umemura and Hizuru Nakajima and Yukihiro Esaka and Yoshinori Inoue and Norio Teshima},
\r\ndoi = {10.1007\/s44211-022-00250-z},
\r\nissn = {1348-2246},
\r\nyear  = {2023},
\r\ndate = {2023-03-01},
\r\njournal = {Anal Sci},
\r\nvolume = {39},
\r\nnumber = {3},
\r\npages = {375--381},
\r\nabstract = {Hydrophilic interaction chromatography (HILIC)-type sorbents were newly developed for the solid-phase extraction (SPE) of polar compounds. Two methacrylate-base resins with different cross-linking monomers and pore properties were synthesized, and three polyethyleneimines (PEIs) with different molecular weights were modified onto each base resin. In both cases, PEIs with a molecular weight of 10,000 (PEI-10,000) exhibited the highest adsorption properties for polar compounds (uracil, uridine, adenosine, cytidine, and guanosine). To control the water-enriched layer at the surface of the PEI-10,000-modified sorbents, the additive amount of PEI-10,000 in the modified reaction was also optimized. When 10 times the amount of PEI-10,000 to each base resin was added, an improvement in adsorption property was observed. Moreover, the use of a nonaqueous sample solution (100% acetonitrile) during the sample loading process drastically improved adsorption, especially for uracil (about 80%) and adenosine (100%). These results indicate that the formation of a strong water-enriched layer at the surface of sorbents with an effective expression of hydrophilic interaction was an important factor in the adsorption properties of polar compounds in HILIC mode-SPE.},
\r\nkeywords = {},
\r\npubstate = {published},
\r\ntppubtype = {article}
\r\n}
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Uchida, Masao; Mantoku, Kanako; Kumata, Hidetoshi; Kaneyasu, Naoki; Handa, Daishi; Arakaki, Takemitsu; Kobayashi, Toshiyuki; Hatakeyama, Shiro; Shibata, Yasuyuki; Kawamura, Kimitaka<\/p>

Source apportionment of black carbon aerosols by isotopes (14C and 13C) and Bayesian modeling from two remote islands in east Asian outflow region Journal Article<\/span> <\/p>

In: <\/span>Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, <\/span>vol. 538, <\/span>pp. 64\u201374, <\/span>2023<\/span>.<\/p>

BibTeX<\/a><\/span><\/p>

\u7530\u4e2d, \u674f\u5948; \u843d\u5408, \u967d\u9999; \u718a\u7530, \u82f1\u5cf0; \u698e\u672c, \u525b\u53f8; \u6885\u6751, \u77e5\u4e5f<\/p>

\u30a2\u30df\u30ce\u9178\u306e\u30da\u30f3\u30bf\u30d5\u30eb\u30aa\u30ed\u30d9\u30f3\u30b8\u30eb\u8a98\u5c0e\u4f53\u306eGC\/EI-MS\u306b\u3088\u308b\u89e3\u6790<\/a> Journal Article<\/span> <\/p>

In: <\/span>\u5206\u6790\u5316\u5b66, <\/span>vol. 72, <\/span>no. 10.11, <\/span>pp. 407-416, <\/span>2023<\/span>.<\/p>

Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

\r\n

2022<\/h3>\r\n <\/td>\r\n <\/tr>

Shoji, Atsushi; Nakajima, Miyu; Morioka, Kazuhiro; Fujimori, Eiji; Umemura, Tomonari; Yanagida, Akio; Hemmi, Akihide; Uchiyama, Katsumi; Nakajima, Hizuru<\/p>

Development of a surface plasmon resonance sensor using an optical fiber prepared by electroless displacement gold plating and its application to immunoassay<\/a> Journal Article<\/span> <\/p>

In: <\/span>Talanta, <\/span>vol. 240, <\/span>pp. 123162, <\/span>2022<\/span>, ISSN: 1873-3573<\/span>.<\/p>

Abstract<\/a><\/span> | Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

@article{pmid34996015,
\r\ntitle = {Development of a surface plasmon resonance sensor using an optical fiber prepared by electroless displacement gold plating and its application to immunoassay},
\r\nauthor = {Atsushi Shoji and Miyu Nakajima and Kazuhiro Morioka and Eiji Fujimori and Tomonari Umemura and Akio Yanagida and Akihide Hemmi and Katsumi Uchiyama and Hizuru Nakajima},
\r\ndoi = {10.1016\/j.talanta.2021.123162},
\r\nissn = {1873-3573},
\r\nyear  = {2022},
\r\ndate = {2022-04-01},
\r\njournal = {Talanta},
\r\nvolume = {240},
\r\npages = {123162},
\r\nabstract = {A simple and low-cost method of fabricating an optical fiber for a surface plasmon resonance (SPR) sensor was proposed. The method is based on the electroless nickel plating and subsequent displacement gold plating of the core of the optical fiber. The thickness of the nickel and gold thin films deposited on the core of the optical fiber could be controlled by measuring the reflected light intensity from the tip of the optical fiber during the plating processes. The sensitivity and resolution of the SPR sensor with the fabricated optical fiber in the refractive index range from 1.333 to 1.348 were 1324.3 nm\/RIU and 7.6\u00a0\u00d7\u00a010 RIU, respectively. The developed SPR sensor was successfully used in the determination of immunoglobulin A (IgA) in human saliva. The IgA quantification results obtained by the SPR sensor were in excellent agreement with those obtained by conventional enzyme-linked immunosorbent assay using a 96-well microtiter plate.},
\r\nkeywords = {},
\r\npubstate = {published},
\r\ntppubtype = {article}
\r\n}
\r\n<\/pre><\/div>
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Miki, Yuta; Murakami, Hiroya; Iida, Keisuke; Umemura, Tomonari; Esaka, Yukihiro; Inoue, Yoshinori; Teshima, Norio<\/p>

Preparation and evaluation of molding-type solid-phase extraction media binding with commercially available adhesives<\/a> Journal Article<\/span> <\/p>

In: <\/span>Anal Sci, <\/span>vol. 38, <\/span>no. 2, <\/span>pp. 307\u2013315, <\/span>2022<\/span>, ISSN: 1348-2246<\/span>.<\/p>

Abstract<\/a><\/span> | Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

@article{pmid35314976,
\r\ntitle = {Preparation and evaluation of molding-type solid-phase extraction media binding with commercially available adhesives},
\r\nauthor = {Yuta Miki and Hiroya Murakami and Keisuke Iida and Tomonari Umemura and Yukihiro Esaka and Yoshinori Inoue and Norio Teshima},
\r\ndoi = {10.2116\/analsci.21P265},
\r\nissn = {1348-2246},
\r\nyear  = {2022},
\r\ndate = {2022-02-01},
\r\njournal = {Anal Sci},
\r\nvolume = {38},
\r\nnumber = {2},
\r\npages = {307--315},
\r\nabstract = {A fabrication method of molding-type solid-phase extraction media (M-SPEM) bound with commercially available adhesive is presented. Six pieces of M-SPEM were prepared by heating each kneaded product of a particulate sorbent and an adhesive inserted into a six-hole cylindrical mold for hardening under an open system and normal pressure. The particulate sorbent contained in M-SPEM was divinylbenzene-based reversed-phase mode solid-phase extractants that we have reported. An examination of several adhesives showed that the moldability of M-SPEM depended on the composition and properties of the adhesive. The optimized procedure can be used to prepare an M-SPEM containing an 85 wt% particulate sorbent (particulate sorbent\/adhesive, 100\u00a0mg\/17\u00a0mg; particle diameter, 90-150\u00a0\u00b5m), and the M-SPEM has a specific surface area of about 500\u00a0m\/g. The established procedure in this study can bind particulate sorbents together, which showed almost no reductions in the adsorption property and liquid permeability compared with those of the particulate sorbent.},
\r\nkeywords = {},
\r\npubstate = {published},
\r\ntppubtype = {article}
\r\n}
\r\n<\/pre><\/div>
Close<\/a><\/p><\/div>

Ishii, Ryo; Morioka, Kazuhiro; Mizumoto, Takuya; Yamasaki, Natsumi; Hemmi, Akihide; Shoji, Atsushi; Murakami, Hiroya; Teshima, Norio; Umemura, Tomonari; Uchiyama, Katsumi; Nakajima, Hizuru<\/p>

Development of Portable Fluorescence Microplate Reader Equipped with Indium Tin Oxide Glass Heater for Loop-mediated Isothermal Amplification<\/a> Journal Article<\/span> <\/p>

In: <\/span>Sensors and Materials, <\/span>vol. 34, <\/span>pp. 1, <\/span>2022<\/span>.<\/p>

Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

\r\n

2021<\/h3>\r\n <\/td>\r\n <\/tr>

Miyauchi, Hiroki; Harada, Kohei; Suzuki, Yoshino; Okada, Katsuhiko; Aoki, Motohide; Umemura, Tomonari; Fujiwara, Shoko; Tsuzuki, Mikio<\/p>

Development of an algal cell-attached solid surface culture system for simultaneous wastewater treatment and biomass production<\/a> Journal Article<\/span> <\/p>

In: <\/span>Algal Research, <\/span>vol. 58, <\/span>pp. 102394, <\/span>2021<\/span>, ISSN: 2211-9264<\/span>.<\/p>

Abstract<\/a><\/span> | Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

@article{MIYAUCHI2021102394,
\r\ntitle = {Development of an algal cell-attached solid surface culture system for simultaneous wastewater treatment and biomass production},
\r\nauthor = {Hiroki Miyauchi and Kohei Harada and Yoshino Suzuki and Katsuhiko Okada and Motohide Aoki and Tomonari Umemura and Shoko Fujiwara and Mikio Tsuzuki},
\r\nurl = {https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2211926421002137},
\r\ndoi = {https:\/\/doi.org\/10.1016\/j.algal.2021.102394},
\r\nissn = {2211-9264},
\r\nyear  = {2021},
\r\ndate = {2021-07-06},
\r\njournal = {Algal Research},
\r\nvolume = {58},
\r\npages = {102394},
\r\nabstract = {Wastewater treatment using microalgae is receiving growing attention. Here, we have developed a portable tubular system containing an algal cell-coated solid surface for phosphorous recovery. P-depleted Chlorella cells attached to a solid surface removed phosphate from the medium about 70 times faster than P-replete cells. When the cell density was 20\u00a0g\u00a0dry\u00a0cell\u00a0weight\u00a0m\u22122 or less, P-depleted cells on the solid surface absorbed phosphate from the medium at almost the same rate as in liquid, the maximum capacity per solid surface area being about 6\u00a0mg\u00a0P\u00a0m\u22122\u00a0min\u22121. P in inorganic wastewater from chemical factories (ethanol factories; about 4\u00a0mg\u00a0L\u22121) and in pond water (0.06\u00a0mg\u00a0L\u22121) was mostly removed within 3\u00a0h with a simple device composed of a single solid-surfaced sheet (0.002\u00a0m\u22122) and a portable compact tubular device including 12 such sheets (totally 0.5\u00a0m\u22122), respectively. Simultaneously, cellular growth was confirmed with both wastewater and pond water. These findings suggested that the attached cell culture system, in which medium exchange to prepare P-depleted cells is much easier than in liquid cultures, is promising for dual use for biomass production and wastewater treatment.},
\r\nkeywords = {},
\r\npubstate = {published},
\r\ntppubtype = {article}
\r\n}
\r\n<\/pre><\/div>
Close<\/a><\/p><\/div>
Wastewater treatment using microalgae is receiving growing attention. Here, we have developed a portable tubular system containing an algal cell-coated solid surface for phosphorous recovery. P-depleted Chlorella cells attached to a solid surface removed phosphate from the medium about 70 times faster than P-replete cells. When the cell density was 20\u00a0g\u00a0dry\u00a0cell\u00a0weight\u00a0m\u22122 or less, P-depleted cells on the solid surface absorbed phosphate from the medium at almost the same rate as in liquid, the maximum capacity per solid surface area being about 6\u00a0mg\u00a0P\u00a0m\u22122\u00a0min\u22121. P in inorganic wastewater from chemical factories (ethanol factories; about 4\u00a0mg\u00a0L\u22121) and in pond water (0.06\u00a0mg\u00a0L\u22121) was mostly removed within 3\u00a0h with a simple device composed of a single solid-surfaced sheet (0.002\u00a0m\u22122) and a portable compact tubular device including 12 such sheets (totally 0.5\u00a0m\u22122), respectively. Simultaneously, cellular growth was confirmed with both wastewater and pond water. These findings suggested that the attached cell culture system, in which medium exchange to prepare P-depleted cells is much easier than in liquid cultures, is promising for dual use for biomass production and wastewater treatment.<\/div>
Close<\/a><\/p><\/div>

MIKI, Yuta; MURAKAMI, Hiroya; IIDA, Keisuke; UMEMURA, Tomonari; ESAKA, Yukihiro; INOUE, Yoshinori; TESHIMA, Norio<\/p>

Preparation and Evaluation of Molding-type Solid-phase Extraction Media Binding with Commercially Available Adhesives<\/a> Journal Article<\/span> <\/p>

In: <\/span>Analytical Sciences, <\/span>2021<\/span>.<\/p>

Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

Morioka, Kazuhiro; Osashima, Moeko; Azuma, Nao; Qu, Kuizhi; Hemmi, Akihide; Shoji, Atsushi; Murakami, Hiroya; Teshima, Norio; Umemura, Tomonari; Uchiyama, Katsumi; Nakajima, Hizuru<\/p>

Development of a fluorescence microplate reader using an organic photodiode array with a large light receiving area<\/a> Journal Article<\/span> <\/p>

In: <\/span>Talanta, <\/span>vol. 238, <\/span>pp. 122994, <\/span>2021<\/span>.<\/p>

Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

\r\n

2020<\/h3>\r\n <\/td>\r\n <\/tr>

Matsuo, Yukiko; Sasaki, Mayu; Fukaya, Haruhiko; Miyake, Katsunori; Takeuchi, Riko; Kumata, Hidetoshi; Mimaki, Yoshihiro<\/p>

Chemical Constituents of the Terrestrial Stems of Ephedra sinica and their PPAR-&gamma; Ligand-Binding Activity<\/a> Journal Article<\/span> <\/p>

In: <\/span>Planta Medica International Open, <\/span>vol. 7, <\/span>no. 01, <\/span>pp. e12-e16, <\/span>2020<\/span>, ISSN: 2509-9264<\/span>, (e12)<\/span>.<\/p>

Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

Wakana, Yuichi; Hayashi, Kaito; Nemoto, Takumi; Watanabe, Chiaki; Taoka, Masato; Angulo-Capel, Jessica; Parajo, Maria; Kumata, Hidetoshi; Umemura, Tomonari; Inoue, Hiroki; Arasaki, Kohei; Campelo, Felix; Tagaya, Mitsuo<\/p>

The ER cholesterol sensor SCAP promotes CARTS biogenesis at ER\u2013Golgi membrane contact sites<\/a> Journal Article<\/span> <\/p>

In: <\/span>Journal of Cell Biology, <\/span>vol. 220, <\/span>2020<\/span>.<\/p>

Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

\u4eca\u5d0e, \u9f8d\u4e4b\u4ecb; \u8fd1\u85e4, \u5553\u592a; \u8c37, \u590f\u6d77; \u9752\u6728, \u5143\u79c0; \u718a\u7530, \u82f1\u5cf0; \u5185\u7530, \u9054\u4e5f; \u9577\u7e04, \u8c6a; \u5d8b\u7530, \u6cf0\u4f51; \u7530\u53e3, \u5609\u5f66; \u4f50\u85e4, \u6d69\u660e; \u5b89\u4e95, \u9686\u96c4; \u6885\u6751, \u77e5\u4e5f<\/p>

\u8d85\u8584\u5c64\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u7528\u306e\u6c34\u5e73\u5f0f\u30df\u30cb\u30c1\u30e5\u30a2TLC\u5c55\u958b\u69fd\u306e\u8a66\u4f5c\u3068\u8a55\u4fa1<\/a> Journal Article<\/span> <\/p>

In: <\/span>\u5206\u6790\u5316\u5b66, <\/span>vol. 69, <\/span>no. 10.11, <\/span>pp. 553-558, <\/span>2020<\/span>.<\/p>

Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

Kumata, Hidetoshi; Morimoto, Chika; Horie, Akihiro; Tanabe, Akane; Fujimori, Eiji; Umemura, Tomonari<\/p>

Elimination of interfering molybdenum oxyanion with an anion-exchange monolithic spin tip (AXTip) for precise determination of cadmium in human urine by ICP-MS<\/a> Journal Article<\/span> <\/p>

In: <\/span>Talanta Open, <\/span>pp. 100009, <\/span>2020<\/span>, ISSN: 2666-8319<\/span>.<\/p>

Abstract<\/a><\/span> | Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

@article{KUMATA2020100009,
\r\ntitle = {Elimination of interfering molybdenum oxyanion with an anion-exchange monolithic spin tip (AXTip) for precise determination of cadmium in human urine by ICP-MS},
\r\nauthor = {Hidetoshi Kumata and Chika Morimoto and Akihiro Horie and Akane Tanabe and Eiji Fujimori and Tomonari Umemura},
\r\nurl = {http:\/\/www.sciencedirect.com\/science\/article\/pii\/S2666831920300096},
\r\ndoi = {https:\/\/doi.org\/10.1016\/j.talo.2020.100009},
\r\nissn = {2666-8319},
\r\nyear  = {2020},
\r\ndate = {2020-01-01},
\r\njournal = {Talanta Open},
\r\npages = {100009},
\r\nabstract = {A simple purification technique using an anion-exchange monolithic spin tip was described for the elimination of molybdenum oxyanion interfering with precise determination of cadmium in human urine samples by ICP-MS. The tip device named \u201canion-exchange tip-in monolith (AXTip)\u201d was laboratory-prepared within the confines of a commercially available syringe filter tip by a two-step process: (1) in situ copolymerization of glycidyl methacrylate (GMA) and ethylene dimethacrylate (EDMA) and (2) its subsequent modification with trimethylamine (TMA), as a strong anion-exchange functional group, via ring-opening reaction of epoxide. The adsorption capacity of the AXTip was >3.2 \u03bcmol\/tip for molybdenum oxyanion. The performance of the AXTip was evaluated through an adsorption\/non-adsorption experiment for molybdenum, cadmium and other metal and metalloid species, using a model solution and human urine samples to optimize the sample solution's pH and dilution factor for the AXTip treatment. In the case of urine sample analysis, 8 times dilution and pH adjustment to 4.0 prior to AXTip treatment were found to allow >98 % elimination of interfering molybdenum oxyanions and quantitative recovery (103\u00b17 %) of cadmium. At the optimized conditions, the AXTip treatment reduces Mo\/Cd ratio from \u223c400 in human urines to <10, which is sufficient for conducting ICP-MS measurement of cadmium in no-gas mode (without collision\/reaction cell technique). The proposed purification technique was validated through the analysis of an accuracy control material of human urine (Seronorm\u2122 Trace Elements Urine L-1). Good agreement of the observed values with the reference values indicates that the proposed technique is practically applicable.},
\r\nkeywords = {},
\r\npubstate = {published},
\r\ntppubtype = {article}
\r\n}
\r\n<\/pre><\/div>
Close<\/a><\/p><\/div>
A simple purification technique using an anion-exchange monolithic spin tip was described for the elimination of molybdenum oxyanion interfering with precise determination of cadmium in human urine samples by ICP-MS. The tip device named \u201canion-exchange tip-in monolith (AXTip)\u201d was laboratory-prepared within the confines of a commercially available syringe filter tip by a two-step process: (1) in situ copolymerization of glycidyl methacrylate (GMA) and ethylene dimethacrylate (EDMA) and (2) its subsequent modification with trimethylamine (TMA), as a strong anion-exchange functional group, via ring-opening reaction of epoxide. The adsorption capacity of the AXTip was >3.2 \u03bcmol\/tip for molybdenum oxyanion. The performance of the AXTip was evaluated through an adsorption\/non-adsorption experiment for molybdenum, cadmium and other metal and metalloid species, using a model solution and human urine samples to optimize the sample solution's pH and dilution factor for the AXTip treatment. In the case of urine sample analysis, 8 times dilution and pH adjustment to 4.0 prior to AXTip treatment were found to allow >98 % elimination of interfering molybdenum oxyanions and quantitative recovery (103\u00b17 %) of cadmium. At the optimized conditions, the AXTip treatment reduces Mo\/Cd ratio from \u223c400 in human urines to <10, which is sufficient for conducting ICP-MS measurement of cadmium in no-gas mode (without collision\/reaction cell technique). The proposed purification technique was validated through the analysis of an accuracy control material of human urine (Seronorm\u2122 Trace Elements Urine L-1). Good agreement of the observed values with the reference values indicates that the proposed technique is practically applicable.<\/div>
Close<\/a><\/p><\/div>

Murakami, Hiroya; Omiya, Miho; Miki, Yuta; Umemura, Tomonari; Esaka, Yukihiro; Inoue, Yoshinori; Teshima, Norio<\/p>

Evaluation of the adsorption properties of nucleobase-modified sorbents for a solid-phase extraction of water-soluble compounds<\/a> Journal Article<\/span> <\/p>

In: <\/span>Talanta, <\/span>vol. 217, <\/span>pp. 121052, <\/span>2020<\/span>, ISSN: 0039-9140<\/span>.<\/p>

Abstract<\/a><\/span> | Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

@article{MURAKAMI2020121052,
\r\ntitle = {Evaluation of the adsorption properties of nucleobase-modified sorbents for a solid-phase extraction of water-soluble compounds},
\r\nauthor = {Hiroya Murakami and Miho Omiya and Yuta Miki and Tomonari Umemura and Yukihiro Esaka and Yoshinori Inoue and Norio Teshima},
\r\nurl = {http:\/\/www.sciencedirect.com\/science\/article\/pii\/S003991402030343X},
\r\ndoi = {https:\/\/doi.org\/10.1016\/j.talanta.2020.121052},
\r\nissn = {0039-9140},
\r\nyear  = {2020},
\r\ndate = {2020-01-01},
\r\njournal = {Talanta},
\r\nvolume = {217},
\r\npages = {121052},
\r\nabstract = {We developed hydrophilic interaction chromatography (HILIC)-type sorbents modified with nucleobases for solid phase extraction (SPE). The synthesized hydrophilic base resins were modified by each nucleobase (adenine, guanine, and cytosine). The measurement of the amount of water content indicated that each nucleobase-modified sorbent had a water layer. To evaluate the adsorption properties in the HILIC mode, we chose two nucleobases (uracil and adenine) and four nucleosides (uridine, adenosine, cytidine, guanosine) as water-soluble analytes, which were loaded into an SPE cartridge packed with the nucleobase-modified sorbent. Firstly, 95% acetonitrile (ACN) solutions were used in the process of conditioning and sample loading of the above polar analytes. High recoveries of the analytes were observed in each nucleobase-modified sorbent, and the Diol-type sorbent (no modification with any of the nucleobases) did not adsorb each water-soluble analyte. On the basis of this result, a 98% ACN solution was used during the process of conditioning and sample loading to decrease the concentration of water in the sample, which potentially inhibited the formation of hydrogen bonding between each analyte and the modified nucleobase. Considerable improvements of recoveries were observed in Adenine- and Cytosine-modified sorbents. These results were possibly attributed to the effective expression of hydrogen bonding by decreasing water concentration in the sample solution. Although a non-aqueous (100% ACN) sample solution can be expected to obtain higher recoveries compared with the 98% ACN solution, a decrease in recoveries was observed in Adenine-modified sorbent. From these results, the highest adsorption property was observed in Adenine-modified sorbent using 98% ACN as a sample condition, and the combination of this sample condition and sorbent is effective for high adsorption under HILIC condition. Moreover, we also revealed that a balance between the thickness of water layer and the modification amount of nucleobase is important for retention in the HILIC-type sorbent.},
\r\nkeywords = {},
\r\npubstate = {published},
\r\ntppubtype = {article}
\r\n}
\r\n<\/pre><\/div>
Close<\/a><\/p><\/div>
We developed hydrophilic interaction chromatography (HILIC)-type sorbents modified with nucleobases for solid phase extraction (SPE). The synthesized hydrophilic base resins were modified by each nucleobase (adenine, guanine, and cytosine). The measurement of the amount of water content indicated that each nucleobase-modified sorbent had a water layer. To evaluate the adsorption properties in the HILIC mode, we chose two nucleobases (uracil and adenine) and four nucleosides (uridine, adenosine, cytidine, guanosine) as water-soluble analytes, which were loaded into an SPE cartridge packed with the nucleobase-modified sorbent. Firstly, 95% acetonitrile (ACN) solutions were used in the process of conditioning and sample loading of the above polar analytes. High recoveries of the analytes were observed in each nucleobase-modified sorbent, and the Diol-type sorbent (no modification with any of the nucleobases) did not adsorb each water-soluble analyte. On the basis of this result, a 98% ACN solution was used during the process of conditioning and sample loading to decrease the concentration of water in the sample, which potentially inhibited the formation of hydrogen bonding between each analyte and the modified nucleobase. Considerable improvements of recoveries were observed in Adenine- and Cytosine-modified sorbents. These results were possibly attributed to the effective expression of hydrogen bonding by decreasing water concentration in the sample solution. Although a non-aqueous (100% ACN) sample solution can be expected to obtain higher recoveries compared with the 98% ACN solution, a decrease in recoveries was observed in Adenine-modified sorbent. From these results, the highest adsorption property was observed in Adenine-modified sorbent using 98% ACN as a sample condition, and the combination of this sample condition and sorbent is effective for high adsorption under HILIC condition. Moreover, we also revealed that a balance between the thickness of water layer and the modification amount of nucleobase is important for retention in the HILIC-type sorbent.<\/div>
Close<\/a><\/p><\/div>

\u66f2, \u594e\u667a; \u68ee\u5ca1, \u548c\u5927; \u6771, \u5948\u7a57; \u9577\u5d8b, \u840c\u5b50; \u8fba\u898b, \u5f70\u79c0; \u6771\u6d77\u6797, \u6566; \u6751\u4e0a, \u535a\u54c9; \u624b\u5d8b, \u7d00\u96c4; \u6885\u6751, \u77e5\u4e5f; \u52a0\u85e4, \u4fca\u543e; \u6cb3\u897f, \u5948\u4fdd\u5b50; \u5185\u5c71, \u4e00\u7f8e; \u4e2d\u5d8b, \u79c0<\/p>

\u81ea\u5f8b\u9001\u6db2\u304c\u53ef\u80fd\u306a\u30de\u30a4\u30af\u30ed\u30c1\u30c3\u30d7\u3068\u6709\u6a5f\u30d5\u30a9\u30c8\u30c0\u30a4\u30aa\u30fc\u30c9\u691c\u51fa\u5668\u3092\u7528\u3044\u308b\u5316\u5b66\u767a\u5149\u5206\u6790\u30b7\u30b9\u30c6\u30e0\u306e\u958b\u767a<\/a> Journal Article<\/span> <\/p>

In: <\/span>\u5206\u6790\u5316\u5b66, <\/span>vol. 69, <\/span>no. 1.2, <\/span>pp. 31-39, <\/span>2020<\/span>.<\/p>

Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

Endo, Satoshi; Yoshimura, Mitsuki; Kumata, Hidetoshi; Uchida, Masao; Yabuki, Yoshinori; Nakata, Haruhiko<\/p>

Reduced bioavailability of polycyclic aromatic hydrocarbons (PAHs) in sediments impacted by carbon manufacturing plant effluent: Evaluation by ex situ passive sampling method<\/a> Journal Article<\/span> <\/p>

In: <\/span>Environmental Pollution, <\/span>vol. 256, <\/span>pp. 113448, <\/span>2020<\/span>, ISSN: 0269-7491<\/span>.<\/p>

Abstract<\/a><\/span> | Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

@article{ENDO2020113448,
\r\ntitle = {Reduced bioavailability of polycyclic aromatic hydrocarbons (PAHs) in sediments impacted by carbon manufacturing plant effluent: Evaluation by ex situ passive sampling method},
\r\nauthor = {Satoshi Endo and Mitsuki Yoshimura and Hidetoshi Kumata and Masao Uchida and Yoshinori Yabuki and Haruhiko Nakata},
\r\nurl = {http:\/\/www.sciencedirect.com\/science\/article\/pii\/S0269749119349796},
\r\ndoi = {https:\/\/doi.org\/10.1016\/j.envpol.2019.113448},
\r\nissn = {0269-7491},
\r\nyear  = {2020},
\r\ndate = {2020-01-01},
\r\njournal = {Environmental Pollution},
\r\nvolume = {256},
\r\npages = {113448},
\r\nabstract = {Potential risks of polycyclic aromatic hydrocarbons (PAHs) in sediments of a Japanese bay contaminated by carbon manufacturing plant effluent were evaluated by calculating toxicity units (TUs). TUs calculated from the measured whole-sediment concentrations (Cwhole) were often higher than or close to 1, signaling a possible toxicity concern to benthic organisms. In contrast, TUs based on the freely dissolved pore water concentrations (Cfree) measured by an ex-situ passive sampling method with polyethylene strips were 0.0007\u20130.005, much lower than 1, indicating no effect. We also found that the fractions of black carbon in sediments of the contaminated bay were significantly higher than those of reference sites. Overall, we conclude that carbon manufacturing plant effluent substantially increases Cwhole of PAHs in sediments but also increases the fraction of carbonaceous particles that strongly retain PAHs. As a combined result, bioavailable concentrations (as expressed by pore water Cfree) of PAHs do not increase as much as Cwhole. The results of this study indicate that ecotoxicological risks of PAH contamination by carbon manufacturing plants should be evaluated by directly measuring pore water Cfree instead of Cwhole.},
\r\nkeywords = {},
\r\npubstate = {published},
\r\ntppubtype = {article}
\r\n}
\r\n<\/pre><\/div>
Close<\/a><\/p><\/div>

Kishi, Takuho; Kotani, Akira; Umemura, Tomonari; Hakamata, Hideki<\/p>

HPLC with electrochemical detection for determining homogentisic acid and its application to urine from rats fed tyrosine-enriched food<\/a> Journal Article<\/span> <\/p>

In: <\/span>Journal of Pharmaceutical and Biomedical Analysis, <\/span>vol. 186, <\/span>pp. 113253, <\/span>2020<\/span>, ISSN: 0731-7085<\/span>.<\/p>

Abstract<\/a><\/span> | Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

@article{KISHI2020113253,
\r\ntitle = {HPLC with electrochemical detection for determining homogentisic acid and its application to urine from rats fed tyrosine-enriched food},
\r\nauthor = {Takuho Kishi and Akira Kotani and Tomonari Umemura and Hideki Hakamata},
\r\nurl = {http:\/\/www.sciencedirect.com\/science\/article\/pii\/S073170851932967X},
\r\ndoi = {https:\/\/doi.org\/10.1016\/j.jpba.2020.113253},
\r\nissn = {0731-7085},
\r\nyear  = {2020},
\r\ndate = {2020-01-01},
\r\njournal = {Journal of Pharmaceutical and Biomedical Analysis},
\r\nvolume = {186},
\r\npages = {113253},
\r\nabstract = {A highly sensitive method for determining urine homogentisic acid (HGA) is required to provide adequate diagnosis and therapy for alkaptonuria in early stages. In this study, we developed a highly sensitive high-performance liquid chromatography with electrochemical detection (HPLC-ECD) for determining HGA in urine. In order to obtain a chromatogram of HGA by HPLC-ECD, an oxidation current was monitored at +0.5\u202fV vs. Ag\/AgCl. The peak heights of HGA showed linearity (r\u202f=\u202f0.999) ranging from 4.2\u202fng\/mL to 168\u202fng\/mL, and the detection limit was 1.2\u202fng\/mL (signal-to-noise ratio, S\/N\u202f=\u202f3). In recovery tests using human control urine spiked with an HGA standard, the recoveries of HGA were more than 93.2 %, and the relative standard deviations (n\u202f=\u202f6) were less than 1.9 %. As an in vivo application using male Wistar rats, the level of urine HGA, which was metabolized from tyrosine in tyrosine-enriched food, was determined by this HPLC-ECD method. The determination of HGA in urine by this HPLC-ECD method requires only 0.1\u202fmL of a rat urine specimen and simple sample preparation consisting of dilution and filtration.},
\r\nkeywords = {},
\r\npubstate = {published},
\r\ntppubtype = {article}
\r\n}
\r\n<\/pre><\/div>
Close<\/a><\/p><\/div>
\r\n

2019<\/h3>\r\n <\/td>\r\n <\/tr>

Tani, Hidenori; Numajiri, Ayaka; Aoki, Motohide; Umemura, Tomonari; Nakazato, Tetsuya<\/p>

Short-lived long noncoding RNAs as surrogate indicators for chemical stress in HepG2 cells and their degradation by nuclear RNases<\/a> Journal Article<\/span> <\/p>

In: <\/span>Scientific Reports, <\/span>vol. 9, <\/span>pp. 20299, <\/span>2019<\/span>, ISSN: 2045-2322<\/span>.<\/p>

Abstract<\/a><\/span> | Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

@article{Tani2019,
\r\ntitle = {Short-lived long noncoding RNAs as surrogate indicators for chemical stress in HepG2 cells and their degradation by nuclear RNases},
\r\nauthor = {Hidenori Tani and Ayaka Numajiri and Motohide Aoki and Tomonari Umemura and Tetsuya Nakazato},
\r\nurl = {www.nature.com\/scientificreports},
\r\ndoi = {https:\/\/doi.org\/10.1038\/s41598-019-56869-y},
\r\nissn = {2045-2322},
\r\nyear  = {2019},
\r\ndate = {2019-12-30},
\r\njournal = {Scientific Reports},
\r\nvolume = {9},
\r\npages = {20299},
\r\nabstract = {Long noncoding RNAs (lncRNAs) are non-protein-coding transcripts >200 nucleotides in length that have been shown to play important roles in various biological processes. The mechanisms underlying the induction of lncRNA expression by chemical exposure remain to be determined. We identified a novel class of short-lived lncRNAs with half-lives (t1\/2) \u22644\u2009hours in human HeLa Tet-off cells, which have been suggested to express many lncRNAs with regulatory functions. As they may affect various human biological processes, short-lived lncRNAs may be useful indicators of the degree of stress on chemical exposure. In the present study, we identified four short-lived lncRNAs, designated as OIP5-AS1, FLJ46906, LINC01137, and GABPB1-AS1, which showed significantly upregulated expression following exposure to hydrogen peroxide (oxidative stress), mercury II chloride (heavy metal stress), and etoposide (DNA damage stress) in human HepG2 cells. These lncRNAs may be useful indicators of chemical stress responses. The levels of these lncRNAs in the cells were increased because of chemical stress-induced prolongation of their decay. These lncRNAs were degraded by nuclear RNases, which are components of the exosome and XRN2, and chemical exposure inhibited the RNase activities within the cells.},
\r\nkeywords = {},
\r\npubstate = {published},
\r\ntppubtype = {article}
\r\n}
\r\n<\/pre><\/div>
Close<\/a><\/p><\/div>

Umemura, Tomonari<\/p>

Elucidation of Ion-Pairing Elution Behavior of Anions and Cations in Electrostatic Ion Chromatography Using Water as a Mobile Phase Journal Article<\/span> <\/p>

In: <\/span>Chromatography, <\/span>vol. 40, <\/span>pp. 143-148, <\/span>2019<\/span>, ISSN: 1342-8284<\/span>.<\/p>

BibTeX<\/a><\/span><\/p>

\u7af9\u5185, \u7406\u5b50; \u529b\u77f3, \u5609\u4eba; \u5c0f\u5ddd, \u5948\u3005\u5b50; \u98a8\u5442\u7530, \u90f7\u53f2; \u5927\u6cb3\u5185, \u76f4\u5f66; \u9752\u6728, \u5143\u79c0; \u5185\u7530, \u9054\u4e5f; \u6885\u6751, \u77e5\u4e5f; \u718a\u7530, \u82f1\u5cf0<\/p>

\u5316\u5408\u7269\u30ec\u30d9\u30eb\u70ad\u7d20\u5b89\u5b9a\u540c\u4f4d\u4f53\u6bd4\u5206\u6790\u306e\u305f\u3081\u306e\u9ad8\u7b49\u690d\u7269\u4e2d\u306e\u30b9\u30c6\u30ed\u30fc\u30eb\u53ca\u3073\u8102\u80aa\u9178\u306e\u5206\u96e2\u7cbe\u88fd\u6cd5\u306e\u78ba\u7acb<\/a> Journal Article<\/span> <\/p>

In: <\/span>\u5206\u6790\u5316\u5b66, <\/span>vol. 68, <\/span>no. 5, <\/span>pp. 297-306, <\/span>2019<\/span>.<\/p>

Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

Nagashima, Shun; Takeda, Keisuke; Ohno, Nobuhiko; Ishido, Satoshi; Aoki, Motohide; Saitoh, Yurika; Takada, Takumi; Tokuyama, Takeshi; Sugiura, Ayumu; Fukuda, Toshifumi; Matsushita, Nobuko; Inatome, Ryoko; Yanagi, Shigeru<\/p>

MITOL deletion in the brain impairs mitochondrial structure and ER tethering leading to oxidative stress<\/a> Journal Article<\/span> <\/p>

In: <\/span>Life Science Alliance, <\/span>vol. 2, <\/span>no. 4, <\/span>2019<\/span>.<\/p>

Abstract<\/a><\/span> | Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

@article{Nagashimae201900308,
\r\ntitle = {MITOL deletion in the brain impairs mitochondrial structure and ER tethering leading to oxidative stress},
\r\nauthor = {Shun Nagashima and Keisuke Takeda and Nobuhiko Ohno and Satoshi Ishido and Motohide Aoki and Yurika Saitoh and Takumi Takada and Takeshi Tokuyama and Ayumu Sugiura and Toshifumi Fukuda and Nobuko Matsushita and Ryoko Inatome and Shigeru Yanagi},
\r\nurl = {https:\/\/www.life-science-alliance.org\/content\/2\/4\/e201900308},
\r\ndoi = {10.26508\/lsa.201900308},
\r\nyear  = {2019},
\r\ndate = {2019-01-01},
\r\njournal = {Life Science Alliance},
\r\nvolume = {2},
\r\nnumber = {4},
\r\npublisher = {Life Science Alliance},
\r\nabstract = {Mitochondrial abnormalities are associated with developmental disorders, although a causal relationship remains largely unknown. Here, we report that increased oxidative stress in neurons by deletion of mitochondrial ubiquitin ligase MITOL causes a potential neuroinflammation including aberrant astrogliosis and microglial activation, indicating that mitochondrial abnormalities might confer a risk for inflammatory diseases in brain such as psychiatric disorders. A role of MITOL in both mitochondrial dynamics and ER-mitochondria tethering prompted us to characterize three-dimensional structures of mitochondria in vivo. In MITOL-deficient neurons, we observed a significant reduction in the ER-mitochondria contact sites, which might lead to perturbation of phospholipids transfer, consequently reduce cardiolipin biogenesis. We also found that branched large mitochondria disappeared by deletion of MITOL. These morphological abnormalities of mitochondria resulted in enhanced oxidative stress in brain, which led to astrogliosis and microglial activation partly causing abnormal behavior. In conclusion, the reduced ER-mitochondria tethering and excessive mitochondrial fission may trigger neuroinflammation through oxidative stress.},
\r\nkeywords = {},
\r\npubstate = {published},
\r\ntppubtype = {article}
\r\n}
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Murota, Chisato; Fujiwara, Shoko; Tsujishita, Maki; Urabe, Kanae; Takayanagi, Shuta; Aoki, Motohide; Umemura, Tomonari; Eaton-Rye, Julian J; Pitt, Frances D; Tsuzuki, Mikio<\/p>

Hyper-resistance to arsenate in the cyanobacterium Synechocystis sp. PCC 6803 is influenced by the differential kinetics of its pst-ABC transporters and external phosphate concentration exposure<\/a> Journal Article<\/span> <\/p>

In: <\/span>Algal Research, <\/span>vol. 38, <\/span>pp. 101410, <\/span>2019<\/span>, ISSN: 2211-9264<\/span>.<\/p>

Abstract<\/a><\/span> | Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

@article{MUROTA2019101410,
\r\ntitle = {Hyper-resistance to arsenate in the cyanobacterium Synechocystis sp. PCC 6803 is influenced by the differential kinetics of its pst-ABC transporters and external phosphate concentration exposure},
\r\nauthor = {Chisato Murota and Shoko Fujiwara and Maki Tsujishita and Kanae Urabe and Shuta Takayanagi and Motohide Aoki and Tomonari Umemura and Julian J Eaton-Rye and Frances D Pitt and Mikio Tsuzuki},
\r\nurl = {http:\/\/www.sciencedirect.com\/science\/article\/pii\/S2211926418307380},
\r\ndoi = {https:\/\/doi.org\/10.1016\/j.algal.2019.101410},
\r\nissn = {2211-9264},
\r\nyear  = {2019},
\r\ndate = {2019-01-01},
\r\njournal = {Algal Research},
\r\nvolume = {38},
\r\npages = {101410},
\r\nabstract = {Phosphate transporters, which take up not only phosphate but also arsenate, have attracted attention for phosphorus (P) resource recovery from eutrophic environments and arsenic (As) removal from polluted areas by bioremediation. The cyanobacterium Synechocystis sp. PCC 6803 can grow in a higher ratio of arsenate (150\u202fmM) to phosphate (0.2\u202fmM) in comparison with plants and microalgae. Analysis of the As resistance of the Pst1 and Pst2 phosphate transporter deletion mutants (\u0394pst1 and \u0394pst2) showed that the \u0394pst1 strain is more sensitive to arsenate under P-replete conditions but more resistant under P-deplete conditions compared with \u0394pst2 cells and wild type. This pattern in sensitivity is attributed to the levels of initial As accumulation and pst gene expression. Moreover, the ratio of phosphate uptake to arsenate uptake rates at 10\u202f\u03bcM was 7\u201310:1 in the wild type and the \u0394pst2 mutant, while that in \u0394pst1 and \u0394 pstS1 (phosphate-binding protein from the pst1 gene cluster), were about 1.5:1, suggesting a high selectivity of Pst1 for phosphate.},
\r\nkeywords = {},
\r\npubstate = {published},
\r\ntppubtype = {article}
\r\n}
\r\n<\/pre><\/div>
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\r\n

2018<\/h3>\r\n <\/td>\r\n <\/tr>

Fujimori, Eiji; Nagata, Suzuka; Kumata, Hidetoshi; Umemura, Tomonari<\/p>

Investigation of adverse effect of coexisting aminopolycarboxylates on the determination of rare earth elements by ICP-MS after solid phase extraction using an iminodiacetate-based chelating-resin<\/a> Journal Article<\/span> <\/p>

In: <\/span>Chemosphere, <\/span>vol. 214, <\/span>pp. 288-294, <\/span>2018<\/span>.<\/p>

Abstract<\/a><\/span> | Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

@article{Fujimori2018,
\r\ntitle = {Investigation of adverse effect of coexisting aminopolycarboxylates on the determination of rare earth elements by ICP-MS after solid phase extraction using an iminodiacetate-based chelating-resin},
\r\nauthor = {Eiji Fujimori and Suzuka Nagata and Hidetoshi Kumata and Tomonari Umemura},
\r\ndoi = {10.1016\/j.chemosphere.2018.09.073},
\r\nyear  = {2018},
\r\ndate = {2018-09-14},
\r\njournal = {Chemosphere},
\r\nvolume = {214},
\r\npages = {288-294},
\r\nabstract = {When iminodiacetic acid chelating-resin solid phase extraction (SPE) was used for the preconcentration of rare earth elements (REEs) in river water samples around sewage treatment plant (STP), low recovery values for heavy REEs were observed. In order to find out the reason for the low recovery, in the present paper, organic ligands in the STP effluent, which may compete with iminodiacetic acids, were analyzed by GC-NPD. It was found that EDTA was contained in the STP effluent at several-100\u202fnM level and interfered with the adsorption of REEs, especially heavy REEs (present at pM level) on the chelating-resin due to the formation of stable complexes. Therefore, acid treatment was applied to decompose EDTA molecules. As a result of acid treatment with HNO3 and H2O2 at 170\u202f\u00b0C for 4\u202fh, all REEs were almost quantitatively recovered from the STP effluent with chelating-resin SPE with good reproducibility. After the acid treatment and subsequent 40-times preconcentration with SPE, all REEs in river water samples were precisely determined by ICP-MS at several-10 to sub pg mL\u22121 levels.},
\r\nkeywords = {},
\r\npubstate = {published},
\r\ntppubtype = {article}
\r\n}
\r\n<\/pre><\/div>
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Sakurada, Shunto; Fujiwara, Shoko; Suzuki, Michio; Kogure, Toshihiro; Uchida, Tatsuya; Umemura, Tomonari; Tsuzuki, Mikio<\/p>

Involvement of Acidic Polysaccharide Ph-PS-2 and Protein in Initiation of Coccolith Mineralization, as Demonstrated by In Vitro Calcification on the Base Plate<\/a> Journal Article<\/span> <\/p>

In: <\/span>Marine Biotechnology, <\/span>vol. 20, <\/span>no. 3, <\/span>pp. 304\u2013312, <\/span>2018<\/span>, ISSN: 1436-2236<\/span>.<\/p>

Abstract<\/a><\/span> | Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

@article{Sakurada2018,
\r\ntitle = {Involvement of Acidic Polysaccharide Ph-PS-2 and Protein in Initiation of Coccolith Mineralization, as Demonstrated by In Vitro Calcification on the Base Plate},
\r\nauthor = {Shunto Sakurada and Shoko Fujiwara and Michio Suzuki and Toshihiro Kogure and Tatsuya Uchida and Tomonari Umemura and Mikio Tsuzuki},
\r\nurl = {https:\/\/doi.org\/10.1007\/s10126-018-9818-4},
\r\ndoi = {10.1007\/s10126-018-9818-4},
\r\nissn = {1436-2236},
\r\nyear  = {2018},
\r\ndate = {2018-06-01},
\r\njournal = {Marine Biotechnology},
\r\nvolume = {20},
\r\nnumber = {3},
\r\npages = {304--312},
\r\nabstract = {Coccolithophorids, unicellular marine microalgae, have calcified scales with elaborate structures, called coccoliths, on the cell surface. Coccoliths generally comprise a base plate, CaCO3, and a crystal coat consisting of acidic polysaccharides. In this study, the in vitro calcification conditions on the base plate of Pleurochrysis haptonemofera were examined to determine the functions of the base plate and acidic polysaccharides (Ph-PS-1, -2, and -3). When EDTA-treated coccoliths (acidic polysaccharide-free base plates) or low pH-treated coccoliths (whole acidic polysaccharide-containing base plates) were used, mineralization was not detected on the base plate. In contrast, in the case of coccoliths which were decalcified by lowering of the pH and then treated with urea (Ph-PS-2-containing base plates), distinct aggregates, probably containing CaCO3, were observed only on the rim of the base plates. Energy dispersive X-ray spectroscopy (EDS) confirmed that the aggregates contained Ca and O, although X-ray diffraction analysis did not reveal any evidence of crystalline materials. Also, in vitro mineralization experiments performed on EDTA-treated coccoliths using isolated acidic polysaccharides demonstrated that the Ca-containing aggregates were markedly formed only in the presence of Ph-PS-2. Furthermore, in vitro mineralization experiments conducted on protein-extracted base plates suggested that the coccolith-associated protein(s) are involved in the Ca deposition. These findings suggest that Ph-PS-2 associated with the protein(s) on the base plate rim initiates Ca2+ binding at the beginning of coccolith formation, and some other factors are required for subsequent calcite formation.},
\r\nkeywords = {},
\r\npubstate = {published},
\r\ntppubtype = {article}
\r\n}
\r\n<\/pre><\/div>
Close<\/a><\/p><\/div>
Coccolithophorids, unicellular marine microalgae, have calcified scales with elaborate structures, called coccoliths, on the cell surface. Coccoliths generally comprise a base plate, CaCO3, and a crystal coat consisting of acidic polysaccharides. In this study, the in vitro calcification conditions on the base plate of Pleurochrysis haptonemofera were examined to determine the functions of the base plate and acidic polysaccharides (Ph-PS-1, -2, and -3). When EDTA-treated coccoliths (acidic polysaccharide-free base plates) or low pH-treated coccoliths (whole acidic polysaccharide-containing base plates) were used, mineralization was not detected on the base plate. In contrast, in the case of coccoliths which were decalcified by lowering of the pH and then treated with urea (Ph-PS-2-containing base plates), distinct aggregates, probably containing CaCO3, were observed only on the rim of the base plates. Energy dispersive X-ray spectroscopy (EDS) confirmed that the aggregates contained Ca and O, although X-ray diffraction analysis did not reveal any evidence of crystalline materials. Also, in vitro mineralization experiments performed on EDTA-treated coccoliths using isolated acidic polysaccharides demonstrated that the Ca-containing aggregates were markedly formed only in the presence of Ph-PS-2. Furthermore, in vitro mineralization experiments conducted on protein-extracted base plates suggested that the coccolith-associated protein(s) are involved in the Ca deposition. These findings suggest that Ph-PS-2 associated with the protein(s) on the base plate rim initiates Ca2+ binding at the beginning of coccolith formation, and some other factors are required for subsequent calcite formation.<\/div>
Close<\/a><\/p><\/div>

Yagi, Sota; Akanuma, Satoshi; Kaji, Asumi; Niiro, Hiroya; Akiyama, Hayato; Uchida, Tatsuya; Yamagishi, Akihiko.<\/p>

Selection of a platinum-binding sequence in a loop of a four-helix bundle protein.<\/a> Journal Article<\/span> <\/p>

In: <\/span>Journal of Bioscience and Bioengineering, <\/span>vol. 125, <\/span>no. 2, <\/span>pp. 192\u2013198, <\/span>2018<\/span>, ISSN: 1347-4421<\/span>.<\/p>

Abstract<\/a><\/span> | Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

@article{Yagi2018,
\r\ntitle = {Selection of a platinum-binding sequence in a loop of a four-helix bundle protein.},
\r\nauthor = {Sota Yagi and Satoshi Akanuma and Asumi Kaji and Hiroya Niiro and Hayato Akiyama and Tatsuya Uchida and Akihiko. Yamagishi},
\r\ndoi = {10.1016\/j.jbiosc.2017.09.006},
\r\nissn = {1347-4421},
\r\nyear  = {2018},
\r\ndate = {2018-01-01},
\r\njournal = {Journal of Bioscience and Bioengineering},
\r\nvolume = {125},
\r\nnumber = {2},
\r\npages = {192--198},
\r\npublisher = {Society for Biotechnology, Japan},
\r\nabstract = {Protein-metal hybrids are functional materials with various industrial applications. For example, a redox enzyme immobilized on a platinum electrode is a key component of some biofuel cells and biosensors. To create these hybrid materials, protein mols. are bound to metal surfaces. Here, we report the selection of a novel platinum-binding sequence in a loop of a four-helix bundle protein, the Lac repressor four-helix protein (LARFH), an artificial protein in which four identical $alpha$-helixes are connected via three identical loops. We created a genetic library in which the Ser-Gly-Gln-Gly-Gly-Ser sequence within the first inter-helical loop of LARFH was semi-randomly mutated. The library was then subjected to selection for platinum-binding affinity by using the T7 phage display method. The majority of the selected variants contained the Tyr-Lys-Arg-Gly-Tyr-Lys (YKRGYK) sequence in their randomized segment. We characterized the platinum-binding properties of mutant LARFH by using quartz crystal microbalance anal. Mutant LARFH seemed to interact with platinum through its loop contg. the YKRGYK sequence, as judged by the estd. exclusive area occupied by a single mol. Furthermore, a 10-residue peptide contg. the YKRGYK sequence bound to platinum with reasonably high affinity and basic side chains in the peptide were crucial in mediating this interaction. In conclusion, we have identified an amino acid sequence, YKRGYK, in the loop of a helix-loop-helix motif that shows high platinum-binding affinity. This sequence could be grafted into loops of other polypeptides as an approach to immobilize proteins on platinum electrodes for use as biosensors among other applications. [on SciFinder(R)]},
\r\nkeywords = {},
\r\npubstate = {published},
\r\ntppubtype = {article}
\r\n}
\r\n<\/pre><\/div>
Close<\/a><\/p><\/div>
Protein-metal hybrids are functional materials with various industrial applications. For example, a redox enzyme immobilized on a platinum electrode is a key component of some biofuel cells and biosensors. To create these hybrid materials, protein mols. are bound to metal surfaces. Here, we report the selection of a novel platinum-binding sequence in a loop of a four-helix bundle protein, the Lac repressor four-helix protein (LARFH), an artificial protein in which four identical $alpha$-helixes are connected via three identical loops. We created a genetic library in which the Ser-Gly-Gln-Gly-Gly-Ser sequence within the first inter-helical loop of LARFH was semi-randomly mutated. The library was then subjected to selection for platinum-binding affinity by using the T7 phage display method. The majority of the selected variants contained the Tyr-Lys-Arg-Gly-Tyr-Lys (YKRGYK) sequence in their randomized segment. We characterized the platinum-binding properties of mutant LARFH by using quartz crystal microbalance anal. Mutant LARFH seemed to interact with platinum through its loop contg. the YKRGYK sequence, as judged by the estd. exclusive area occupied by a single mol. Furthermore, a 10-residue peptide contg. the YKRGYK sequence bound to platinum with reasonably high affinity and basic side chains in the peptide were crucial in mediating this interaction. In conclusion, we have identified an amino acid sequence, YKRGYK, in the loop of a helix-loop-helix motif that shows high platinum-binding affinity. This sequence could be grafted into loops of other polypeptides as an approach to immobilize proteins on platinum electrodes for use as biosensors among other applications. [on SciFinder(R)]<\/div>
Close<\/a><\/p><\/div>

Aoki, Motohide; Sato, Norihiro<\/p>

Fatty Acid Content and Composition of Triacylglycerols of Chlorella kessleri<\/a> Journal Article<\/span> <\/p>

In: <\/span>Bio-Protocol, <\/span>vol. 7, <\/span>no. 1, <\/span>pp. 1\u20139, <\/span>2018<\/span>, ISSN: 2331-8325<\/span>.<\/p>

Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

Hirose, Atsumi; Kasai, Takuya; Aoki, Motohide; Umemura, Tomonari; Watanabe, Kazuya; Kouzuma, Atsushi<\/p>

Electrochemically active bacteria sense electrode potentials for regulating catabolic pathways<\/a> Journal Article<\/span> <\/p>

In: <\/span>Nature communications, <\/span>vol. 9, <\/span>no. 1, <\/span>pp. 1083, <\/span>2018<\/span>, ISSN: 2041-1723<\/span>.<\/p>

Abstract<\/a><\/span> | Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

@article{Hirose2018,
\r\ntitle = {Electrochemically active bacteria sense electrode potentials for regulating catabolic pathways},
\r\nauthor = {Atsumi Hirose and Takuya Kasai and Motohide Aoki and Tomonari Umemura and Kazuya Watanabe and Atsushi Kouzuma},
\r\nurl = {https:\/\/www.nature.com\/articles\/s41467-018-03416-4},
\r\ndoi = {10.1038\/s41467-018-03416-4},
\r\nissn = {2041-1723},
\r\nyear  = {2018},
\r\ndate = {2018-01-01},
\r\njournal = {Nature communications},
\r\nvolume = {9},
\r\nnumber = {1},
\r\npages = {1083},
\r\nabstract = {Electrochemically active bacteria (EAB) receive considerable attention for their utility in bioelectrochemical processes. Although electrode potentials are known to affect the metabolic activity of EAB, it is unclear whether EAB are able to sense and respond to electrode potentials. Here, we show that, in the presence of a high-potential electrode, a model EAB Shewanella oneidensis MR-1 can utilize NADH-dependent catabolic pathways and a background formate-dependent pathway to achieve high growth yield. We also show that an Arc regulatory system is involved in sensing electrode potentials and regulating the expression of catabolic genes, including those for NADH dehydrogenase. We suggest that these findings may facilitate the use of EAB in biotechnological processes and offer the molecular bases for their ecological strategies in natural habitats.},
\r\nkeywords = {},
\r\npubstate = {published},
\r\ntppubtype = {article}
\r\n}
\r\n<\/pre><\/div>
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\r\n

2017<\/h3>\r\n <\/td>\r\n <\/tr>

Tani, H; Okuda, S; Nakamura, K; Aoki, M; Umemura, T<\/p>

Short-lived long non-coding RNAs as surrogate indicators for chemical exposure and LINC00152 and MALAT1 modulate their neighboring genes<\/a> Journal Article<\/span> <\/p>

In: <\/span>PloS one, <\/span>vol. 12, <\/span>no. 7, <\/span>2017<\/span>, ISSN: 19326203<\/span>.<\/p>

Abstract<\/a><\/span> | Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

@article{Tani2017,
\r\ntitle = {Short-lived long non-coding RNAs as surrogate indicators for chemical exposure and LINC00152 and MALAT1 modulate their neighboring genes},
\r\nauthor = {H Tani and S Okuda and K Nakamura and M Aoki and T Umemura},
\r\ndoi = {10.1371\/journal.pone.0181628},
\r\nissn = {19326203},
\r\nyear  = {2017},
\r\ndate = {2017-01-01},
\r\njournal = {PloS one},
\r\nvolume = {12},
\r\nnumber = {7},
\r\nabstract = {textcopyright 2017 Tani et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Whole transcriptome analyses have revealed a large number of novel long non-coding RNAs (lncRNAs). Although accumulating evidence demonstrates that lncRNAs play important roles in regulating gene expression, the detailed mechanisms of action of most lncRNAs remain unclear. We previously reported that a novel class of lncRNAs with a short half-life (t 1\/2 textless 4 h) in HeLa cells, termed short-lived non-coding transcripts (SLiTs), are closely associated with physiological and pathological functions. In this study, we focused on 26 SLiTs and nuclear-enriched abundant lncRNA, MALAT1(t 1\/2 of 7.6 h in HeLa cells) in neural stem cells (NSCs) derived from human induced pluripotent stem cells, and identified four SLiTs (TUG1, GAS5, FAM222-AS1, and SNHG15) that were affected by the following typical chemical stresses (oxidative stress, heavy metal stress and protein synthesis stress). We also found the expression levels of LINC00152 (t 1\/2 of 2.1 h in NSCs), MALAT1 (t 1\/2 of 1.8 h in NSCs), and their neighboring genes were elevated proportionally to the chemical doses. Moreover, we confirmed that the overexpression of LINC00152 or MALAT1 upregulated the expressions of their neighboring genes even in the absence of chemical stress. These results reveal that LINC00152 and MALAT1 modulate their neighboring genes, and thus provide a deeper understanding of the functions of lncRNAs.},
\r\nkeywords = {},
\r\npubstate = {published},
\r\ntppubtype = {article}
\r\n}
\r\n<\/pre><\/div>
Close<\/a><\/p><\/div>
textcopyright 2017 Tani et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Whole transcriptome analyses have revealed a large number of novel long non-coding RNAs (lncRNAs). Although accumulating evidence demonstrates that lncRNAs play important roles in regulating gene expression, the detailed mechanisms of action of most lncRNAs remain unclear. We previously reported that a novel class of lncRNAs with a short half-life (t 1\/2 textless 4 h) in HeLa cells, termed short-lived non-coding transcripts (SLiTs), are closely associated with physiological and pathological functions. In this study, we focused on 26 SLiTs and nuclear-enriched abundant lncRNA, MALAT1(t 1\/2 of 7.6 h in HeLa cells) in neural stem cells (NSCs) derived from human induced pluripotent stem cells, and identified four SLiTs (TUG1, GAS5, FAM222-AS1, and SNHG15) that were affected by the following typical chemical stresses (oxidative stress, heavy metal stress and protein synthesis stress). We also found the expression levels of LINC00152 (t 1\/2 of 2.1 h in NSCs), MALAT1 (t 1\/2 of 1.8 h in NSCs), and their neighboring genes were elevated proportionally to the chemical doses. Moreover, we confirmed that the overexpression of LINC00152 or MALAT1 upregulated the expressions of their neighboring genes even in the absence of chemical stress. These results reveal that LINC00152 and MALAT1 modulate their neighboring genes, and thus provide a deeper understanding of the functions of lncRNAs.<\/div>
Close<\/a><\/p><\/div>
\r\n

2016<\/h3>\r\n <\/td>\r\n <\/tr>

Sakai, Y.; Kotani, A.; Umemura, T.; Mori, Y.; Kusu, F.; Yamamoto, K.; Hakamata, H.<\/p>

Electrochemical Determination of Synephrine by Hydrophilic Interaction Liquid Chromatography Using a Zwitterionic Monolith Column<\/a> Journal Article<\/span> <\/p>

In: <\/span>Electroanalysis, <\/span>vol. 28, <\/span>pp. 1947-1951, <\/span>2016<\/span>.<\/p>

Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

Aoki, Motohide; Tsuzuki, Mikio; Sato, Norihiro<\/p>

Quantitation of Cytochromes b559, b6, and f, and the Core Component of Photosystem I P700 in Cyanobacterial Cells<\/a> Journal Article<\/span> <\/p>

In: <\/span>Bio-Protocol, <\/span>vol. 6, <\/span>no. 21, <\/span>pp. 1\u20136, <\/span>2016<\/span>.<\/p>

Abstract<\/a><\/span> | Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

@article{Aoki2016,
\r\ntitle = {Quantitation of Cytochromes b559, b6, and f, and the Core Component of Photosystem I P700 in Cyanobacterial Cells},
\r\nauthor = {Motohide Aoki and Mikio Tsuzuki and Norihiro Sato},
\r\ndoi = {10.21769\/BioProtoc.1991},
\r\nyear  = {2016},
\r\ndate = {2016-01-01},
\r\njournal = {Bio-Protocol},
\r\nvolume = {6},
\r\nnumber = {21},
\r\npages = {1--6},
\r\nabstract = {Cytochrome (Cyt) b559, an important and essential core component of photosystem II in the photosynthetic electron transport system, is a heme-bridged heterodimer protein composed of an alpha subunit (PsbE) and a beta subunit (PsbF), and its reduced form has an absorption maximum in the $alpha$- band at 559 nm. The amounts of Cyt b559 can be determined by spectrophotometrical measurement of reduced minus oxidized difference spectra that are normalized with absorbance of isosbestic point at 580 nm. The authors use differential extinction coefficients of Cyt b559 [$Delta$$epsilon$(559-580 nm) = 15.5 mM-1textperiodcenteredcm-1], which have been reported by Garewal and Wasserman (1974). In addition to the Cyt b559, this procedure can be used for quantitation of Cyt b6 and Cyt f, the subunits of the Cyt b6\/f complex, and P700, one of the core components of photosystem I. This protocol, which is adapted from Fujita and Murakami (1987), is used in a cyanobacterium, Synechococcus elongatus PCC 7942, and also in other cyanobacterial strains including Synechocystis sp. PCC 6803.},
\r\nkeywords = {},
\r\npubstate = {published},
\r\ntppubtype = {article}
\r\n}
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Chikamatsu, Kazuhiro; Aoki, Motohide; Uchida, Tatsuya; Umemura, Tomonari; Kumata, Hidetoshi<\/p>

Optimization of the Extraction Solvent for Polycyclic Aromatic Hydrocarbons (PAHs) from Road Dusts by Using Pressurized Solvent Extraction and Evaluation of Its Impact on Source Diagnostic Isomer Pair Ratios of PAHs<\/a> Journal Article<\/span> <\/p>

In: <\/span>Bunseki Kagaku, <\/span>vol. 65, <\/span>no. 4, <\/span>pp. 193\u2013202, <\/span>2016<\/span>.<\/p>

Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

Sakurai, Toshihiro; Aoki, Motohide; Ju, Xiaohui; Ueda, Tatsuya; Nakamura, Yasunori; Fujiwara, Shoko; Umemura, Tomonari; Tsuzuki, Mikio; Minoda, Ayumi<\/p>

Profiling of lipid and glycogen accumulations under different growth conditions in the sulfothermophilic red alga Galdieria sulphuraria<\/a> Journal Article<\/span> <\/p>

In: <\/span>Bioresource Technology, <\/span>vol. 200, <\/span>pp. 861\u2013866, <\/span>2016<\/span>, ISSN: 18732976<\/span>.<\/p>

Abstract<\/a><\/span> | Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

@article{Sakurai2016,
\r\ntitle = {Profiling of lipid and glycogen accumulations under different growth conditions in the sulfothermophilic red alga Galdieria sulphuraria},
\r\nauthor = {Toshihiro Sakurai and Motohide Aoki and Xiaohui Ju and Tatsuya Ueda and Yasunori Nakamura and Shoko Fujiwara and Tomonari Umemura and Mikio Tsuzuki and Ayumi Minoda},
\r\nurl = {http:\/\/dx.doi.org\/10.1016\/j.biortech.2015.11.014},
\r\ndoi = {10.1016\/j.biortech.2015.11.014},
\r\nissn = {18732976},
\r\nyear  = {2016},
\r\ndate = {2016-01-01},
\r\njournal = {Bioresource Technology},
\r\nvolume = {200},
\r\npages = {861--866},
\r\npublisher = {Elsevier Ltd},
\r\nabstract = {The unicellular red alga Galdieria sulphuraria grows efficiently and produces a large amount of biomass in acidic conditions at high temperatures. It has great potential to produce biofuels and other beneficial compounds without becoming contaminated with other organisms. In G. sulphuraria, biomass measurements and glycogen and lipid analyses demonstrated that the amounts and compositions of glycogen and lipids differed when cells were grown under autotrophic, mixotrophic, and heterotrophic conditions. Maximum biomass production was obtained in the mixotrophic culture. High amounts of glycogen were obtained in the mixotrophic cultures, while the amounts of neutral lipids were similar between mixotrophic and heterotrophic cultures. The amounts of neutral lipids were highest in red algae, including thermophiles. Glycogen structure and fatty acids compositions largely depended on the growth conditions.},
\r\nkeywords = {},
\r\npubstate = {published},
\r\ntppubtype = {article}
\r\n}
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Sato, Norihiro; Kobayashi, Satomi; Aoki, Motohide; Umemura, Tomonari; Kobayashi, Isao; Tsuzuki, Mikio<\/p>

Identification of genes for sulfolipid synthesis in primitive red alga Cyanidioschyzon merolae<\/a> Journal Article<\/span> <\/p>

In: <\/span>Biochemical and Biophysical Research Communications, <\/span>vol. 470, <\/span>no. 1, <\/span>pp. 123\u2013129, <\/span>2016<\/span>, ISSN: 0006291X<\/span>.<\/p>

Abstract<\/a><\/span> | Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

@article{Sato2016,
\r\ntitle = {Identification of genes for sulfolipid synthesis in primitive red alga Cyanidioschyzon merolae},
\r\nauthor = {Norihiro Sato and Satomi Kobayashi and Motohide Aoki and Tomonari Umemura and Isao Kobayashi and Mikio Tsuzuki},
\r\nurl = {http:\/\/www.sciencedirect.com\/science\/article\/pii\/S0006291X16300067},
\r\ndoi = {10.1016\/j.bbrc.2016.01.006},
\r\nissn = {0006291X},
\r\nyear  = {2016},
\r\ndate = {2016-01-01},
\r\njournal = {Biochemical and Biophysical Research Communications},
\r\nvolume = {470},
\r\nnumber = {1},
\r\npages = {123--129},
\r\npublisher = {Elsevier Ltd},
\r\nabstract = {Sulfoquinovosyl diacylglycerol is one of the lipids that construct thylakoid membranes, and is distributed from cyanobacteria to plastids in plants including a red lineage. One of the most primitive red algae, Cyanidioschyzon melorae, similar to cyanobacteria and green plants, possesses homologs of the SQD1 and SQD2 genes that code for UDP-sulfoquinovose and sulfoquinovosyl diacylglycerol synthases, respectively, for the synthesis of sulfoquinovosyl diacylglycerol. We here revealed the structural properties of SQD1 and SQD2 homologs in C. melorae intrinsic to those of the authentic proteins, and verified their enzymatic functions through heterologous expression in cyanobacterial disruptants as to the corresponding genes. The results demonstrated that the system of sulfoqunovosyl diacylglycerol synthesis could have been conserved through evolution of cyanobacteria to plastids in a red lineage, which is compatible with the monophyletic origin of plastids.},
\r\nkeywords = {},
\r\npubstate = {published},
\r\ntppubtype = {article}
\r\n}
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2015<\/h3>\r\n <\/td>\r\n <\/tr>

Honda, Mao; Kitajima, Nobuyuki; Abe, Tomoko; Umemura, Tomonari; Hokura, Akiko.<\/p>

Study on chromium accumulation mechanism of Chinese break fern (Pteris vittata L.) by synchrotron radiation X-ray fluorescence analysis.<\/a> Journal Article<\/span> <\/p>

In: <\/span>Bunseki Kagaku, <\/span>vol. 64, <\/span>no. 11, <\/span>pp. 801\u2013810, <\/span>2015<\/span>, ISSN: 0525-1931<\/span>.<\/p>

Abstract<\/a><\/span> | Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

@article{Honda2015,
\r\ntitle = {Study on chromium accumulation mechanism of Chinese break fern (Pteris vittata L.) by synchrotron radiation X-ray fluorescence analysis.},
\r\nauthor = {Honda, Mao and Kitajima, Nobuyuki and Abe, Tomoko and Umemura, Tomonari and Hokura, Akiko.},
\r\ndoi = {10.2116\/bunsekikagaku.64.801},
\r\nissn = {0525-1931},
\r\nyear  = {2015},
\r\ndate = {2015-01-01},
\r\njournal = {Bunseki Kagaku},
\r\nvolume = {64},
\r\nnumber = {11},
\r\npages = {801--810},
\r\npublisher = {Nippon Bunseki Kagakkai},
\r\nabstract = {Pteris vittata L. is known as an arsenic hyper-accumulator. To understand the tolerance mechanism, this study investigated the speciation and distribution of chromium accumulated in fern using synchrotron-radiation X-ray fluorescence anal. The plants were sep. treated with K2Cr2O7 and CrCl3\u00b7H2O in a hydroponic system. The chem. speciations of chromium and sulfur in the fern were carried out by X-ray absorption near edge structure (XANES) anal.; the elemental distributions in their tissues were visualized by micro-XRF imaging. Chromium was highly accumulated in the roots. $mu$-XANES and XANES indicated that highly toxic Cr(VI) was reduced Cr(III) as less-toxic Cr(III). The fern-cultivated Cr(III) was accumulated as Cr(III). The chem. speciation of sulfur showed that the ratio of S(-II) compds., such as glutathione (GSH) and cysteine in the roots, increased when was treated with Cr(VI) and Cr(III). This result demonstrated that sulfur had a certain function in the detoxification process of P. vittata L. [on SciFinder(R)]},
\r\nkeywords = {},
\r\npubstate = {published},
\r\ntppubtype = {article}
\r\n}
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Suzuki, Yusuke; Takenaka, Chisato; Tomioka, Rie; Tsubota, Hiromi; Takasaki, Yuka; Umemura, Tomonari.<\/p>

Accumulation of Arsenic and Copper by Bryophytes Growing in an Aquatic Environment near Copper Mine Tailings.<\/a> Journal Article<\/span> <\/p>

In: <\/span>Mine Water and the Environment, <\/span>pp. Ahead of Print, <\/span>2015<\/span>, ISSN: 1025-9112<\/span>.<\/p>

Abstract<\/a><\/span> | Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

@article{Suzuki2015,
\r\ntitle = {Accumulation of Arsenic and Copper by Bryophytes Growing in an Aquatic Environment near Copper Mine Tailings.},
\r\nauthor = {Suzuki, Yusuke and Takenaka, Chisato and Tomioka, Rie and Tsubota, Hiromi and Takasaki, Yuka and Umemura, Tomonari.},
\r\ndoi = {10.1007\/s10230-015-0335-7},
\r\nissn = {1025-9112},
\r\nyear  = {2015},
\r\ndate = {2015-01-01},
\r\njournal = {Mine Water and the Environment},
\r\npages = {Ahead of Print},
\r\npublisher = {Springer GmbH},
\r\nabstract = {Bryophytes with high As accumulation affinity were identified in the aquatic environment. We surveyed a stream near copper mine tailings and then conducted lab. expts. to confirm As accumulation in the bryophytes with high As affinity. We found that a moss, Scopelophila cataractae, accumulates As in addn. to Cu in aquatic environments and confirmed it in lab. expts. The highest value for As in S. cataractae from the field survey was 1300 mg\/kg dry wt. at relatively low As concns. in the stream water (0.005 mg\/L). In addn., Brachythecium plumosum and Rhynchostegium riparioides may also be useful bryophytes for accumulation of Cu and As, though the mechanisms of As accumulation might differ between these two bryophytes and S. cataractae. [on SciFinder(R)]},
\r\nkeywords = {},
\r\npubstate = {published},
\r\ntppubtype = {article}
\r\n}
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\r\n

2014<\/h3>\r\n <\/td>\r\n <\/tr>

Yanai, Hikaru; Egawa, Saki; Yamada, Kenta; Ono, Junpei; Aoki, Motohide; Matsumoto, Takashi; Taguchi, Takeo<\/p>

1,1-Bis(triflyl)alkadienes: Easy-To-Handle Building Blocks for Strongly Acidic Carbon Acids<\/a> Journal Article<\/span> <\/p>

In: <\/span>Asian Journal of Organic Chemistry, <\/span>vol. 3, <\/span>no. 4, <\/span>pp. 556\u2013563, <\/span>2014<\/span>, ISSN: 21935807<\/span>.<\/p>

Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

Aoki, Motohide; Nakamura, Yoshisuke; Akamine, Runa; Kumata, Hidetoshi; Umemura, Tomonari<\/p>

Discovery of the cyanobacterial biomarker respond to toxic metal based on the lipidomics Journal Article<\/span> <\/p>

In: <\/span>Biomedical Research on Trace Elements, <\/span>vol. 25, <\/span>no. 2, <\/span>pp. 83, <\/span>2014<\/span>, ISBN: 0916-717X<\/span>.<\/p>

BibTeX<\/a><\/span><\/p>

\r\n

2013<\/h3>\r\n <\/td>\r\n <\/tr>

Inoue, Kana; Aoki, Motohide; Fujiwara, Kitao<\/p>

Protein cohesion induced by metal ions observed with fluorescence correlation spectroscopy<\/a> Journal Article<\/span> <\/p>

In: <\/span>Journal of Environmental Science and Health, Part A, <\/span>vol. 48, <\/span>no. 11, <\/span>pp. 1311\u20131317, <\/span>2013<\/span>, ISSN: 1093-4529<\/span>.<\/p>

Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

Groombridge, Alexander S; Miyashita, Shin-ichi; Fujii, Shin-ichiro; Nagasawa, Keisuke; Okahashi, Tetsuya; Ohata, Masaki; Umemura, Tomonari; Takatsu, Akiko; Inagaki, Kazumi; Chiba, Koichi.<\/p>

High sensitive elemental analysis of single yeast cells (Saccharomyces cerevisiae) by time-resolved inductively-coupled plasma mass spectrometry using a high efficiency cell introduction system.<\/a> Journal Article<\/span> <\/p>

In: <\/span>Analytical Sciences, <\/span>vol. 29, <\/span>no. 6, <\/span>pp. 597\u2013603, <\/span>2013<\/span>, ISSN: 0910-6340<\/span>.<\/p>

Abstract<\/a><\/span> | Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

@article{Groombridge2013,
\r\ntitle = {High sensitive elemental analysis of single yeast cells (Saccharomyces cerevisiae) by time-resolved inductively-coupled plasma mass spectrometry using a high efficiency cell introduction system.},
\r\nauthor = {Groombridge, Alexander S and Miyashita, Shin-ichi and Fujii, Shin-ichiro and Nagasawa, Keisuke and Okahashi, Tetsuya and Ohata, Masaki and Umemura, Tomonari and Takatsu, Akiko and Inagaki, Kazumi and Chiba, Koichi.},
\r\ndoi = {10.2116\/analsci.29.597},
\r\nissn = {0910-6340},
\r\nyear  = {2013},
\r\ndate = {2013-01-01},
\r\njournal = {Analytical Sciences},
\r\nvolume = {29},
\r\nnumber = {6},
\r\npages = {597--603},
\r\npublisher = {Japan Society for Analytical Chemistry},
\r\nabstract = {Trace elemental anal. of single yeast cells with time-resolved inductively coupled plasma mass spectrometry (ICP-MS) was successfully carried out, where a high efficiency cell introduction system (HECIS) consisting of the high performance concentric nebulizer (HPCN) and a low-vol. (15 mL) on-axis spray chamber utilizing a sheath gas flow were used. Cell adsorption to the flow injector and sample tubing was reduced with the addn. of a simple 4.3 mmol L-1 of NaCl soln. to the cell suspension and cell flowing liq., allowing consecutive measurements without fear of significant contamination from previous measurements. Initially using a quadrupole mass analyzer ICP-MS (ICP-QMS) at its lowest integration time (10 ms), current spikes corresponding to sep. cell events were detected for several elements (Mg, P, Ca, Mn, Fe, Cu, and Zn) on the introduction of the cell suspension. On comparing the no. of peaks in the spectrum for phosphorous with the cell count using a haemocytometer, a reproducible cell transport efficiency of 75.0 \u00b1 4.7% was achieved. Preliminary expts. into using time of flight ICP-MS (ICP-TOFMS) for single-cell anal. were carried out, allowing quasi-simultaneous multielement detection. The spectra of Mg, P, Ca, Mn, Fe, Cu, and Zn, with a time resoln. of 1 ms were simultaneously obtained in one measurement. A relatively strong correlation was obsd. for the spectra between P and Zn (correlation factor 0.69), P and Mg (0.63), and Mg and Zn (0.63). These results indicate that the time resolved quasi-simultaneous multielement measurement may be useful for the correlation anal. of multielements in cells. [on SciFinder(R)]},
\r\nkeywords = {},
\r\npubstate = {published},
\r\ntppubtype = {article}
\r\n}
\r\n<\/pre><\/div>
Close<\/a><\/p><\/div>
Trace elemental anal. of single yeast cells with time-resolved inductively coupled plasma mass spectrometry (ICP-MS) was successfully carried out, where a high efficiency cell introduction system (HECIS) consisting of the high performance concentric nebulizer (HPCN) and a low-vol. (15 mL) on-axis spray chamber utilizing a sheath gas flow were used. Cell adsorption to the flow injector and sample tubing was reduced with the addn. of a simple 4.3 mmol L-1 of NaCl soln. to the cell suspension and cell flowing liq., allowing consecutive measurements without fear of significant contamination from previous measurements. Initially using a quadrupole mass analyzer ICP-MS (ICP-QMS) at its lowest integration time (10 ms), current spikes corresponding to sep. cell events were detected for several elements (Mg, P, Ca, Mn, Fe, Cu, and Zn) on the introduction of the cell suspension. On comparing the no. of peaks in the spectrum for phosphorous with the cell count using a haemocytometer, a reproducible cell transport efficiency of 75.0 \u00b1 4.7% was achieved. Preliminary expts. into using time of flight ICP-MS (ICP-TOFMS) for single-cell anal. were carried out, allowing quasi-simultaneous multielement detection. The spectra of Mg, P, Ca, Mn, Fe, Cu, and Zn, with a time resoln. of 1 ms were simultaneously obtained in one measurement. A relatively strong correlation was obsd. for the spectra between P and Zn (correlation factor 0.69), P and Mg (0.63), and Mg and Zn (0.63). These results indicate that the time resolved quasi-simultaneous multielement measurement may be useful for the correlation anal. of multielements in cells. [on SciFinder(R)]<\/div>
Close<\/a><\/p><\/div>

Yamamoto, Kazuhiro; Komiyama, Ryo; Umemura, Tomonari.<\/p>

Numerical simulation on flow in column chromatography.<\/a> Journal Article<\/span> <\/p>

In: <\/span>International Journal of Modern Physics C: Computational Physics, Physical Computation, <\/span>vol. 24, <\/span>no. 12, <\/span>pp. 1340003\/1\u20131340003\/7, <\/span>2013<\/span>, ISSN: 0129-1831<\/span>.<\/p>

Abstract<\/a><\/span> | Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

@article{Yamamoto2013,
\r\ntitle = {Numerical simulation on flow in column chromatography.},
\r\nauthor = {Yamamoto, Kazuhiro and Komiyama, Ryo and Umemura, Tomonari.},
\r\ndoi = {10.1142\/S0129183113400032},
\r\nissn = {0129-1831},
\r\nyear  = {2013},
\r\ndate = {2013-01-01},
\r\njournal = {International Journal of Modern Physics C: Computational Physics, Physical Computation},
\r\nvolume = {24},
\r\nnumber = {12},
\r\npages = {1340003\/1--1340003\/7},
\r\npublisher = {World Scientific Publishing Co. Pte. Ltd.},
\r\nabstract = {Monolithic columns have attracted much attention as a novel platform for high throughput anal., but there is little information about the fluid profile in the flow channels. In this paper, we presented our approach for the fluid simulation in column chromatog. by the lattice Boltzmann method (LBM). To simulate the monolithic column system, the calcn. domain was modeled by the 3D channel flow through sphere obstacles. Several types of porous structure were used, with uniform and nonuniform pores. Based on the simulations results, we discussed fluid flow and pressure variation for the optimization of the suitable structure for HPLC system. [on SciFinder(R)]},
\r\nkeywords = {},
\r\npubstate = {published},
\r\ntppubtype = {article}
\r\n}
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Aoshima, Hiroshi; Satoh, Kotaro; Umemura, Tomonari; Kamigaito, Masami.<\/p>

A simple combination of higher-oxidation-state FeX3 and phosphine or amine ligand for living radical polymerization of styrene, methacrylate, and acrylate.<\/a> Journal Article<\/span> <\/p>

In: <\/span>Polymer Chemistry, <\/span>vol. 4, <\/span>no. 12, <\/span>pp. 3554\u20133562, <\/span>2013<\/span>, ISSN: 1759-9962<\/span>.<\/p>

Abstract<\/a><\/span> | Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

@article{Aoshima2013,
\r\ntitle = {A simple combination of higher-oxidation-state FeX3 and phosphine or amine ligand for living radical polymerization of styrene, methacrylate, and acrylate.},
\r\nauthor = {Aoshima, Hiroshi and Satoh, Kotaro and Umemura, Tomonari and Kamigaito, Masami.},
\r\ndoi = {10.1039\/c3py00352c},
\r\nissn = {1759-9962},
\r\nyear  = {2013},
\r\ndate = {2013-01-01},
\r\njournal = {Polymer Chemistry},
\r\nvolume = {4},
\r\nnumber = {12},
\r\npages = {3554--3562},
\r\npublisher = {Royal Society of Chemistry},
\r\nabstract = {Higher-oxidn.-state Fe halides [FeX3 (X = Cl, Br)] were employed in conjunction with ligands, mainly monodentate phosphines and amines, to effect the living radical polymn. of various vinyl monomers such as styrene, Me methacrylate (MMA), and Me acrylate (MA). Almost all combinations examd. could enable polymns. in the absence of exogenous reducing agents. However, appropriate combinations of FeX3 and ligands gave rise to polymers in a living manner, with controlled mol. wts. and narrow mol. wt. distributions (Mw\/Mn = 1.1-1.2). Ligand combinations included FeCl3 with PnBu3, P(CMe3)3, or NnBu3 (for styrene); FeCl3 with P(CMe3)3 or NnBu3 (for MMA); and FeBr3 with PPh3 (for MA). Model reactions and spectroscopic anal. suggest that FeCl3 most likely disproportionates into the Fe(III)Cl4- anion and Fe(III)Cl2+ cation in the presence of Lewis base ligands (PR3 and NR3). The latter cationic species, coordinated with the ligand [Fe(III)Cl2(PR3)+ or Fe(II)Cl2(PR3)+], acts as the active catalyst. Assistance from the electron-rich ligand allows the catalyst to induce metal-catalyzed living radical polymn. The Fe(III)-based catalyst could also be easily and almost quant. removed from the polymer product simply by washing with aq. acid to minimize the amt. of Fe contamination (<5 ppm). [on SciFinder(R)]},
\r\nkeywords = {},
\r\npubstate = {published},
\r\ntppubtype = {article}
\r\n}
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Ohta, Hiromichi; Mizoguchi, Teruyasu; Aoki, Noriyuki; Yamamoto, Takashi; Sabarudin, Akhmad; Umemura, Tomonari.<\/p>

Lithium-ion conducting La2\/3-xLi3xTiO3 solid electrolyte thin films with stepped and terraced surfaces [Retraction of document cited in CA156:547984].<\/a> Journal Article<\/span> <\/p>

In: <\/span>Applied Physics Letters, <\/span>vol. 102, <\/span>no. 8, <\/span>pp. 089902\/1, <\/span>2013<\/span>, ISSN: 0003-6951<\/span>.<\/p>

Abstract<\/a><\/span> | Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

\r\n

2012<\/h3>\r\n <\/td>\r\n <\/tr>

Takasaki, Yuka; Sakagawa, Shinnosuke; Inagaki, Kazumi; Fujii, Shin-ichiro; Sabarudin, Akhmad; Umemura, Tomonari; Haraguchi, Hiroki.<\/p>

Development of salt-tolerance interface for an high performance liquid chromatography\/inductively coupled plasma mass spectrometry system and its application to accurate quantification of DNA samples.<\/a> Journal Article<\/span> <\/p>

In: <\/span>Analytica Chimica Acta, <\/span>vol. 713, <\/span>pp. 23\u201329, <\/span>2012<\/span>, ISSN: 0003-2670<\/span>.<\/p>

Abstract<\/a><\/span> | Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

@article{Takasaki2012,
\r\ntitle = {Development of salt-tolerance interface for an high performance liquid chromatography\/inductively coupled plasma mass spectrometry system and its application to accurate quantification of DNA samples.},
\r\nauthor = {Takasaki, Yuka and Sakagawa, Shinnosuke and Inagaki, Kazumi and Fujii, Shin-ichiro and Sabarudin, Akhmad and Umemura, Tomonari and Haraguchi, Hiroki.},
\r\ndoi = {10.1016\/j.aca.2011.11.039},
\r\nissn = {0003-2670},
\r\nyear  = {2012},
\r\ndate = {2012-01-01},
\r\njournal = {Analytica Chimica Acta},
\r\nvolume = {713},
\r\npages = {23--29},
\r\npublisher = {Elsevier B.V.},
\r\nabstract = {Accurate quantification of DNA is highly important in various fields. Detn. of phosphorus by ICP-MS is one of the most effective methods for accurate quantification of DNA due to the fixed stoichiometry of phosphate to this mol. In this paper, a smart and reliable method for accurate quantification of DNA fragments and oligodeoxythymidilic acids by hyphenated HPLC\/ICP-MS equipped with a highly efficient interface device is presented. The interface was constructed of a home-made capillary-attached micronebulizer and temp.-controllable cyclonic spray chamber (IsoMist). As a sepn. column for DNA samples, home-made methacrylate-based weak anion-exchange monolith was employed. Some parameters, which include compn. of mobile phase, gradient program, inner and outer diams. of capillary, temp. of spray chamber etc., were optimized to find the best performance for sepn. and accurate quantification of DNA samples. The proposed system could achieve many advantages, such as total consumption for small amt. sample anal., salt-tolerance for hyphenated anal., high accuracy and precision for quant. anal. Using this proposed system, the samples of 20 bp DNA ladder (20, 40, 60, 80, 100, 120, 140, 160, 180, 200, 300, 400, 500 base pairs) and oligodeoxythymidilic acids (dT12-18) were rapidly sepd. and accurately quantified. [on SciFinder(R)]},
\r\nkeywords = {},
\r\npubstate = {published},
\r\ntppubtype = {article}
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Shu, Shin; Kobayashi, Hiroharu; Okubo, Masaki; Sabarudin, Akhmad; Butsugan, Michio; Umemura, Tomonari.<\/p>

Chemical anchoring of lauryl methacrylate-based reversed phase monolith to 1\/16\" o.d. polyetheretherketone tubing.<\/a> Journal Article<\/span> <\/p>

In: <\/span>Journal of Chromatography A, <\/span>vol. 1242, <\/span>pp. 59\u201366, <\/span>2012<\/span>, ISSN: 0021-9673<\/span>.<\/p>

Abstract<\/a><\/span> | Links<\/a><\/span> | BibTeX<\/a><\/span><\/p>

@article{Shu2012,
\r\ntitle = {Chemical anchoring of lauryl methacrylate-based reversed phase monolith to 1\/16\" o.d. polyetheretherketone tubing.},
\r\nauthor = {Shu, Shin and Kobayashi, Hiroharu and Okubo, Masaki and Sabarudin, Akhmad and Butsugan, Michio and Umemura, Tomonari.},
\r\ndoi = {10.1016\/j.chroma.2012.04.030},
\r\nissn = {0021-9673},
\r\nyear  = {2012},
\r\ndate = {2012-01-01},
\r\njournal = {Journal of Chromatography A},
\r\nvolume = {1242},
\r\npages = {59--66},
\r\npublisher = {Elsevier B.V.},
\r\nabstract = {The authors describe a method for the prepn. of easy-to-use reversed-phase monolithic microbore columns. Polyetheretherketone (PEEK) tubing with an outer diam. of 1\/16\" and an inner diam. of 1.0 mm was used as a column housing (empty column), and in it lauryl methacrylate (LMA) was copolymd. with ethylene dimethacrylate (EDMA). In order to chem. anchor the polymer monolith to the tube wall, the inner wall surface was pretreated by the following two-step procedure. (1) 50% sulfuric acid was filled into the PEEK tubing and left to stand for 6 h to generate sulfonate groups on the surface. (2) After washing with Milli-Q water, the sulfonated PEEK surface was brought into contact with 1 M glycidyl methacrylate in dichloromethane (or acetone) at 40\u00b0 for 4 h to introduce methacryloyl groups via the reaction of sulfonate groups and epoxy groups. Mech. strength and column efficiency of the resulting monoliths were evaluated through the sepn. of a series of alkylbenzenes in acetonitrile-water (50:50, vol.\/vol.) eluent over the flow rate range of 50-750 $mu$L\/min (corresponding to 1.7-25.5 mm\/s). The poly(LMA-co-EDMA) monolith provided acceptable column efficiency of 2000 theor. plates\/10 cm (HETP value of 50 $mu$m) for amylbenzene (sepn. factor k = 40) and low flow resistance of 0.5 MPa\/10 cm at a normal flow rate of 50 $mu$L\/min. The methacryloylated PEEK tubing tightly held the monolith, and the monolithic column exhibited good pressure resistance up to 15 MPa, allowing rapid sepn. at a 15-20 fold higher flow rate than normal. [on SciFinder(R)]},
\r\nkeywords = {},
\r\npubstate = {published},
\r\ntppubtype = {article}
\r\n}
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The authors describe a method for the prepn. of easy-to-use reversed-phase monolithic microbore columns. Polyetheretherketone (PEEK) tubing with an outer diam. of 1\/16\" and an inner diam. of 1.0 mm was used as a column housing (empty column), and in it lauryl methacrylate (LMA) was copolymd. with ethylene dimethacrylate (EDMA). In order to chem. anchor the polymer monolith to the tube wall, the inner wall surface was pretreated by the following two-step procedure. (1) 50% sulfuric acid was filled into the PEEK tubing and left to stand for 6 h to generate sulfonate groups on the surface. (2) After washing with Milli-Q water, the sulfonated PEEK surface was brought into contact with 1 M glycidyl methacrylate in dichloromethane (or acetone) at 40\u00b0 for 4 h to introduce methacryloyl groups via the reaction of sulfonate groups and epoxy groups. Mech. strength and column efficiency of the resulting monoliths were evaluated through the sepn. of a series of alkylbenzenes in acetonitrile-water (50:50, vol.\/vol.) eluent over the flow rate range of 50-750 $mu$L\/min (corresponding to 1.7-25.5 mm\/s). The poly(LMA-co-EDMA) monolith provided acceptable column efficiency of 2000 theor. plates\/10 cm (HETP value of 50 $mu$m) for amylbenzene (sepn. factor k = 40) and low flow resistance of 0.5 MPa\/10 cm at a normal flow rate of 50 $mu$L\/min. The methacryloylated PEEK tubing tightly held the monolith, and the monolithic column exhibited good pressure resistance up to 15 MPa, allowing rapid sepn. at a 15-20 fold higher flow rate than normal. [on SciFinder(R)]<\/div>
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