{"id":8,"date":"2017-11-17T23:20:47","date_gmt":"2017-11-17T14:20:47","guid":{"rendered":"http:\/\/www.ls.toyaku.ac.jp\/~bioanalchem\/en\/?page_id=8"},"modified":"2022-03-25T14:40:53","modified_gmt":"2022-03-25T05:40:53","slug":"top","status":"publish","type":"page","link":"https:\/\/www.ls.toyaku.ac.jp\/~bioanalchem\/en\/","title":{"rendered":"Top"},"content":{"rendered":"<h1 id=\"tw-target-text\" class=\"tw-data-text tw-ta tw-text-small\" dir=\"ltr\" data-placeholder=\"Translation\"><span lang=\"en\"><strong>Welcome to our laboratory&#8217;s website!!!<\/strong> <\/span><\/h1>\n<p>The mission of our laboratory is to develop new analytical methods that can measure and\/or observe directly interactions between chemical substances and living cells\/organisms, and to understand molecular, cellular, individual and ecosystem-level mechanism that life respond to the chemical environmental changes. Our long-term goal is to use the knowledge gained from our research to contribute to solution of drug design or an environmental problem.<\/p>\n<figure id=\"attachment_408\" aria-describedby=\"caption-attachment-408\" style=\"width: 720px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-408\" src=\"http:\/\/www.ls.toyaku.ac.jp\/~bioanalchem\/cms\/wp-content\/uploads\/2018\/05\/eac64e5489999cf24f72e46b984e48b2.jpg\" alt=\"\" width=\"720\" height=\"540\" \/><figcaption id=\"caption-attachment-408\" class=\"wp-caption-text\">2018 Member<\/figcaption><\/figure>\n<div id='gallery-2' class='gallery galleryid-8 gallery-columns-4 gallery-size-thumbnail'><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon portrait'>\n\t\t\t\t<a href='https:\/\/www.ls.toyaku.ac.jp\/~bioanalchem\/en\/member\/umemura-120x150\/'><img loading=\"lazy\" decoding=\"async\" width=\"120\" height=\"150\" src=\"https:\/\/www.ls.toyaku.ac.jp\/~bioanalchem\/en\/wp-content\/uploads\/sites\/2\/2018\/07\/umemura-120x150-120x150.jpg\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\" aria-describedby=\"gallery-2-61\" \/><\/a>\n\t\t\t<\/div>\n\t\t\t\t<figcaption class='wp-caption-text gallery-caption' id='gallery-2-61'>\n\t\t\t\tTomonari UMEMURA, Ph.D<\/br>\nProfessor\n\t\t\t\t<\/figcaption><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon portrait'>\n\t\t\t\t<a href='https:\/\/www.ls.toyaku.ac.jp\/~bioanalchem\/en\/member\/uchi-120x150\/'><img loading=\"lazy\" decoding=\"async\" width=\"120\" height=\"150\" src=\"https:\/\/www.ls.toyaku.ac.jp\/~bioanalchem\/en\/wp-content\/uploads\/sites\/2\/2018\/07\/uchi-120x150-120x150.jpg\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\" aria-describedby=\"gallery-2-62\" \/><\/a>\n\t\t\t<\/div>\n\t\t\t\t<figcaption class='wp-caption-text gallery-caption' id='gallery-2-62'>\n\t\t\t\tTatsuya UCHIDA, Ph.D<\/br>\nAssociate Prof.\n\t\t\t\t<\/figcaption><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon portrait'>\n\t\t\t\t<a href='https:\/\/www.ls.toyaku.ac.jp\/~bioanalchem\/en\/member\/kumata-120x150\/'><img loading=\"lazy\" decoding=\"async\" width=\"120\" height=\"150\" src=\"https:\/\/www.ls.toyaku.ac.jp\/~bioanalchem\/en\/wp-content\/uploads\/sites\/2\/2018\/07\/kumata-120x150-120x150.jpg\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\" aria-describedby=\"gallery-2-63\" \/><\/a>\n\t\t\t<\/div>\n\t\t\t\t<figcaption class='wp-caption-text gallery-caption' id='gallery-2-63'>\n\t\t\t\tHidetoshi KUMATA Ph.D<\/br>\nAssistant Prof.\n\t\t\t\t<\/figcaption><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon portrait'>\n\t\t\t\t<a href='https:\/\/www.ls.toyaku.ac.jp\/~bioanalchem\/en\/member\/aoki-120x150\/'><img loading=\"lazy\" decoding=\"async\" width=\"120\" height=\"150\" src=\"https:\/\/www.ls.toyaku.ac.jp\/~bioanalchem\/en\/wp-content\/uploads\/sites\/2\/2018\/07\/aoki-120x150-120x150.jpg\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\" aria-describedby=\"gallery-2-64\" \/><\/a>\n\t\t\t<\/div>\n\t\t\t\t<figcaption class='wp-caption-text gallery-caption' id='gallery-2-64'>\n\t\t\t\tMotohide AOKI, Ph.D<\/br>\nAssistant Prof.\n\t\t\t\t<\/figcaption><\/figure>\n\t\t<\/div>\n\n<div class='whatsnew'>\r\n\t\t\t<h1>What&#039;s New<\/h1>\t\r\n\t<hr\/>\r\n\t\t<dl>\r\n\t\t<a href=\"https:\/\/www.ls.toyaku.ac.jp\/~bioanalchem\/en\/2017\/09\/10\/best-poster-award\/\">\r\n\t\t<dt>\r\n\t\t\tSeptember 10, 2017\t\t<\/dt>\r\n\t\t<dd>\r\n\t\t\t\t\t\tRiko Takeuchi won the Best Poster Award !!\t\t<\/dd>\r\n\t\t<\/a>\r\n\t<\/dl>\r\n\t<hr\/>\r\n\t<\/div>\r\n\n<h1>Recent Pubrications<\/h1>\n<div class=\"teachpress_pub_list\"><form name=\"tppublistform\" method=\"get\"><a name=\"tppubs\" id=\"tppubs\"><\/a><\/form><div class=\"teachpress_publication_list\"><h3 class=\"tp_h3\" id=\"tp_h3_2022\">2022<\/h3><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\"> Ishii, Ryo;  Morioka, Kazuhiro;  Mizumoto, Takuya;  Yamasaki, Natsumi;  Hemmi, Akihide;  Shoji, Atsushi;  Murakami, Hiroya;  Teshima, Norio;  Umemura, Tomonari;  Uchiyama, Katsumi;  Nakajima, Hizuru<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('319','tp_links')\" style=\"cursor:pointer;\">Development of Portable Fluorescence Microplate Reader Equipped with Indium Tin Oxide Glass Heater for Loop-mediated Isothermal Amplification<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Sensors and Materials, <\/span><span class=\"tp_pub_additional_volume\">vol. 34, <\/span><span class=\"tp_pub_additional_pages\">pp. 1, <\/span><span class=\"tp_pub_additional_year\">2022<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_319\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('319','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_319\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('319','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_319\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{article,<br \/>\r\ntitle = {Development of Portable Fluorescence Microplate Reader Equipped with Indium Tin Oxide Glass Heater for Loop-mediated Isothermal Amplification},<br \/>\r\nauthor = {Ryo Ishii and Kazuhiro Morioka and Takuya Mizumoto and Natsumi Yamasaki and Akihide Hemmi and Atsushi Shoji and Hiroya Murakami and Norio Teshima and Tomonari Umemura and Katsumi Uchiyama and Hizuru Nakajima},<br \/>\r\ndoi = {10.18494\/SAM.2022.3618},<br \/>\r\nyear  = {2022},<br \/>\r\ndate = {2022-01-01},<br \/>\r\njournal = {Sensors and Materials},<br \/>\r\nvolume = {34},<br \/>\r\npages = {1},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('319','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_319\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/10.18494\/SAM.2022.3618\" title=\"Follow DOI:10.18494\/SAM.2022.3618\" target=\"_blank\">doi:10.18494\/SAM.2022.3618<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('319','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><\/div><\/div>\n<div class=\"teachpress_pub_list\"><form name=\"tppublistform\" method=\"get\"><a name=\"tppubs\" id=\"tppubs\"><\/a><\/form><div class=\"teachpress_publication_list\"><h3 class=\"tp_h3\" id=\"tp_h3_2021\">2021<\/h3><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\"> Miyauchi, Hiroki;  Harada, Kohei;  Suzuki, Yoshino;  Okada, Katsuhiko;  Aoki, Motohide;  Umemura, Tomonari;  Fujiwara, Shoko;  Tsuzuki, Mikio<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('320','tp_links')\" style=\"cursor:pointer;\">Development of an algal cell-attached solid surface culture system for simultaneous wastewater treatment and biomass production<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Algal Research, <\/span><span class=\"tp_pub_additional_volume\">vol. 58, <\/span><span class=\"tp_pub_additional_pages\">pp. 102394, <\/span><span class=\"tp_pub_additional_year\">2021<\/span>, <span class=\"tp_pub_additional_issn\">ISSN: 2211-9264<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_abstract_link\"><a id=\"tp_abstract_sh_320\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('320','tp_abstract')\" title=\"Show abstract\" style=\"cursor:pointer;\">Abstract<\/a><\/span> | <span class=\"tp_resource_link\"><a id=\"tp_links_sh_320\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('320','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_320\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('320','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_320\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{MIYAUCHI2021102394,<br \/>\r\ntitle = {Development of an algal cell-attached solid surface culture system for simultaneous wastewater treatment and biomass production},<br \/>\r\nauthor = {Hiroki Miyauchi and Kohei Harada and Yoshino Suzuki and Katsuhiko Okada and Motohide Aoki and Tomonari Umemura and Shoko Fujiwara and Mikio Tsuzuki},<br \/>\r\nurl = {https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2211926421002137},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1016\/j.algal.2021.102394},<br \/>\r\nissn = {2211-9264},<br \/>\r\nyear  = {2021},<br \/>\r\ndate = {2021-07-06},<br \/>\r\njournal = {Algal Research},<br \/>\r\nvolume = {58},<br \/>\r\npages = {102394},<br \/>\r\nabstract = {Wastewater treatment using microalgae is receiving growing attention. Here, we have developed a portable tubular system containing an algal cell-coated solid surface for phosphorous recovery. P-depleted Chlorella cells attached to a solid surface removed phosphate from the medium about 70 times faster than P-replete cells. When the cell density was 20\u00a0g\u00a0dry\u00a0cell\u00a0weight\u00a0m\u22122 or less, P-depleted cells on the solid surface absorbed phosphate from the medium at almost the same rate as in liquid, the maximum capacity per solid surface area being about 6\u00a0mg\u00a0P\u00a0m\u22122\u00a0min\u22121. P in inorganic wastewater from chemical factories (ethanol factories; about 4\u00a0mg\u00a0L\u22121) and in pond water (0.06\u00a0mg\u00a0L\u22121) was mostly removed within 3\u00a0h with a simple device composed of a single solid-surfaced sheet (0.002\u00a0m\u22122) and a portable compact tubular device including 12 such sheets (totally 0.5\u00a0m\u22122), respectively. Simultaneously, cellular growth was confirmed with both wastewater and pond water. These findings suggested that the attached cell culture system, in which medium exchange to prepare P-depleted cells is much easier than in liquid cultures, is promising for dual use for biomass production and wastewater treatment.},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('320','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_abstract\" id=\"tp_abstract_320\" style=\"display:none;\"><div class=\"tp_abstract_entry\">Wastewater treatment using microalgae is receiving growing attention. Here, we have developed a portable tubular system containing an algal cell-coated solid surface for phosphorous recovery. P-depleted Chlorella cells attached to a solid surface removed phosphate from the medium about 70 times faster than P-replete cells. When the cell density was 20\u00a0g\u00a0dry\u00a0cell\u00a0weight\u00a0m\u22122 or less, P-depleted cells on the solid surface absorbed phosphate from the medium at almost the same rate as in liquid, the maximum capacity per solid surface area being about 6\u00a0mg\u00a0P\u00a0m\u22122\u00a0min\u22121. P in inorganic wastewater from chemical factories (ethanol factories; about 4\u00a0mg\u00a0L\u22121) and in pond water (0.06\u00a0mg\u00a0L\u22121) was mostly removed within 3\u00a0h with a simple device composed of a single solid-surfaced sheet (0.002\u00a0m\u22122) and a portable compact tubular device including 12 such sheets (totally 0.5\u00a0m\u22122), respectively. Simultaneously, cellular growth was confirmed with both wastewater and pond water. These findings suggested that the attached cell culture system, in which medium exchange to prepare P-depleted cells is much easier than in liquid cultures, is promising for dual use for biomass production and wastewater treatment.<\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('320','tp_abstract')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_320\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2211926421002137\" title=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2211926421002137\" target=\"_blank\">https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2211926421002137<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1016\/j.algal.2021.102394\" title=\"Follow DOI:https:\/\/doi.org\/10.1016\/j.algal.2021.102394\" target=\"_blank\">doi:https:\/\/doi.org\/10.1016\/j.algal.2021.102394<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('320','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\"> Shoji, Atsushi;  Nakajima, Miyu;  Morioka, Kazuhiro;  Fujimori, Eiji;  Umemura, Tomonari;  Yanagida, Akio;  Hemmi, Akihide;  Uchiyama, Katsumi;  Nakajima, Hizuru<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('321','tp_links')\" style=\"cursor:pointer;\">Development of a surface plasmon resonance sensor using an optical fiber prepared by electroless displacement gold plating and its application to immunoassay<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Talanta, <\/span><span class=\"tp_pub_additional_volume\">vol. 240, <\/span><span class=\"tp_pub_additional_pages\">pp. 123162, <\/span><span class=\"tp_pub_additional_year\">2021<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_321\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('321','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_321\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('321','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_321\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{articleb,<br \/>\r\ntitle = {Development of a surface plasmon resonance sensor using an optical fiber prepared by electroless displacement gold plating and its application to immunoassay},<br \/>\r\nauthor = {Atsushi Shoji and Miyu Nakajima and Kazuhiro Morioka and Eiji Fujimori and Tomonari Umemura and Akio Yanagida and Akihide Hemmi and Katsumi Uchiyama and Hizuru Nakajima},<br \/>\r\ndoi = {10.1016\/j.talanta.2021.123162},<br \/>\r\nyear  = {2021},<br \/>\r\ndate = {2021-01-01},<br \/>\r\njournal = {Talanta},<br \/>\r\nvolume = {240},<br \/>\r\npages = {123162},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('321','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_321\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/10.1016\/j.talanta.2021.123162\" title=\"Follow DOI:10.1016\/j.talanta.2021.123162\" target=\"_blank\">doi:10.1016\/j.talanta.2021.123162<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('321','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\"> Morioka, Kazuhiro;  Osashima, Moeko;  Azuma, Nao;  Qu, Kuizhi;  Hemmi, Akihide;  Shoji, Atsushi;  Murakami, Hiroya;  Teshima, Norio;  Umemura, Tomonari;  Uchiyama, Katsumi;  Nakajima, Hizuru<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('322','tp_links')\" style=\"cursor:pointer;\">Development of a fluorescence microplate reader using an organic photodiode array with a large light receiving area<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Talanta, <\/span><span class=\"tp_pub_additional_volume\">vol. 238, <\/span><span class=\"tp_pub_additional_pages\">pp. 122994, <\/span><span class=\"tp_pub_additional_year\">2021<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_322\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('322','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_322\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('322','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_322\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{articlec,<br \/>\r\ntitle = {Development of a fluorescence microplate reader using an organic photodiode array with a large light receiving area},<br \/>\r\nauthor = {Kazuhiro Morioka and Moeko Osashima and Nao Azuma and Kuizhi Qu and Akihide Hemmi and Atsushi Shoji and Hiroya Murakami and Norio Teshima and Tomonari Umemura and Katsumi Uchiyama and Hizuru Nakajima},<br \/>\r\ndoi = {10.1016\/j.talanta.2021.122994},<br \/>\r\nyear  = {2021},<br \/>\r\ndate = {2021-01-01},<br \/>\r\njournal = {Talanta},<br \/>\r\nvolume = {238},<br \/>\r\npages = {122994},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('322','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_322\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/10.1016\/j.talanta.2021.122994\" title=\"Follow DOI:10.1016\/j.talanta.2021.122994\" target=\"_blank\">doi:10.1016\/j.talanta.2021.122994<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('322','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\"> MIKI, Yuta;  MURAKAMI, Hiroya;  IIDA, Keisuke;  UMEMURA, Tomonari;  ESAKA, Yukihiro;  INOUE, Yoshinori;  TESHIMA, Norio<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('323','tp_links')\" style=\"cursor:pointer;\">Preparation and Evaluation of Molding-type Solid-phase Extraction Media Binding with Commercially Available Adhesives<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Analytical Sciences, <\/span><span class=\"tp_pub_additional_year\">2021<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_323\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('323','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_323\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('323','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_323\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{articled,<br \/>\r\ntitle = {Preparation and Evaluation of Molding-type Solid-phase Extraction Media Binding with Commercially Available Adhesives},<br \/>\r\nauthor = {Yuta MIKI and Hiroya MURAKAMI and Keisuke IIDA and Tomonari UMEMURA and Yukihiro ESAKA and Yoshinori INOUE and Norio TESHIMA},<br \/>\r\ndoi = {10.2116\/analsci.21P265},<br \/>\r\nyear  = {2021},<br \/>\r\ndate = {2021-01-01},<br \/>\r\njournal = {Analytical Sciences},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('323','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_323\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/10.2116\/analsci.21P265\" title=\"Follow DOI:10.2116\/analsci.21P265\" target=\"_blank\">doi:10.2116\/analsci.21P265<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('323','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><\/div><\/div>\n<div class=\"teachpress_pub_list\"><form name=\"tppublistform\" method=\"get\"><a name=\"tppubs\" id=\"tppubs\"><\/a><\/form><div class=\"teachpress_publication_list\"><h3 class=\"tp_h3\" id=\"tp_h3_2020\">2020<\/h3><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\"> Matsuo, Yukiko;  Sasaki, Mayu;  Fukaya, Haruhiko;  Miyake, Katsunori;  Takeuchi, Riko;  Kumata, Hidetoshi;  Mimaki, Yoshihiro<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('324','tp_links')\" style=\"cursor:pointer;\">Chemical Constituents of the Terrestrial Stems of Ephedra sinica and their PPAR-&amp;gamma; Ligand-Binding Activity<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Planta Medica International Open, <\/span><span class=\"tp_pub_additional_volume\">vol. 7, <\/span><span class=\"tp_pub_additional_number\">no. 01, <\/span><span class=\"tp_pub_additional_pages\">pp. e12-e16, <\/span><span class=\"tp_pub_additional_year\">2020<\/span>, <span class=\"tp_pub_additional_issn\">ISSN: 2509-9264<\/span><span class=\"tp_pub_additional_note\">, (e12)<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_324\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('324','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_324\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('324','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_324\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Matsuo12.02.2020,<br \/>\r\ntitle = {Chemical Constituents of the Terrestrial Stems of Ephedra sinica and their PPAR-&gamma; Ligand-Binding Activity},<br \/>\r\nauthor = {Yukiko Matsuo and Mayu Sasaki and Haruhiko Fukaya and Katsunori Miyake and Riko Takeuchi and Hidetoshi Kumata and Yoshihiro Mimaki},<br \/>\r\nurl = {https:\/\/doi.org\/10.1055\/a-1094-9229},<br \/>\r\ndoi = {10.1055\/a-1094-9229},<br \/>\r\nissn = {2509-9264},<br \/>\r\nyear  = {2020},<br \/>\r\ndate = {2020-02-20},<br \/>\r\njournal = {Planta Medica International Open},<br \/>\r\nvolume = {7},<br \/>\r\nnumber = {01},<br \/>\r\npages = {e12-e16},<br \/>\r\nnote = {e12},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('324','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_324\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/doi.org\/10.1055\/a-1094-9229\" title=\"https:\/\/doi.org\/10.1055\/a-1094-9229\" target=\"_blank\">https:\/\/doi.org\/10.1055\/a-1094-9229<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/10.1055\/a-1094-9229\" title=\"Follow DOI:10.1055\/a-1094-9229\" target=\"_blank\">doi:10.1055\/a-1094-9229<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('324','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\"> Kishi, Takuho;  Kotani, Akira;  Umemura, Tomonari;  Hakamata, Hideki<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('325','tp_links')\" style=\"cursor:pointer;\">HPLC with electrochemical detection for determining homogentisic acid and its application to urine from rats fed tyrosine-enriched food<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Journal of Pharmaceutical and Biomedical Analysis, <\/span><span class=\"tp_pub_additional_volume\">vol. 186, <\/span><span class=\"tp_pub_additional_pages\">pp. 113253, <\/span><span class=\"tp_pub_additional_year\">2020<\/span>, <span class=\"tp_pub_additional_issn\">ISSN: 0731-7085<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_abstract_link\"><a id=\"tp_abstract_sh_325\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('325','tp_abstract')\" title=\"Show abstract\" style=\"cursor:pointer;\">Abstract<\/a><\/span> | <span class=\"tp_resource_link\"><a id=\"tp_links_sh_325\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('325','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_325\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('325','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_325\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{KISHI2020113253,<br \/>\r\ntitle = {HPLC with electrochemical detection for determining homogentisic acid and its application to urine from rats fed tyrosine-enriched food},<br \/>\r\nauthor = {Takuho Kishi and Akira Kotani and Tomonari Umemura and Hideki Hakamata},<br \/>\r\nurl = {http:\/\/www.sciencedirect.com\/science\/article\/pii\/S073170851932967X},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1016\/j.jpba.2020.113253},<br \/>\r\nissn = {0731-7085},<br \/>\r\nyear  = {2020},<br \/>\r\ndate = {2020-01-01},<br \/>\r\njournal = {Journal of Pharmaceutical and Biomedical Analysis},<br \/>\r\nvolume = {186},<br \/>\r\npages = {113253},<br \/>\r\nabstract = {A highly sensitive method for determining urine homogentisic acid (HGA) is required to provide adequate diagnosis and therapy for alkaptonuria in early stages. In this study, we developed a highly sensitive high-performance liquid chromatography with electrochemical detection (HPLC-ECD) for determining HGA in urine. In order to obtain a chromatogram of HGA by HPLC-ECD, an oxidation current was monitored at +0.5\u202fV vs. Ag\/AgCl. The peak heights of HGA showed linearity (r\u202f=\u202f0.999) ranging from 4.2\u202fng\/mL to 168\u202fng\/mL, and the detection limit was 1.2\u202fng\/mL (signal-to-noise ratio, S\/N\u202f=\u202f3). In recovery tests using human control urine spiked with an HGA standard, the recoveries of HGA were more than 93.2 %, and the relative standard deviations (n\u202f=\u202f6) were less than 1.9 %. As an in vivo application using male Wistar rats, the level of urine HGA, which was metabolized from tyrosine in tyrosine-enriched food, was determined by this HPLC-ECD method. The determination of HGA in urine by this HPLC-ECD method requires only 0.1\u202fmL of a rat urine specimen and simple sample preparation consisting of dilution and filtration.},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('325','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_abstract\" id=\"tp_abstract_325\" style=\"display:none;\"><div class=\"tp_abstract_entry\">A highly sensitive method for determining urine homogentisic acid (HGA) is required to provide adequate diagnosis and therapy for alkaptonuria in early stages. In this study, we developed a highly sensitive high-performance liquid chromatography with electrochemical detection (HPLC-ECD) for determining HGA in urine. In order to obtain a chromatogram of HGA by HPLC-ECD, an oxidation current was monitored at +0.5\u202fV vs. Ag\/AgCl. The peak heights of HGA showed linearity (r\u202f=\u202f0.999) ranging from 4.2\u202fng\/mL to 168\u202fng\/mL, and the detection limit was 1.2\u202fng\/mL (signal-to-noise ratio, S\/N\u202f=\u202f3). In recovery tests using human control urine spiked with an HGA standard, the recoveries of HGA were more than 93.2 %, and the relative standard deviations (n\u202f=\u202f6) were less than 1.9 %. As an in vivo application using male Wistar rats, the level of urine HGA, which was metabolized from tyrosine in tyrosine-enriched food, was determined by this HPLC-ECD method. The determination of HGA in urine by this HPLC-ECD method requires only 0.1\u202fmL of a rat urine specimen and simple sample preparation consisting of dilution and filtration.<\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('325','tp_abstract')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_325\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S073170851932967X\" title=\"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S073170851932967X\" target=\"_blank\">http:\/\/www.sciencedirect.com\/science\/article\/pii\/S073170851932967X<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1016\/j.jpba.2020.113253\" title=\"Follow DOI:https:\/\/doi.org\/10.1016\/j.jpba.2020.113253\" target=\"_blank\">doi:https:\/\/doi.org\/10.1016\/j.jpba.2020.113253<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('325','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\"> Endo, Satoshi;  Yoshimura, Mitsuki;  Kumata, Hidetoshi;  Uchida, Masao;  Yabuki, Yoshinori;  Nakata, Haruhiko<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('326','tp_links')\" style=\"cursor:pointer;\">Reduced bioavailability of polycyclic aromatic hydrocarbons (PAHs) in sediments impacted by carbon manufacturing plant effluent: Evaluation by ex situ passive sampling method<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Environmental Pollution, <\/span><span class=\"tp_pub_additional_volume\">vol. 256, <\/span><span class=\"tp_pub_additional_pages\">pp. 113448, <\/span><span class=\"tp_pub_additional_year\">2020<\/span>, <span class=\"tp_pub_additional_issn\">ISSN: 0269-7491<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_abstract_link\"><a id=\"tp_abstract_sh_326\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('326','tp_abstract')\" title=\"Show abstract\" style=\"cursor:pointer;\">Abstract<\/a><\/span> | <span class=\"tp_resource_link\"><a id=\"tp_links_sh_326\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('326','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_326\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('326','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_326\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{ENDO2020113448,<br \/>\r\ntitle = {Reduced bioavailability of polycyclic aromatic hydrocarbons (PAHs) in sediments impacted by carbon manufacturing plant effluent: Evaluation by ex situ passive sampling method},<br \/>\r\nauthor = {Satoshi Endo and Mitsuki Yoshimura and Hidetoshi Kumata and Masao Uchida and Yoshinori Yabuki and Haruhiko Nakata},<br \/>\r\nurl = {http:\/\/www.sciencedirect.com\/science\/article\/pii\/S0269749119349796},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1016\/j.envpol.2019.113448},<br \/>\r\nissn = {0269-7491},<br \/>\r\nyear  = {2020},<br \/>\r\ndate = {2020-01-01},<br \/>\r\njournal = {Environmental Pollution},<br \/>\r\nvolume = {256},<br \/>\r\npages = {113448},<br \/>\r\nabstract = {Potential risks of polycyclic aromatic hydrocarbons (PAHs) in sediments of a Japanese bay contaminated by carbon manufacturing plant effluent were evaluated by calculating toxicity units (TUs). TUs calculated from the measured whole-sediment concentrations (Cwhole) were often higher than or close to 1, signaling a possible toxicity concern to benthic organisms. In contrast, TUs based on the freely dissolved pore water concentrations (Cfree) measured by an ex-situ passive sampling method with polyethylene strips were 0.0007\u20130.005, much lower than 1, indicating no effect. We also found that the fractions of black carbon in sediments of the contaminated bay were significantly higher than those of reference sites. Overall, we conclude that carbon manufacturing plant effluent substantially increases Cwhole of PAHs in sediments but also increases the fraction of carbonaceous particles that strongly retain PAHs. As a combined result, bioavailable concentrations (as expressed by pore water Cfree) of PAHs do not increase as much as Cwhole. The results of this study indicate that ecotoxicological risks of PAH contamination by carbon manufacturing plants should be evaluated by directly measuring pore water Cfree instead of Cwhole.},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('326','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_abstract\" id=\"tp_abstract_326\" style=\"display:none;\"><div class=\"tp_abstract_entry\">Potential risks of polycyclic aromatic hydrocarbons (PAHs) in sediments of a Japanese bay contaminated by carbon manufacturing plant effluent were evaluated by calculating toxicity units (TUs). TUs calculated from the measured whole-sediment concentrations (Cwhole) were often higher than or close to 1, signaling a possible toxicity concern to benthic organisms. In contrast, TUs based on the freely dissolved pore water concentrations (Cfree) measured by an ex-situ passive sampling method with polyethylene strips were 0.0007\u20130.005, much lower than 1, indicating no effect. We also found that the fractions of black carbon in sediments of the contaminated bay were significantly higher than those of reference sites. Overall, we conclude that carbon manufacturing plant effluent substantially increases Cwhole of PAHs in sediments but also increases the fraction of carbonaceous particles that strongly retain PAHs. As a combined result, bioavailable concentrations (as expressed by pore water Cfree) of PAHs do not increase as much as Cwhole. The results of this study indicate that ecotoxicological risks of PAH contamination by carbon manufacturing plants should be evaluated by directly measuring pore water Cfree instead of Cwhole.<\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('326','tp_abstract')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_326\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S0269749119349796\" title=\"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S0269749119349796\" target=\"_blank\">http:\/\/www.sciencedirect.com\/science\/article\/pii\/S0269749119349796<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1016\/j.envpol.2019.113448\" title=\"Follow DOI:https:\/\/doi.org\/10.1016\/j.envpol.2019.113448\" target=\"_blank\">doi:https:\/\/doi.org\/10.1016\/j.envpol.2019.113448<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('326','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\"> \u66f2, \u594e\u667a;  \u68ee\u5ca1, \u548c\u5927;  \u6771, \u5948\u7a57;  \u9577\u5d8b, \u840c\u5b50;  \u8fba\u898b, \u5f70\u79c0;  \u6771\u6d77\u6797, \u6566;  \u6751\u4e0a, \u535a\u54c9;  \u624b\u5d8b, \u7d00\u96c4;  \u6885\u6751, \u77e5\u4e5f;  \u52a0\u85e4, \u4fca\u543e;  \u6cb3\u897f, \u5948\u4fdd\u5b50;  \u5185\u5c71, \u4e00\u7f8e;  \u4e2d\u5d8b, \u79c0<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('327','tp_links')\" style=\"cursor:pointer;\">\u81ea\u5f8b\u9001\u6db2\u304c\u53ef\u80fd\u306a\u30de\u30a4\u30af\u30ed\u30c1\u30c3\u30d7\u3068\u6709\u6a5f\u30d5\u30a9\u30c8\u30c0\u30a4\u30aa\u30fc\u30c9\u691c\u51fa\u5668\u3092\u7528\u3044\u308b\u5316\u5b66\u767a\u5149\u5206\u6790\u30b7\u30b9\u30c6\u30e0\u306e\u958b\u767a<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">\u5206\u6790\u5316\u5b66, <\/span><span class=\"tp_pub_additional_volume\">vol. 69, <\/span><span class=\"tp_pub_additional_number\">no. 1.2, <\/span><span class=\"tp_pub_additional_pages\">pp. 31-39, <\/span><span class=\"tp_pub_additional_year\">2020<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_327\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('327','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_327\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('327','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_327\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{\u66f2\u594e\u667a2020,<br \/>\r\ntitle = {\u81ea\u5f8b\u9001\u6db2\u304c\u53ef\u80fd\u306a\u30de\u30a4\u30af\u30ed\u30c1\u30c3\u30d7\u3068\u6709\u6a5f\u30d5\u30a9\u30c8\u30c0\u30a4\u30aa\u30fc\u30c9\u691c\u51fa\u5668\u3092\u7528\u3044\u308b\u5316\u5b66\u767a\u5149\u5206\u6790\u30b7\u30b9\u30c6\u30e0\u306e\u958b\u767a},<br \/>\r\nauthor = {\u594e\u667a \u66f2 and \u548c\u5927 \u68ee\u5ca1 and \u5948\u7a57 \u6771 and \u840c\u5b50 \u9577\u5d8b and \u5f70\u79c0 \u8fba\u898b and \u6566 \u6771\u6d77\u6797 and \u535a\u54c9 \u6751\u4e0a and \u7d00\u96c4 \u624b\u5d8b and \u77e5\u4e5f \u6885\u6751 and \u4fca\u543e \u52a0\u85e4 and \u5948\u4fdd\u5b50 \u6cb3\u897f and \u4e00\u7f8e \u5185\u5c71 and \u79c0 \u4e2d\u5d8b},<br \/>\r\ndoi = {10.2116\/bunsekikagaku.69.31},<br \/>\r\nyear  = {2020},<br \/>\r\ndate = {2020-01-01},<br \/>\r\njournal = {\u5206\u6790\u5316\u5b66},<br \/>\r\nvolume = {69},<br \/>\r\nnumber = {1.2},<br \/>\r\npages = {31-39},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('327','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_327\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/10.2116\/bunsekikagaku.69.31\" title=\"Follow DOI:10.2116\/bunsekikagaku.69.31\" target=\"_blank\">doi:10.2116\/bunsekikagaku.69.31<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('327','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\"> Murakami, Hiroya;  Omiya, Miho;  Miki, Yuta;  Umemura, Tomonari;  Esaka, Yukihiro;  Inoue, Yoshinori;  Teshima, Norio<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('328','tp_links')\" style=\"cursor:pointer;\">Evaluation of the adsorption properties of nucleobase-modified sorbents for a solid-phase extraction of water-soluble compounds<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Talanta, <\/span><span class=\"tp_pub_additional_volume\">vol. 217, <\/span><span class=\"tp_pub_additional_pages\">pp. 121052, <\/span><span class=\"tp_pub_additional_year\">2020<\/span>, <span class=\"tp_pub_additional_issn\">ISSN: 0039-9140<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_abstract_link\"><a id=\"tp_abstract_sh_328\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('328','tp_abstract')\" title=\"Show abstract\" style=\"cursor:pointer;\">Abstract<\/a><\/span> | <span class=\"tp_resource_link\"><a id=\"tp_links_sh_328\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('328','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_328\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('328','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_328\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{MURAKAMI2020121052,<br \/>\r\ntitle = {Evaluation of the adsorption properties of nucleobase-modified sorbents for a solid-phase extraction of water-soluble compounds},<br \/>\r\nauthor = {Hiroya Murakami and Miho Omiya and Yuta Miki and Tomonari Umemura and Yukihiro Esaka and Yoshinori Inoue and Norio Teshima},<br \/>\r\nurl = {http:\/\/www.sciencedirect.com\/science\/article\/pii\/S003991402030343X},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1016\/j.talanta.2020.121052},<br \/>\r\nissn = {0039-9140},<br \/>\r\nyear  = {2020},<br \/>\r\ndate = {2020-01-01},<br \/>\r\njournal = {Talanta},<br \/>\r\nvolume = {217},<br \/>\r\npages = {121052},<br \/>\r\nabstract = {We developed hydrophilic interaction chromatography (HILIC)-type sorbents modified with nucleobases for solid phase extraction (SPE). The synthesized hydrophilic base resins were modified by each nucleobase (adenine, guanine, and cytosine). The measurement of the amount of water content indicated that each nucleobase-modified sorbent had a water layer. To evaluate the adsorption properties in the HILIC mode, we chose two nucleobases (uracil and adenine) and four nucleosides (uridine, adenosine, cytidine, guanosine) as water-soluble analytes, which were loaded into an SPE cartridge packed with the nucleobase-modified sorbent. Firstly, 95% acetonitrile (ACN) solutions were used in the process of conditioning and sample loading of the above polar analytes. High recoveries of the analytes were observed in each nucleobase-modified sorbent, and the Diol-type sorbent (no modification with any of the nucleobases) did not adsorb each water-soluble analyte. On the basis of this result, a 98% ACN solution was used during the process of conditioning and sample loading to decrease the concentration of water in the sample, which potentially inhibited the formation of hydrogen bonding between each analyte and the modified nucleobase. Considerable improvements of recoveries were observed in Adenine- and Cytosine-modified sorbents. These results were possibly attributed to the effective expression of hydrogen bonding by decreasing water concentration in the sample solution. Although a non-aqueous (100% ACN) sample solution can be expected to obtain higher recoveries compared with the 98% ACN solution, a decrease in recoveries was observed in Adenine-modified sorbent. From these results, the highest adsorption property was observed in Adenine-modified sorbent using 98% ACN as a sample condition, and the combination of this sample condition and sorbent is effective for high adsorption under HILIC condition. Moreover, we also revealed that a balance between the thickness of water layer and the modification amount of nucleobase is important for retention in the HILIC-type sorbent.},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('328','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_abstract\" id=\"tp_abstract_328\" style=\"display:none;\"><div class=\"tp_abstract_entry\">We developed hydrophilic interaction chromatography (HILIC)-type sorbents modified with nucleobases for solid phase extraction (SPE). The synthesized hydrophilic base resins were modified by each nucleobase (adenine, guanine, and cytosine). The measurement of the amount of water content indicated that each nucleobase-modified sorbent had a water layer. To evaluate the adsorption properties in the HILIC mode, we chose two nucleobases (uracil and adenine) and four nucleosides (uridine, adenosine, cytidine, guanosine) as water-soluble analytes, which were loaded into an SPE cartridge packed with the nucleobase-modified sorbent. Firstly, 95% acetonitrile (ACN) solutions were used in the process of conditioning and sample loading of the above polar analytes. High recoveries of the analytes were observed in each nucleobase-modified sorbent, and the Diol-type sorbent (no modification with any of the nucleobases) did not adsorb each water-soluble analyte. On the basis of this result, a 98% ACN solution was used during the process of conditioning and sample loading to decrease the concentration of water in the sample, which potentially inhibited the formation of hydrogen bonding between each analyte and the modified nucleobase. Considerable improvements of recoveries were observed in Adenine- and Cytosine-modified sorbents. These results were possibly attributed to the effective expression of hydrogen bonding by decreasing water concentration in the sample solution. Although a non-aqueous (100% ACN) sample solution can be expected to obtain higher recoveries compared with the 98% ACN solution, a decrease in recoveries was observed in Adenine-modified sorbent. From these results, the highest adsorption property was observed in Adenine-modified sorbent using 98% ACN as a sample condition, and the combination of this sample condition and sorbent is effective for high adsorption under HILIC condition. Moreover, we also revealed that a balance between the thickness of water layer and the modification amount of nucleobase is important for retention in the HILIC-type sorbent.<\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('328','tp_abstract')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_328\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S003991402030343X\" title=\"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S003991402030343X\" target=\"_blank\">http:\/\/www.sciencedirect.com\/science\/article\/pii\/S003991402030343X<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1016\/j.talanta.2020.121052\" title=\"Follow DOI:https:\/\/doi.org\/10.1016\/j.talanta.2020.121052\" target=\"_blank\">doi:https:\/\/doi.org\/10.1016\/j.talanta.2020.121052<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('328','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\"> Kumata, Hidetoshi;  Morimoto, Chika;  Horie, Akihiro;  Tanabe, Akane;  Fujimori, Eiji;  Umemura, Tomonari<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('329','tp_links')\" style=\"cursor:pointer;\">Elimination of interfering molybdenum oxyanion with an anion-exchange monolithic spin tip (AXTip) for precise determination of cadmium in human urine by ICP-MS<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Talanta Open, <\/span><span class=\"tp_pub_additional_pages\">pp. 100009, <\/span><span class=\"tp_pub_additional_year\">2020<\/span>, <span class=\"tp_pub_additional_issn\">ISSN: 2666-8319<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_abstract_link\"><a id=\"tp_abstract_sh_329\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('329','tp_abstract')\" title=\"Show abstract\" style=\"cursor:pointer;\">Abstract<\/a><\/span> | <span class=\"tp_resource_link\"><a id=\"tp_links_sh_329\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('329','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_329\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('329','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_329\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{KUMATA2020100009,<br \/>\r\ntitle = {Elimination of interfering molybdenum oxyanion with an anion-exchange monolithic spin tip (AXTip) for precise determination of cadmium in human urine by ICP-MS},<br \/>\r\nauthor = {Hidetoshi Kumata and Chika Morimoto and Akihiro Horie and Akane Tanabe and Eiji Fujimori and Tomonari Umemura},<br \/>\r\nurl = {http:\/\/www.sciencedirect.com\/science\/article\/pii\/S2666831920300096},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1016\/j.talo.2020.100009},<br \/>\r\nissn = {2666-8319},<br \/>\r\nyear  = {2020},<br \/>\r\ndate = {2020-01-01},<br \/>\r\njournal = {Talanta Open},<br \/>\r\npages = {100009},<br \/>\r\nabstract = {A simple purification technique using an anion-exchange monolithic spin tip was described for the elimination of molybdenum oxyanion interfering with precise determination of cadmium in human urine samples by ICP-MS. The tip device named \u201canion-exchange tip-in monolith (AXTip)\u201d was laboratory-prepared within the confines of a commercially available syringe filter tip by a two-step process: (1) in situ copolymerization of glycidyl methacrylate (GMA) and ethylene dimethacrylate (EDMA) and (2) its subsequent modification with trimethylamine (TMA), as a strong anion-exchange functional group, via ring-opening reaction of epoxide. The adsorption capacity of the AXTip was &gt;3.2 \u03bcmol\/tip for molybdenum oxyanion. The performance of the AXTip was evaluated through an adsorption\/non-adsorption experiment for molybdenum, cadmium and other metal and metalloid species, using a model solution and human urine samples to optimize the sample solution's pH and dilution factor for the AXTip treatment. In the case of urine sample analysis, 8 times dilution and pH adjustment to 4.0 prior to AXTip treatment were found to allow &gt;98 % elimination of interfering molybdenum oxyanions and quantitative recovery (103\u00b17 %) of cadmium. At the optimized conditions, the AXTip treatment reduces Mo\/Cd ratio from \u223c400 in human urines to &lt;10, which is sufficient for conducting ICP-MS measurement of cadmium in no-gas mode (without collision\/reaction cell technique). The proposed purification technique was validated through the analysis of an accuracy control material of human urine (Seronorm\u2122 Trace Elements Urine L-1). Good agreement of the observed values with the reference values indicates that the proposed technique is practically applicable.},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('329','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_abstract\" id=\"tp_abstract_329\" style=\"display:none;\"><div class=\"tp_abstract_entry\">A simple purification technique using an anion-exchange monolithic spin tip was described for the elimination of molybdenum oxyanion interfering with precise determination of cadmium in human urine samples by ICP-MS. The tip device named \u201canion-exchange tip-in monolith (AXTip)\u201d was laboratory-prepared within the confines of a commercially available syringe filter tip by a two-step process: (1) in situ copolymerization of glycidyl methacrylate (GMA) and ethylene dimethacrylate (EDMA) and (2) its subsequent modification with trimethylamine (TMA), as a strong anion-exchange functional group, via ring-opening reaction of epoxide. The adsorption capacity of the AXTip was &gt;3.2 \u03bcmol\/tip for molybdenum oxyanion. The performance of the AXTip was evaluated through an adsorption\/non-adsorption experiment for molybdenum, cadmium and other metal and metalloid species, using a model solution and human urine samples to optimize the sample solution's pH and dilution factor for the AXTip treatment. In the case of urine sample analysis, 8 times dilution and pH adjustment to 4.0 prior to AXTip treatment were found to allow &gt;98 % elimination of interfering molybdenum oxyanions and quantitative recovery (103\u00b17 %) of cadmium. At the optimized conditions, the AXTip treatment reduces Mo\/Cd ratio from \u223c400 in human urines to &lt;10, which is sufficient for conducting ICP-MS measurement of cadmium in no-gas mode (without collision\/reaction cell technique). The proposed purification technique was validated through the analysis of an accuracy control material of human urine (Seronorm\u2122 Trace Elements Urine L-1). Good agreement of the observed values with the reference values indicates that the proposed technique is practically applicable.<\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('329','tp_abstract')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_329\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S2666831920300096\" title=\"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S2666831920300096\" target=\"_blank\">http:\/\/www.sciencedirect.com\/science\/article\/pii\/S2666831920300096<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1016\/j.talo.2020.100009\" title=\"Follow DOI:https:\/\/doi.org\/10.1016\/j.talo.2020.100009\" target=\"_blank\">doi:https:\/\/doi.org\/10.1016\/j.talo.2020.100009<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('329','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\"> \u4eca\u5d0e, \u9f8d\u4e4b\u4ecb;  \u8fd1\u85e4, \u5553\u592a;  \u8c37, \u590f\u6d77;  \u9752\u6728, \u5143\u79c0;  \u718a\u7530, \u82f1\u5cf0;  \u5185\u7530, \u9054\u4e5f;  \u9577\u7e04, \u8c6a;  \u5d8b\u7530, \u6cf0\u4f51;  \u7530\u53e3, \u5609\u5f66;  \u4f50\u85e4, \u6d69\u660e;  \u5b89\u4e95, \u9686\u96c4;  \u6885\u6751, \u77e5\u4e5f<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('330','tp_links')\" style=\"cursor:pointer;\">\u8d85\u8584\u5c64\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u7528\u306e\u6c34\u5e73\u5f0f\u30df\u30cb\u30c1\u30e5\u30a2TLC\u5c55\u958b\u69fd\u306e\u8a66\u4f5c\u3068\u8a55\u4fa1<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">\u5206\u6790\u5316\u5b66, <\/span><span class=\"tp_pub_additional_volume\">vol. 69, <\/span><span class=\"tp_pub_additional_number\">no. 10.11, <\/span><span class=\"tp_pub_additional_pages\">pp. 553-558, <\/span><span class=\"tp_pub_additional_year\">2020<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_330\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('330','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_330\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('330','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_330\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{\u4eca\u5d0e\u9f8d\u4e4b\u4ecb2020,<br \/>\r\ntitle = {\u8d85\u8584\u5c64\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u7528\u306e\u6c34\u5e73\u5f0f\u30df\u30cb\u30c1\u30e5\u30a2TLC\u5c55\u958b\u69fd\u306e\u8a66\u4f5c\u3068\u8a55\u4fa1},<br \/>\r\nauthor = {\u9f8d\u4e4b\u4ecb \u4eca\u5d0e and \u5553\u592a \u8fd1\u85e4 and \u590f\u6d77 \u8c37 and \u5143\u79c0 \u9752\u6728 and \u82f1\u5cf0 \u718a\u7530 and \u9054\u4e5f \u5185\u7530 and \u8c6a \u9577\u7e04 and \u6cf0\u4f51 \u5d8b\u7530 and \u5609\u5f66 \u7530\u53e3 and \u6d69\u660e \u4f50\u85e4 and \u9686\u96c4 \u5b89\u4e95 and \u77e5\u4e5f \u6885\u6751},<br \/>\r\ndoi = {10.2116\/bunsekikagaku.69.553},<br \/>\r\nyear  = {2020},<br \/>\r\ndate = {2020-01-01},<br \/>\r\njournal = {\u5206\u6790\u5316\u5b66},<br \/>\r\nvolume = {69},<br \/>\r\nnumber = {10.11},<br \/>\r\npages = {553-558},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('330','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_330\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/10.2116\/bunsekikagaku.69.553\" title=\"Follow DOI:10.2116\/bunsekikagaku.69.553\" target=\"_blank\">doi:10.2116\/bunsekikagaku.69.553<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('330','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\"> Wakana, Yuichi;  Hayashi, Kaito;  Nemoto, Takumi;  Watanabe, Chiaki;  Taoka, Masato;  Angulo-Capel, Jessica;  Parajo, Maria;  Kumata, Hidetoshi;  Umemura, Tomonari;  Inoue, Hiroki;  Arasaki, Kohei;  Campelo, Felix;  Tagaya, Mitsuo<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('331','tp_links')\" style=\"cursor:pointer;\">The ER cholesterol sensor SCAP promotes CARTS biogenesis at ER\u2013Golgi membrane contact sites<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Journal of Cell Biology, <\/span><span class=\"tp_pub_additional_volume\">vol. 220, <\/span><span class=\"tp_pub_additional_year\">2020<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_331\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('331','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_331\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('331','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_331\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{articlef,<br \/>\r\ntitle = {The ER cholesterol sensor SCAP promotes CARTS biogenesis at ER\u2013Golgi membrane contact sites},<br \/>\r\nauthor = {Yuichi Wakana and Kaito Hayashi and Takumi Nemoto and Chiaki Watanabe and Masato Taoka and Jessica Angulo-Capel and Maria Parajo and Hidetoshi Kumata and Tomonari Umemura and Hiroki Inoue and Kohei Arasaki and Felix Campelo and Mitsuo Tagaya},<br \/>\r\ndoi = {10.1083\/jcb.202002150},<br \/>\r\nyear  = {2020},<br \/>\r\ndate = {2020-01-01},<br \/>\r\njournal = {Journal of Cell Biology},<br \/>\r\nvolume = {220},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('331','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_331\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/10.1083\/jcb.202002150\" title=\"Follow DOI:10.1083\/jcb.202002150\" target=\"_blank\">doi:10.1083\/jcb.202002150<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('331','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><\/div><\/div>\n<div class=\"teachpress_pub_list\"><form name=\"tppublistform\" method=\"get\"><a name=\"tppubs\" id=\"tppubs\"><\/a><\/form><div class=\"teachpress_publication_list\"><h3 class=\"tp_h3\" id=\"tp_h3_2019\">2019<\/h3><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\"> Tani, Hidenori;  Numajiri, Ayaka;  Aoki, Motohide;  Umemura, Tomonari;  Nakazato, Tetsuya<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('332','tp_links')\" style=\"cursor:pointer;\">Short-lived long noncoding RNAs as surrogate indicators for chemical stress in HepG2 cells and their degradation by nuclear RNases<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Scientific Reports, <\/span><span class=\"tp_pub_additional_volume\">vol. 9, <\/span><span class=\"tp_pub_additional_pages\">pp. 20299, <\/span><span class=\"tp_pub_additional_year\">2019<\/span>, <span class=\"tp_pub_additional_issn\">ISSN: 2045-2322<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_abstract_link\"><a id=\"tp_abstract_sh_332\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('332','tp_abstract')\" title=\"Show abstract\" style=\"cursor:pointer;\">Abstract<\/a><\/span> | <span class=\"tp_resource_link\"><a id=\"tp_links_sh_332\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('332','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_332\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('332','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_332\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Tani2019,<br \/>\r\ntitle = {Short-lived long noncoding RNAs as surrogate indicators for chemical stress in HepG2 cells and their degradation by nuclear RNases},<br \/>\r\nauthor = {Hidenori Tani and Ayaka Numajiri and Motohide Aoki and Tomonari Umemura and Tetsuya Nakazato},<br \/>\r\nurl = {www.nature.com\/scientificreports},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1038\/s41598-019-56869-y},<br \/>\r\nissn = {2045-2322},<br \/>\r\nyear  = {2019},<br \/>\r\ndate = {2019-12-30},<br \/>\r\njournal = {Scientific Reports},<br \/>\r\nvolume = {9},<br \/>\r\npages = {20299},<br \/>\r\nabstract = {Long noncoding RNAs (lncRNAs) are non-protein-coding transcripts &gt;200 nucleotides in length that have been shown to play important roles in various biological processes. The mechanisms underlying the induction of lncRNA expression by chemical exposure remain to be determined. We identified a novel class of short-lived lncRNAs with half-lives (t1\/2) \u22644\u2009hours in human HeLa Tet-off cells, which have been suggested to express many lncRNAs with regulatory functions. As they may affect various human biological processes, short-lived lncRNAs may be useful indicators of the degree of stress on chemical exposure. In the present study, we identified four short-lived lncRNAs, designated as OIP5-AS1, FLJ46906, LINC01137, and GABPB1-AS1, which showed significantly upregulated expression following exposure to hydrogen peroxide (oxidative stress), mercury II chloride (heavy metal stress), and etoposide (DNA damage stress) in human HepG2 cells. These lncRNAs may be useful indicators of chemical stress responses. The levels of these lncRNAs in the cells were increased because of chemical stress-induced prolongation of their decay. These lncRNAs were degraded by nuclear RNases, which are components of the exosome and XRN2, and chemical exposure inhibited the RNase activities within the cells.},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('332','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_abstract\" id=\"tp_abstract_332\" style=\"display:none;\"><div class=\"tp_abstract_entry\">Long noncoding RNAs (lncRNAs) are non-protein-coding transcripts &gt;200 nucleotides in length that have been shown to play important roles in various biological processes. The mechanisms underlying the induction of lncRNA expression by chemical exposure remain to be determined. We identified a novel class of short-lived lncRNAs with half-lives (t1\/2) \u22644\u2009hours in human HeLa Tet-off cells, which have been suggested to express many lncRNAs with regulatory functions. As they may affect various human biological processes, short-lived lncRNAs may be useful indicators of the degree of stress on chemical exposure. In the present study, we identified four short-lived lncRNAs, designated as OIP5-AS1, FLJ46906, LINC01137, and GABPB1-AS1, which showed significantly upregulated expression following exposure to hydrogen peroxide (oxidative stress), mercury II chloride (heavy metal stress), and etoposide (DNA damage stress) in human HepG2 cells. These lncRNAs may be useful indicators of chemical stress responses. The levels of these lncRNAs in the cells were increased because of chemical stress-induced prolongation of their decay. These lncRNAs were degraded by nuclear RNases, which are components of the exosome and XRN2, and chemical exposure inhibited the RNase activities within the cells.<\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('332','tp_abstract')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_332\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"www.nature.com\/scientificreports\" title=\"www.nature.com\/scientificreports\" target=\"_blank\">www.nature.com\/scientificreports<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1038\/s41598-019-56869-y\" title=\"Follow DOI:https:\/\/doi.org\/10.1038\/s41598-019-56869-y\" target=\"_blank\">doi:https:\/\/doi.org\/10.1038\/s41598-019-56869-y<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('332','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\"> Umemura, Tomonari<\/p><p class=\"tp_pub_title\">Elucidation of Ion-Pairing Elution Behavior of Anions and Cations in Electrostatic Ion Chromatography Using Water as a Mobile Phase <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Chromatography, <\/span><span class=\"tp_pub_additional_volume\">vol. 40, <\/span><span class=\"tp_pub_additional_pages\">pp. 143-148, <\/span><span class=\"tp_pub_additional_year\">2019<\/span>, <span class=\"tp_pub_additional_issn\">ISSN: 1342-8284<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_333\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('333','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_333\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Umemura2019100101,<br \/>\r\ntitle = {Elucidation of Ion-Pairing Elution Behavior of Anions and Cations in Electrostatic Ion Chromatography Using Water as a Mobile Phase},<br \/>\r\nauthor = {Tomonari Umemura},<br \/>\r\nissn = {1342-8284},<br \/>\r\nyear  = {2019},<br \/>\r\ndate = {2019-10-01},<br \/>\r\njournal = {Chromatography},<br \/>\r\nvolume = {40},<br \/>\r\npages = {143-148},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('333','tp_bibtex')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\"> \u7af9\u5185, \u7406\u5b50;  \u529b\u77f3, \u5609\u4eba;  \u5c0f\u5ddd, \u5948\u3005\u5b50;  \u98a8\u5442\u7530, \u90f7\u53f2;  \u5927\u6cb3\u5185, \u76f4\u5f66;  \u9752\u6728, \u5143\u79c0;  \u5185\u7530, \u9054\u4e5f;  \u6885\u6751, \u77e5\u4e5f;  \u718a\u7530, \u82f1\u5cf0<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('334','tp_links')\" style=\"cursor:pointer;\">\u5316\u5408\u7269\u30ec\u30d9\u30eb\u70ad\u7d20\u5b89\u5b9a\u540c\u4f4d\u4f53\u6bd4\u5206\u6790\u306e\u305f\u3081\u306e\u9ad8\u7b49\u690d\u7269\u4e2d\u306e\u30b9\u30c6\u30ed\u30fc\u30eb\u53ca\u3073\u8102\u80aa\u9178\u306e\u5206\u96e2\u7cbe\u88fd\u6cd5\u306e\u78ba\u7acb<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">\u5206\u6790\u5316\u5b66, <\/span><span class=\"tp_pub_additional_volume\">vol. 68, <\/span><span class=\"tp_pub_additional_number\">no. 5, <\/span><span class=\"tp_pub_additional_pages\">pp. 297-306, <\/span><span class=\"tp_pub_additional_year\">2019<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_334\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('334','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_334\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('334','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_334\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{\u7af9\u5185\u7406\u5b502019,<br \/>\r\ntitle = {\u5316\u5408\u7269\u30ec\u30d9\u30eb\u70ad\u7d20\u5b89\u5b9a\u540c\u4f4d\u4f53\u6bd4\u5206\u6790\u306e\u305f\u3081\u306e\u9ad8\u7b49\u690d\u7269\u4e2d\u306e\u30b9\u30c6\u30ed\u30fc\u30eb\u53ca\u3073\u8102\u80aa\u9178\u306e\u5206\u96e2\u7cbe\u88fd\u6cd5\u306e\u78ba\u7acb},<br \/>\r\nauthor = {\u7406\u5b50 \u7af9\u5185 and \u5609\u4eba \u529b\u77f3 and \u5948\u3005\u5b50 \u5c0f\u5ddd and \u90f7\u53f2 \u98a8\u5442\u7530 and \u76f4\u5f66 \u5927\u6cb3\u5185 and \u5143\u79c0 \u9752\u6728 and \u9054\u4e5f \u5185\u7530 and \u77e5\u4e5f \u6885\u6751 and \u82f1\u5cf0 \u718a\u7530},<br \/>\r\ndoi = {10.2116\/bunsekikagaku.68.297},<br \/>\r\nyear  = {2019},<br \/>\r\ndate = {2019-04-01},<br \/>\r\njournal = {\u5206\u6790\u5316\u5b66},<br \/>\r\nvolume = {68},<br \/>\r\nnumber = {5},<br \/>\r\npages = {297-306},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('334','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_334\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/10.2116\/bunsekikagaku.68.297\" title=\"Follow DOI:10.2116\/bunsekikagaku.68.297\" target=\"_blank\">doi:10.2116\/bunsekikagaku.68.297<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('334','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\"> Murota, Chisato;  Fujiwara, Shoko;  Tsujishita, Maki;  Urabe, Kanae;  Takayanagi, Shuta;  Aoki, Motohide;  Umemura, Tomonari;  Eaton-Rye, Julian J;  Pitt, Frances D;  Tsuzuki, Mikio<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('335','tp_links')\" style=\"cursor:pointer;\">Hyper-resistance to arsenate in the cyanobacterium Synechocystis sp. PCC 6803 is influenced by the differential kinetics of its pst-ABC transporters and external phosphate concentration exposure<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Algal Research, <\/span><span class=\"tp_pub_additional_volume\">vol. 38, <\/span><span class=\"tp_pub_additional_pages\">pp. 101410, <\/span><span class=\"tp_pub_additional_year\">2019<\/span>, <span class=\"tp_pub_additional_issn\">ISSN: 2211-9264<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_abstract_link\"><a id=\"tp_abstract_sh_335\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('335','tp_abstract')\" title=\"Show abstract\" style=\"cursor:pointer;\">Abstract<\/a><\/span> | <span class=\"tp_resource_link\"><a id=\"tp_links_sh_335\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('335','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_335\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('335','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_335\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{MUROTA2019101410,<br \/>\r\ntitle = {Hyper-resistance to arsenate in the cyanobacterium Synechocystis sp. PCC 6803 is influenced by the differential kinetics of its pst-ABC transporters and external phosphate concentration exposure},<br \/>\r\nauthor = {Chisato Murota and Shoko Fujiwara and Maki Tsujishita and Kanae Urabe and Shuta Takayanagi and Motohide Aoki and Tomonari Umemura and Julian J Eaton-Rye and Frances D Pitt and Mikio Tsuzuki},<br \/>\r\nurl = {http:\/\/www.sciencedirect.com\/science\/article\/pii\/S2211926418307380},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1016\/j.algal.2019.101410},<br \/>\r\nissn = {2211-9264},<br \/>\r\nyear  = {2019},<br \/>\r\ndate = {2019-01-01},<br \/>\r\njournal = {Algal Research},<br \/>\r\nvolume = {38},<br \/>\r\npages = {101410},<br \/>\r\nabstract = {Phosphate transporters, which take up not only phosphate but also arsenate, have attracted attention for phosphorus (P) resource recovery from eutrophic environments and arsenic (As) removal from polluted areas by bioremediation. The cyanobacterium Synechocystis sp. PCC 6803 can grow in a higher ratio of arsenate (150\u202fmM) to phosphate (0.2\u202fmM) in comparison with plants and microalgae. Analysis of the As resistance of the Pst1 and Pst2 phosphate transporter deletion mutants (\u0394pst1 and \u0394pst2) showed that the \u0394pst1 strain is more sensitive to arsenate under P-replete conditions but more resistant under P-deplete conditions compared with \u0394pst2 cells and wild type. This pattern in sensitivity is attributed to the levels of initial As accumulation and pst gene expression. Moreover, the ratio of phosphate uptake to arsenate uptake rates at 10\u202f\u03bcM was 7\u201310:1 in the wild type and the \u0394pst2 mutant, while that in \u0394pst1 and \u0394 pstS1 (phosphate-binding protein from the pst1 gene cluster), were about 1.5:1, suggesting a high selectivity of Pst1 for phosphate.},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('335','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_abstract\" id=\"tp_abstract_335\" style=\"display:none;\"><div class=\"tp_abstract_entry\">Phosphate transporters, which take up not only phosphate but also arsenate, have attracted attention for phosphorus (P) resource recovery from eutrophic environments and arsenic (As) removal from polluted areas by bioremediation. The cyanobacterium Synechocystis sp. PCC 6803 can grow in a higher ratio of arsenate (150\u202fmM) to phosphate (0.2\u202fmM) in comparison with plants and microalgae. Analysis of the As resistance of the Pst1 and Pst2 phosphate transporter deletion mutants (\u0394pst1 and \u0394pst2) showed that the \u0394pst1 strain is more sensitive to arsenate under P-replete conditions but more resistant under P-deplete conditions compared with \u0394pst2 cells and wild type. This pattern in sensitivity is attributed to the levels of initial As accumulation and pst gene expression. Moreover, the ratio of phosphate uptake to arsenate uptake rates at 10\u202f\u03bcM was 7\u201310:1 in the wild type and the \u0394pst2 mutant, while that in \u0394pst1 and \u0394 pstS1 (phosphate-binding protein from the pst1 gene cluster), were about 1.5:1, suggesting a high selectivity of Pst1 for phosphate.<\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('335','tp_abstract')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_335\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S2211926418307380\" title=\"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S2211926418307380\" target=\"_blank\">http:\/\/www.sciencedirect.com\/science\/article\/pii\/S2211926418307380<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1016\/j.algal.2019.101410\" title=\"Follow DOI:https:\/\/doi.org\/10.1016\/j.algal.2019.101410\" target=\"_blank\">doi:https:\/\/doi.org\/10.1016\/j.algal.2019.101410<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('335','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\"> Nagashima, Shun;  Takeda, Keisuke;  Ohno, Nobuhiko;  Ishido, Satoshi;  Aoki, Motohide;  Saitoh, Yurika;  Takada, Takumi;  Tokuyama, Takeshi;  Sugiura, Ayumu;  Fukuda, Toshifumi;  Matsushita, Nobuko;  Inatome, Ryoko;  Yanagi, Shigeru<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('336','tp_links')\" style=\"cursor:pointer;\">MITOL deletion in the brain impairs mitochondrial structure and ER tethering leading to oxidative stress<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Life Science Alliance, <\/span><span class=\"tp_pub_additional_volume\">vol. 2, <\/span><span class=\"tp_pub_additional_number\">no. 4, <\/span><span class=\"tp_pub_additional_year\">2019<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_abstract_link\"><a id=\"tp_abstract_sh_336\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('336','tp_abstract')\" title=\"Show abstract\" style=\"cursor:pointer;\">Abstract<\/a><\/span> | <span class=\"tp_resource_link\"><a id=\"tp_links_sh_336\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('336','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_336\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('336','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_336\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Nagashimae201900308,<br \/>\r\ntitle = {MITOL deletion in the brain impairs mitochondrial structure and ER tethering leading to oxidative stress},<br \/>\r\nauthor = {Shun Nagashima and Keisuke Takeda and Nobuhiko Ohno and Satoshi Ishido and Motohide Aoki and Yurika Saitoh and Takumi Takada and Takeshi Tokuyama and Ayumu Sugiura and Toshifumi Fukuda and Nobuko Matsushita and Ryoko Inatome and Shigeru Yanagi},<br \/>\r\nurl = {https:\/\/www.life-science-alliance.org\/content\/2\/4\/e201900308},<br \/>\r\ndoi = {10.26508\/lsa.201900308},<br \/>\r\nyear  = {2019},<br \/>\r\ndate = {2019-01-01},<br \/>\r\njournal = {Life Science Alliance},<br \/>\r\nvolume = {2},<br \/>\r\nnumber = {4},<br \/>\r\npublisher = {Life Science Alliance},<br \/>\r\nabstract = {Mitochondrial abnormalities are associated with developmental disorders, although a causal relationship remains largely unknown. Here, we report that increased oxidative stress in neurons by deletion of mitochondrial ubiquitin ligase MITOL causes a potential neuroinflammation including aberrant astrogliosis and microglial activation, indicating that mitochondrial abnormalities might confer a risk for inflammatory diseases in brain such as psychiatric disorders. A role of MITOL in both mitochondrial dynamics and ER-mitochondria tethering prompted us to characterize three-dimensional structures of mitochondria in vivo. In MITOL-deficient neurons, we observed a significant reduction in the ER-mitochondria contact sites, which might lead to perturbation of phospholipids transfer, consequently reduce cardiolipin biogenesis. We also found that branched large mitochondria disappeared by deletion of MITOL. These morphological abnormalities of mitochondria resulted in enhanced oxidative stress in brain, which led to astrogliosis and microglial activation partly causing abnormal behavior. In conclusion, the reduced ER-mitochondria tethering and excessive mitochondrial fission may trigger neuroinflammation through oxidative stress.},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('336','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_abstract\" id=\"tp_abstract_336\" style=\"display:none;\"><div class=\"tp_abstract_entry\">Mitochondrial abnormalities are associated with developmental disorders, although a causal relationship remains largely unknown. Here, we report that increased oxidative stress in neurons by deletion of mitochondrial ubiquitin ligase MITOL causes a potential neuroinflammation including aberrant astrogliosis and microglial activation, indicating that mitochondrial abnormalities might confer a risk for inflammatory diseases in brain such as psychiatric disorders. A role of MITOL in both mitochondrial dynamics and ER-mitochondria tethering prompted us to characterize three-dimensional structures of mitochondria in vivo. In MITOL-deficient neurons, we observed a significant reduction in the ER-mitochondria contact sites, which might lead to perturbation of phospholipids transfer, consequently reduce cardiolipin biogenesis. We also found that branched large mitochondria disappeared by deletion of MITOL. These morphological abnormalities of mitochondria resulted in enhanced oxidative stress in brain, which led to astrogliosis and microglial activation partly causing abnormal behavior. In conclusion, the reduced ER-mitochondria tethering and excessive mitochondrial fission may trigger neuroinflammation through oxidative stress.<\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('336','tp_abstract')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_336\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/www.life-science-alliance.org\/content\/2\/4\/e201900308\" title=\"https:\/\/www.life-science-alliance.org\/content\/2\/4\/e201900308\" target=\"_blank\">https:\/\/www.life-science-alliance.org\/content\/2\/4\/e201900308<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/10.26508\/lsa.201900308\" title=\"Follow DOI:10.26508\/lsa.201900308\" target=\"_blank\">doi:10.26508\/lsa.201900308<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('336','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Welcome to our laboratory&#8217;s website!!! The mission of our laboratory is to develop new analytical methods that can measure and\/or observe directly interactions between chemical substances and living cells\/organisms, and to understand molecular, cellular, individual and ecosystem-level mechanism that life respond to the chemical environmental changes. Our long-term goal is to use the knowledge gained from our research to contribute to solution of drug design or an environmental problem. Recent Pubrications<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-8","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.ls.toyaku.ac.jp\/~bioanalchem\/en\/wp-json\/wp\/v2\/pages\/8","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ls.toyaku.ac.jp\/~bioanalchem\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.ls.toyaku.ac.jp\/~bioanalchem\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.ls.toyaku.ac.jp\/~bioanalchem\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ls.toyaku.ac.jp\/~bioanalchem\/en\/wp-json\/wp\/v2\/comments?post=8"}],"version-history":[{"count":15,"href":"https:\/\/www.ls.toyaku.ac.jp\/~bioanalchem\/en\/wp-json\/wp\/v2\/pages\/8\/revisions"}],"predecessor-version":[{"id":89,"href":"https:\/\/www.ls.toyaku.ac.jp\/~bioanalchem\/en\/wp-json\/wp\/v2\/pages\/8\/revisions\/89"}],"wp:attachment":[{"href":"https:\/\/www.ls.toyaku.ac.jp\/~bioanalchem\/en\/wp-json\/wp\/v2\/media?parent=8"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}