{"id":487,"date":"2013-05-25T11:58:00","date_gmt":"2013-05-25T02:58:00","guid":{"rendered":"https:\/\/www.ls.toyaku.ac.jp\/~bioanalchem\/aoki\/?p=487"},"modified":"2018-11-10T12:08:42","modified_gmt":"2018-11-10T03:08:42","slug":"inoue_2013_publish","status":"publish","type":"post","link":"https:\/\/www.ls.toyaku.ac.jp\/~bioanalchem\/aoki\/2013\/05\/inoue_2013_publish\/","title":{"rendered":"\u5b66\u90e8\uff14\u5e74\u751f\u306e\u4e95\u4e0a\u679c\u90a3\u3055\u3093\u306e\u7814\u7a76\u6210\u679c\u8ad6\u6587\u304cJournal of Environmental Science and Health\u8a8c\u306b\u63b2\u8f09\u3055\u308c\u307e\u3057\u305f"},"content":{"rendered":"<p>\u5b66\u90e8\uff14\u5e74\u751f\uff08\u73fe \u4fee\u58eb\u8ab2\u7a0b\uff11\u5e74\u751f\uff09\u306e\u4e95\u4e0a\u679c\u90a3\u3055\u3093\u306e\u5b66\u90e8\u5352\u696d\u7814\u7a76\u306e\u6210\u679c\u8ad6\u6587\u304cJournal of Environmental Science and Health\u8a8c\u306b\u63b2\u8f09\u3055\u308c\u307e\u3057\u305f\u3002<a href=\"https:\/\/www.ls.toyaku.ac.jp\/~bioanalchem\/aoki\/\">\u3010\u751f\u4f53\u7269\u8cea\u5206\u6790\u30c1\u30fc\u30e0\u3011<\/a><\/p>\n<p class=\"tp_pub_author\">Inoue, Kana; Aoki, Motohide; Fujiwara, Kitao<\/p>\n<p class=\"tp_pub_title\"><a class=\"tp_title_link\">Protein cohesion induced by metal ions observed with fluorescence correlation spectroscopy<\/a><\/p>\n<p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_journal\">Journal of Environmental Science and Health, Part A, <\/span><span class=\"tp_pub_additional_volume\">48 <\/span><span class=\"tp_pub_additional_number\">(11), <\/span><span class=\"tp_pub_additional_pages\">pp. 1311\u20131317, <\/span><span class=\"tp_pub_additional_year\">2013<\/span>, <span class=\"tp_pub_additional_issn\">ISSN: 1093-4529<\/span>.<\/p>\n<p>Published online: 24 May 2013<\/p>\n<div class=\"sectionInfo abstractSectionHeading\">\n<div class=\"sectionHeading\">Abstract<\/div>\n<\/div>\n<div class=\"abstractSection abstractInFull\">\n<p>Nine metal ions were evaluated in the point of denaturating action of proteins. When some metal ions were added to the diluted protein solutions, aggregates appear: stronger denaturation causes the appearance of the larger-size aggregate. The size of the aggregatates are determined by fluorescence correlation spectroscopy (FCS). Green fluorescent protein (ZsGreen) and PE(phycoerythrin)-conjugated human-antibody monoclonal protein were employed as the target protein, of which solution was diluted 100\u2013500&nbsp;times and mixed with metal ions. According to this process, the denaturation power of metal ions is in the order of Mn<sup>2+<\/sup>\u2248 Fe<sup>2+<\/sup>&lt; Co<sup>2+<\/sup>&lt; Ni<sup>2+<\/sup>&lt; Tl<sup>+<\/sup>&lt; Cd<sup>2+<\/sup>&lt; Cu<sup>+<\/sup>&lt; Cu<sup>2+<\/sup>&lt; Pb<sup>2+<\/sup>for ZsGreen, and Tl<sup>+<\/sup>\u2248 Ni<sup>2+<\/sup>&lt; Cd<sup>2+<\/sup>&lt; Fe<sup>2+<\/sup>&lt; Cr<sup>3+<\/sup>\u226a Pb<sup>2+<\/sup>for PE-conjugated antibody protein. Pb<sup>2+<\/sup>exhibits the strongest power of denaturation. In the case of ZsGreen, the denaturation power of metal ions is on the order of the Irving-Williams series, which provide the coordination tendency against ligands possessing nitrogen and oxygen. The present method with FCS is effective to evaluate the denaturation power of metal ions against proteins.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u5b66\u90e8\uff14\u5e74\u751f\uff08\u73fe \u4fee\u58eb\u8ab2\u7a0b\uff11\u5e74\u751f\uff09\u306e\u4e95\u4e0a\u679c\u90a3\u3055\u3093\u306e\u5b66\u90e8\u5352\u696d\u7814\u7a76\u306e\u6210\u679c\u8ad6\u6587\u304cJournal of Environmental Science and Health\u8a8c\u306b\u63b2\u8f09\u3055\u308c\u307e\u3057\u305f\u3002\u3010\u751f\u4f53\u7269\u8cea\u5206\u6790\u30c1\u30fc\u30e0\u3011 Inoue, Ka &hellip; <a href=\"https:\/\/www.ls.toyaku.ac.jp\/~bioanalchem\/aoki\/2013\/05\/inoue_2013_publish\/\" class=\"more-link\"><span class=\"screen-reader-text\">&#8220;\u5b66\u90e8\uff14\u5e74\u751f\u306e\u4e95\u4e0a\u679c\u90a3\u3055\u3093\u306e\u7814\u7a76\u6210\u679c\u8ad6\u6587\u304cJournal of Environmental Science and Health\u8a8c\u306b\u63b2\u8f09\u3055\u308c\u307e\u3057\u305f&#8221; \u306e<\/span>\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3,4],"tags":[],"class_list":["post-487","post","type-post","status-publish","format-standard","hentry","category-3","category-4"],"jetpack_featured_media_url":"","_links":{"self":[{"href":"https:\/\/www.ls.toyaku.ac.jp\/~bioanalchem\/aoki\/wp-json\/wp\/v2\/posts\/487","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ls.toyaku.ac.jp\/~bioanalchem\/aoki\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.ls.toyaku.ac.jp\/~bioanalchem\/aoki\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.ls.toyaku.ac.jp\/~bioanalchem\/aoki\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ls.toyaku.ac.jp\/~bioanalchem\/aoki\/wp-json\/wp\/v2\/comments?post=487"}],"version-history":[{"count":3,"href":"https:\/\/www.ls.toyaku.ac.jp\/~bioanalchem\/aoki\/wp-json\/wp\/v2\/posts\/487\/revisions"}],"predecessor-version":[{"id":490,"href":"https:\/\/www.ls.toyaku.ac.jp\/~bioanalchem\/aoki\/wp-json\/wp\/v2\/posts\/487\/revisions\/490"}],"wp:attachment":[{"href":"https:\/\/www.ls.toyaku.ac.jp\/~bioanalchem\/aoki\/wp-json\/wp\/v2\/media?parent=487"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.ls.toyaku.ac.jp\/~bioanalchem\/aoki\/wp-json\/wp\/v2\/categories?post=487"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.ls.toyaku.ac.jp\/~bioanalchem\/aoki\/wp-json\/wp\/v2\/tags?post=487"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}